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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 8-124543176-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=8&pos=124543176&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "8",
      "pos": 124543176,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_005005.3",
      "consequences": [
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFB9",
          "gene_hgnc_id": 7704,
          "hgvs_c": "c.191T>C",
          "hgvs_p": "p.Leu64Pro",
          "transcript": "NM_005005.3",
          "protein_id": "NP_004996.1",
          "transcript_support_level": null,
          "aa_start": 64,
          "aa_end": null,
          "aa_length": 179,
          "cds_start": 191,
          "cds_end": null,
          "cds_length": 540,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000276689.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_005005.3"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFB9",
          "gene_hgnc_id": 7704,
          "hgvs_c": "c.191T>C",
          "hgvs_p": "p.Leu64Pro",
          "transcript": "ENST00000276689.8",
          "protein_id": "ENSP00000276689.3",
          "transcript_support_level": 1,
          "aa_start": 64,
          "aa_end": null,
          "aa_length": 179,
          "cds_start": 191,
          "cds_end": null,
          "cds_length": 540,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_005005.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000276689.8"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFB9",
          "gene_hgnc_id": 7704,
          "hgvs_c": "c.290T>C",
          "hgvs_p": "p.Leu97Pro",
          "transcript": "ENST00000901305.1",
          "protein_id": "ENSP00000571364.1",
          "transcript_support_level": null,
          "aa_start": 97,
          "aa_end": null,
          "aa_length": 212,
          "cds_start": 290,
          "cds_end": null,
          "cds_length": 639,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901305.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFB9",
          "gene_hgnc_id": 7704,
          "hgvs_c": "c.191T>C",
          "hgvs_p": "p.Leu64Pro",
          "transcript": "ENST00000518008.5",
          "protein_id": "ENSP00000428282.1",
          "transcript_support_level": 2,
          "aa_start": 64,
          "aa_end": null,
          "aa_length": 178,
          "cds_start": 191,
          "cds_end": null,
          "cds_length": 537,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000518008.5"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFB9",
          "gene_hgnc_id": 7704,
          "hgvs_c": "c.191T>C",
          "hgvs_p": "p.Leu64Pro",
          "transcript": "ENST00000928469.1",
          "protein_id": "ENSP00000598528.1",
          "transcript_support_level": null,
          "aa_start": 64,
          "aa_end": null,
          "aa_length": 178,
          "cds_start": 191,
          "cds_end": null,
          "cds_length": 537,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928469.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFB9",
          "gene_hgnc_id": 7704,
          "hgvs_c": "c.191T>C",
          "hgvs_p": "p.Leu64Pro",
          "transcript": "ENST00000928470.1",
          "protein_id": "ENSP00000598529.1",
          "transcript_support_level": null,
          "aa_start": 64,
          "aa_end": null,
          "aa_length": 177,
          "cds_start": 191,
          "cds_end": null,
          "cds_length": 534,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928470.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFB9",
          "gene_hgnc_id": 7704,
          "hgvs_c": "c.191T>C",
          "hgvs_p": "p.Leu64Pro",
          "transcript": "ENST00000901304.1",
          "protein_id": "ENSP00000571363.1",
          "transcript_support_level": null,
          "aa_start": 64,
          "aa_end": null,
          "aa_length": 176,
          "cds_start": 191,
          "cds_end": null,
          "cds_length": 531,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901304.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFB9",
          "gene_hgnc_id": 7704,
          "hgvs_c": "c.158T>C",
          "hgvs_p": "p.Leu53Pro",
          "transcript": "NM_001311168.2",
          "protein_id": "NP_001298097.1",
          "transcript_support_level": null,
          "aa_start": 53,
          "aa_end": null,
          "aa_length": 168,
          "cds_start": 158,
          "cds_end": null,
          "cds_length": 507,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001311168.2"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFB9",
          "gene_hgnc_id": 7704,
          "hgvs_c": "c.158T>C",
          "hgvs_p": "p.Leu53Pro",
          "transcript": "ENST00000517367.1",
          "protein_id": "ENSP00000430322.1",
          "transcript_support_level": 3,
          "aa_start": 53,
          "aa_end": null,
          "aa_length": 168,
          "cds_start": 158,
          "cds_end": null,
          "cds_length": 507,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000517367.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFB9",
          "gene_hgnc_id": 7704,
          "hgvs_c": "c.158T>C",
          "hgvs_p": "p.Leu53Pro",
          "transcript": "ENST00000928471.1",
          "protein_id": "ENSP00000598530.1",
          "transcript_support_level": null,
          "aa_start": 53,
          "aa_end": null,
          "aa_length": 167,
          "cds_start": 158,
          "cds_end": null,
          "cds_length": 504,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928471.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFB9",
          "gene_hgnc_id": 7704,
          "hgvs_c": "c.191T>C",
          "hgvs_p": "p.Leu64Pro",
          "transcript": "ENST00000928472.1",
          "protein_id": "ENSP00000598531.1",
          "transcript_support_level": null,
          "aa_start": 64,
          "aa_end": null,
          "aa_length": 141,
          "cds_start": 191,
          "cds_end": null,
          "cds_length": 426,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928472.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFB9",
          "gene_hgnc_id": 7704,
          "hgvs_c": "c.191T>C",
          "hgvs_p": "p.Leu64Pro",
          "transcript": "ENST00000606244.2",
          "protein_id": "ENSP00000497750.1",
          "transcript_support_level": 6,
          "aa_start": 64,
          "aa_end": null,
          "aa_length": 138,
