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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 8-144083951-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=8&pos=144083951&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "8",
      "pos": 144083951,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "ENST00000355091.9",
      "consequences": [
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GPAA1",
          "gene_hgnc_id": 4446,
          "hgvs_c": "c.527G>C",
          "hgvs_p": "p.Trp176Ser",
          "transcript": "NM_003801.4",
          "protein_id": "NP_003792.1",
          "transcript_support_level": null,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 621,
          "cds_start": 527,
          "cds_end": null,
          "cds_length": 1866,
          "cdna_start": 624,
          "cdna_end": null,
          "cdna_length": 2054,
          "mane_select": "ENST00000355091.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GPAA1",
          "gene_hgnc_id": 4446,
          "hgvs_c": "c.527G>C",
          "hgvs_p": "p.Trp176Ser",
          "transcript": "ENST00000355091.9",
          "protein_id": "ENSP00000347206.4",
          "transcript_support_level": 1,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 621,
          "cds_start": 527,
          "cds_end": null,
          "cds_length": 1866,
          "cdna_start": 624,
          "cdna_end": null,
          "cdna_length": 2054,
          "mane_select": "NM_003801.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GPAA1",
          "gene_hgnc_id": 4446,
          "hgvs_c": "c.347G>C",
          "hgvs_p": "p.Trp116Ser",
          "transcript": "ENST00000361036.11",
          "protein_id": "ENSP00000354316.6",
          "transcript_support_level": 1,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 561,
          "cds_start": 347,
          "cds_end": null,
          "cds_length": 1686,
          "cdna_start": 489,
          "cdna_end": null,
          "cdna_length": 1919,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000290230",
          "gene_hgnc_id": null,
          "hgvs_c": "n.*249G>C",
          "hgvs_p": null,
          "transcript": "ENST00000703646.1",
          "protein_id": "ENSP00000515414.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1531,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000290230",
          "gene_hgnc_id": null,
          "hgvs_c": "n.*249G>C",
          "hgvs_p": null,
          "transcript": "ENST00000703646.1",
          "protein_id": "ENSP00000515414.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1531,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GPAA1",
          "gene_hgnc_id": 4446,
          "hgvs_c": "c.527G>C",
          "hgvs_p": "p.Trp176Ser",
          "transcript": "ENST00000703648.1",
          "protein_id": "ENSP00000515415.1",
          "transcript_support_level": null,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 626,
          "cds_start": 527,
          "cds_end": null,
          "cds_length": 1881,
          "cdna_start": 662,
          "cdna_end": null,
          "cdna_length": 2103,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GPAA1",
          "gene_hgnc_id": 4446,
          "hgvs_c": "c.527G>C",
          "hgvs_p": "p.Trp176Ser",
          "transcript": "ENST00000703649.1",
          "protein_id": "ENSP00000515416.1",
          "transcript_support_level": null,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 624,
          "cds_start": 527,
          "cds_end": null,
          "cds_length": 1875,
          "cdna_start": 601,
          "cdna_end": null,
          "cdna_length": 2033,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GPAA1",
          "gene_hgnc_id": 4446,
          "hgvs_c": "c.527G>C",
          "hgvs_p": "p.Trp176Ser",
          "transcript": "ENST00000703620.1",
          "protein_id": "ENSP00000515404.1",
          "transcript_support_level": null,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 619,
          "cds_start": 527,
          "cds_end": null,
          "cds_length": 1860,
          "cdna_start": 642,
          "cdna_end": null,
          "cdna_length": 2066,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GPAA1",
          "gene_hgnc_id": 4446,
          "hgvs_c": "c.527G>C",
          "hgvs_p": "p.Trp176Ser",
          "transcript": "ENST00000703633.1",
          "protein_id": "ENSP00000515410.1",
          "transcript_support_level": null,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 607,
          "cds_start": 527,
          "cds_end": null,
          "cds_length": 1824,
          "cdna_start": 603,
          "cdna_end": null,
          "cdna_length": 1991,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GPAA1",
          "gene_hgnc_id": 4446,
          "hgvs_c": "c.527G>C",
          "hgvs_p": "p.Trp176Ser",
          "transcript": "ENST00000703621.1",
          "protein_id": "ENSP00000515405.1",
          "transcript_support_level": null,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 606,
          "cds_start": 527,
          "cds_end": null,
          "cds_length": 1821,
          "cdna_start": 648,
          "cdna_end": null,
          "cdna_length": 2033,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GPAA1",
          "gene_hgnc_id": 4446,
          "hgvs_c": "c.527G>C",
          "hgvs_p": "p.Trp176Ser",
          "transcript": "ENST00000704807.1",
          "protein_id": "ENSP00000516044.1",
          "transcript_support_level": null,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 604,
          "cds_start": 527,
          "cds_end": null,
          "cds_length": 1815,
          "cdna_start": 624,
          "cdna_end": null,
          "cdna_length": 2001,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GPAA1",
          "gene_hgnc_id": 4446,
          "hgvs_c": "c.527G>C",
          "hgvs_p": "p.Trp176Ser",
          "transcript": "ENST00000703622.1",
          "protein_id": "ENSP00000515406.1",
          "transcript_support_level": null,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 580,
          "cds_start": 527,
          "cds_end": null,
          "cds_length": 1743,