          "cds_start": 191,
          "cds_end": null,
          "cds_length": 417,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000606244.2"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFB9",
          "gene_hgnc_id": 7704,
          "hgvs_c": "c.62T>C",
          "hgvs_p": "p.Leu21Pro",
          "transcript": "NM_001278646.2",
          "protein_id": "NP_001265575.1",
          "transcript_support_level": null,
          "aa_start": 21,
          "aa_end": null,
          "aa_length": 136,
          "cds_start": 62,
          "cds_end": null,
          "cds_length": 411,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001278646.2"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFB9",
          "gene_hgnc_id": 7704,
          "hgvs_c": "c.23T>C",
          "hgvs_p": "p.Leu8Pro",
          "transcript": "NM_001278645.2",
          "protein_id": "NP_001265574.1",
          "transcript_support_level": null,
          "aa_start": 8,
          "aa_end": null,
          "aa_length": 123,
          "cds_start": 23,
          "cds_end": null,
          "cds_length": 372,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001278645.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFB9",
          "gene_hgnc_id": 7704,
          "hgvs_c": "n.1271T>C",
          "hgvs_p": null,
          "transcript": "ENST00000518657.6",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000518657.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFB9",
          "gene_hgnc_id": 7704,
          "hgvs_c": "n.191T>C",
          "hgvs_p": null,
          "transcript": "ENST00000522532.6",
          "protein_id": "ENSP00000431115.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000522532.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFB9",
          "gene_hgnc_id": 7704,
          "hgvs_c": "n.216T>C",
          "hgvs_p": null,
          "transcript": "ENST00000524241.2",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000524241.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFB9",
          "gene_hgnc_id": 7704,
          "hgvs_c": "n.*45T>C",
          "hgvs_p": null,
          "transcript": "ENST00000676713.1",
          "protein_id": "ENSP00000504795.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
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          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000676713.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFB9",
          "gene_hgnc_id": 7704,
          "hgvs_c": "n.191T>C",
          "hgvs_p": null,
          "transcript": "ENST00000677021.1",
          "protein_id": "ENSP00000504235.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000677021.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFB9",
          "gene_hgnc_id": 7704,
          "hgvs_c": "n.210T>C",
          "hgvs_p": null,
          "transcript": "ENST00000677782.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000677782.1"
        },
        {
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          "biotype": "nonsense_mediated_decay",
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        {
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          "protein_coding": false,
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          "consequences": [
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          "gene_symbol": "NDUFB9",
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          "hgvs_c": "n.*45T>C",
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          "transcript": "ENST00000677950.1",
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          "biotype": "nonsense_mediated_decay",
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        {
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          "protein_coding": false,
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          "consequences": [
            "3_prime_UTR_variant"
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          "exon_count": 5,
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          "gene_symbol": "NDUFB9",
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          "biotype": "nonsense_mediated_decay",
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        {
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          "protein_coding": false,
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          "consequences": [
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        {
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          "gene_symbol": "NDUFB9",
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          "hgvs_c": "n.-68T>C",
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          "transcript": "ENST00000678801.1",
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          "biotype": "retained_intron",
          "feature": "ENST00000678801.1"
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      ],
      "gene_symbol": "NDUFB9",
      "gene_hgnc_id": 7704,
      "dbsnp": "rs776388520",
      "frequency_reference_population": 0.0000055758765,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 9,
      "gnomad_exomes_af": 0.00000478843,
      "gnomad_genomes_af": 0.0000131373,
      "gnomad_exomes_ac": 7,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9589285850524902,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.885,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9809,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.39,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.584,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 7,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PP3_Strong,PP5",
      "acmg_by_gene": [
        {
          "score": 7,
          "benign_score": 0,
          "pathogenic_score": 7,
          "criteria": [
            "PM2",
            "PP3_Strong",
            "PP5"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_005005.3",
          "gene_symbol": "NDUFB9",
          "hgnc_id": 7704,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,Unknown",
          "hgvs_c": "c.191T>C",
          "hgvs_p": "p.Leu64Pro"
        }
      ],
      "clinvar_disease": " nuclear type 24,Mitochondrial complex I deficiency",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "Mitochondrial complex I deficiency, nuclear type 24",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}
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