          "cdna_start": 660,
          "cdna_end": null,
          "cdna_length": 1899,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GPAA1",
          "gene_hgnc_id": 4446,
          "hgvs_c": "c.347G>C",
          "hgvs_p": "p.Trp116Ser",
          "transcript": "ENST00000704806.1",
          "protein_id": "ENSP00000516043.1",
          "transcript_support_level": null,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 546,
          "cds_start": 347,
          "cds_end": null,
          "cds_length": 1641,
          "cdna_start": 456,
          "cdna_end": null,
          "cdna_length": 1841,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GPAA1",
          "gene_hgnc_id": 4446,
          "hgvs_c": "c.161G>C",
          "hgvs_p": "p.Trp54Ser",
          "transcript": "ENST00000704794.1",
          "protein_id": "ENSP00000516039.1",
          "transcript_support_level": null,
          "aa_start": 54,
          "aa_end": null,
          "aa_length": 499,
          "cds_start": 161,
          "cds_end": null,
          "cds_length": 1500,
          "cdna_start": 328,
          "cdna_end": null,
          "cdna_length": 1758,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GPAA1",
          "gene_hgnc_id": 4446,
          "hgvs_c": "c.161G>C",
          "hgvs_p": "p.Trp54Ser",
          "transcript": "ENST00000703635.1",
          "protein_id": "ENSP00000515412.1",
          "transcript_support_level": null,
          "aa_start": 54,
          "aa_end": null,
          "aa_length": 431,
          "cds_start": 161,
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          "cds_length": 1296,
          "cdna_start": 161,
          "cdna_end": null,
          "cdna_length": 1452,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GPAA1",
          "gene_hgnc_id": 4446,
          "hgvs_c": "c.311G>C",
          "hgvs_p": "p.Trp104Ser",
          "transcript": "ENST00000525087.6",
          "protein_id": "ENSP00000434485.3",
          "transcript_support_level": 5,
          "aa_start": 104,
          "aa_end": null,
          "aa_length": 347,
          "cds_start": 311,
          "cds_end": null,
          "cds_length": 1044,
          "cdna_start": 428,
          "cdna_end": null,
          "cdna_length": 1161,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GPAA1",
          "gene_hgnc_id": 4446,
          "hgvs_c": "n.922G>C",
          "hgvs_p": null,
          "transcript": "ENST00000525308.2",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2766,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GPAA1",
          "gene_hgnc_id": 4446,
          "hgvs_c": "n.*69G>C",
          "hgvs_p": null,
          "transcript": "ENST00000526341.6",
          "protein_id": "ENSP00000515386.1",
          "transcript_support_level": 5,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2110,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GPAA1",
          "gene_hgnc_id": 4446,
          "hgvs_c": "n.527G>C",
          "hgvs_p": null,
          "transcript": "ENST00000527144.6",
          "protein_id": "ENSP00000515403.1",
          "transcript_support_level": 3,
          "aa_start": null,
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          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 1920,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GPAA1",
          "gene_hgnc_id": 4446,
          "hgvs_c": "n.527G>C",
          "hgvs_p": null,
          "transcript": "ENST00000528073.6",
          "protein_id": "ENSP00000435119.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2010,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GPAA1",
          "gene_hgnc_id": 4446,
          "hgvs_c": "n.451G>C",
          "hgvs_p": null,
          "transcript": "ENST00000529503.6",
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      ],
      "gene_symbol": "GPAA1",
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      "dbsnp": "rs782220208",
      "frequency_reference_population": 0.0000075253706,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 11,
      "gnomad_exomes_af": 0.00000752537,
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      "gnomad_exomes_ac": 11,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9767976999282837,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.03999999910593033,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.751,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9731,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.49,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 9.07,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.04,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 8,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PP3_Strong,PP5_Moderate",
      "acmg_by_gene": [
        {
          "score": 8,
          "benign_score": 0,
          "pathogenic_score": 8,
          "criteria": [
            "PM2",
            "PP3_Strong",
            "PP5_Moderate"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "ENST00000355091.9",
          "gene_symbol": "GPAA1",
          "hgnc_id": 4446,
          "effects": [
            "missense_variant"
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          "inheritance_mode": "AR",
          "hgvs_c": "c.527G>C",
          "hgvs_p": "p.Trp176Ser"
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        {
          "score": 8,
          "benign_score": 0,
          "pathogenic_score": 8,
          "criteria": [
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            "PP3_Strong",
            "PP5_Moderate"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "ENST00000703646.1",
          "gene_symbol": "ENSG00000290230",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.*249G>C",
          "hgvs_p": null
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      ],
      "clinvar_disease": "Glycosylphosphatidylinositol biosynthesis defect 15",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LP:1",
      "phenotype_combined": "Glycosylphosphatidylinositol biosynthesis defect 15",
      "pathogenicity_classification_combined": "Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}