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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 8-144512480-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=8&pos=144512480&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "8",
      "pos": 144512480,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "ENST00000617875.6",
      "consequences": [
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2967G>A",
          "hgvs_p": "p.Met989Ile",
          "transcript": "NM_004260.4",
          "protein_id": "NP_004251.4",
          "transcript_support_level": null,
          "aa_start": 989,
          "aa_end": null,
          "aa_length": 1208,
          "cds_start": 2967,
          "cds_end": null,
          "cds_length": 3627,
          "cdna_start": 3016,
          "cdna_end": null,
          "cdna_length": 3819,
          "mane_select": "ENST00000617875.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2967G>A",
          "hgvs_p": "p.Met989Ile",
          "transcript": "ENST00000617875.6",
          "protein_id": "ENSP00000482313.2",
          "transcript_support_level": 1,
          "aa_start": 989,
          "aa_end": null,
          "aa_length": 1208,
          "cds_start": 2967,
          "cds_end": null,
          "cds_length": 3627,
          "cdna_start": 3016,
          "cdna_end": null,
          "cdna_length": 3819,
          "mane_select": "NM_004260.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.1896G>A",
          "hgvs_p": "p.Met632Ile",
          "transcript": "ENST00000621189.4",
          "protein_id": "ENSP00000483145.1",
          "transcript_support_level": 1,
          "aa_start": 632,
          "aa_end": null,
          "aa_length": 851,
          "cds_start": 1896,
          "cds_end": null,
          "cds_length": 2556,
          "cdna_start": 3093,
          "cdna_end": null,
          "cdna_length": 3896,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3042G>A",
          "hgvs_p": "p.Met1014Ile",
          "transcript": "NM_001413019.1",
          "protein_id": "NP_001399948.1",
          "transcript_support_level": null,
          "aa_start": 1014,
          "aa_end": null,
          "aa_length": 1233,
          "cds_start": 3042,
          "cds_end": null,
          "cds_length": 3702,
          "cdna_start": 3091,
          "cdna_end": null,
          "cdna_length": 3894,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2967G>A",
          "hgvs_p": "p.Met989Ile",
          "transcript": "NM_001413036.1",
          "protein_id": "NP_001399965.1",
          "transcript_support_level": null,
          "aa_start": 989,
          "aa_end": null,
          "aa_length": 1211,
          "cds_start": 2967,
          "cds_end": null,
          "cds_length": 3636,
          "cdna_start": 3016,
          "cdna_end": null,
          "cdna_length": 3828,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2937G>A",
          "hgvs_p": "p.Met979Ile",
          "transcript": "NM_001413039.1",
          "protein_id": "NP_001399968.1",
          "transcript_support_level": null,
          "aa_start": 979,
          "aa_end": null,
          "aa_length": 1198,
          "cds_start": 2937,
          "cds_end": null,
          "cds_length": 3597,
          "cdna_start": 2986,
          "cdna_end": null,
          "cdna_length": 3789,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2871G>A",
          "hgvs_p": "p.Met957Ile",
          "transcript": "NM_001413020.1",
          "protein_id": "NP_001399949.1",
          "transcript_support_level": null,
          "aa_start": 957,
          "aa_end": null,
          "aa_length": 1176,
          "cds_start": 2871,
          "cds_end": null,
          "cds_length": 3531,
          "cdna_start": 2920,
          "cdna_end": null,
          "cdna_length": 3723,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2838G>A",
          "hgvs_p": "p.Met946Ile",
          "transcript": "NM_001413025.1",
          "protein_id": "NP_001399954.1",
          "transcript_support_level": null,
          "aa_start": 946,
          "aa_end": null,
          "aa_length": 1165,
          "cds_start": 2838,
          "cds_end": null,
          "cds_length": 3498,
          "cdna_start": 2887,
          "cdna_end": null,
          "cdna_length": 3690,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2835G>A",
          "hgvs_p": "p.Met945Ile",
          "transcript": "NM_001413033.1",
          "protein_id": "NP_001399962.1",
          "transcript_support_level": null,
          "aa_start": 945,
          "aa_end": null,
          "aa_length": 1164,
          "cds_start": 2835,
          "cds_end": null,
          "cds_length": 3495,
          "cdna_start": 2884,
          "cdna_end": null,
          "cdna_length": 3687,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2769G>A",
          "hgvs_p": "p.Met923Ile",
          "transcript": "NM_001413018.1",
          "protein_id": "NP_001399947.1",
          "transcript_support_level": null,
          "aa_start": 923,
          "aa_end": null,
          "aa_length": 1142,
          "cds_start": 2769,
          "cds_end": null,
          "cds_length": 3429,
          "cdna_start": 2818,
          "cdna_end": null,
          "cdna_length": 3621,
          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
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          "exon_count": 22,
          "intron_rank": null,
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          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2673G>A",
          "hgvs_p": "p.Met891Ile",
          "transcript": "NM_001413017.1",
          "protein_id": "NP_001399946.1",
          "transcript_support_level": null,
          "aa_start": 891,
          "aa_end": null,
          "aa_length": 1110,
          "cds_start": 2673,
          "cds_end": null,
          "cds_length": 3333,
          "cdna_start": 2722,
          "cdna_end": null,
          "cdna_length": 3525,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2616G>A",
          "hgvs_p": "p.Met872Ile",
          "transcript": "NM_001413029.1",
          "protein_id": "NP_001399958.1",
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          "cds_start": 2616,
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          "cdna_start": 2665,
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          "mane_select": null,
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        },
        {
          "aa_ref": "M",
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          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 15,
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          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.1971G>A",
          "hgvs_p": "p.Met657Ile",
          "transcript": "NM_001413023.1",
          "protein_id": "NP_001399952.1",
          "transcript_support_level": null,
          "aa_start": 657,
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          "cds_start": 1971,
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          "cdna_start": 3156,
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        },
        {
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 15,
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          "exon_count": 19,
          "intron_rank": null,
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          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.1905G>A",
          "hgvs_p": "p.Met635Ile",
          "transcript": "NM_001413041.1",
          "protein_id": "NP_001399970.1",
          "transcript_support_level": null,
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          "aa_end": null,
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          "cds_start": 1905,
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        },
        {
          "aa_ref": "M",
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          ],
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          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.1896G>A",
          "hgvs_p": "p.Met632Ile",
          "transcript": "NM_001413021.1",
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          "feature": null
        },
        {
          "aa_ref": "M",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_count": 20,
          "intron_rank": null,
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          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.1896G>A",
          "hgvs_p": "p.Met632Ile",
          "transcript": "NM_001413022.1",
          "protein_id": "NP_001399951.1",
          "transcript_support_level": null,
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        },
        {
          "aa_ref": "M",
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          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
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          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.1896G>A",
          "hgvs_p": "p.Met632Ile",
          "transcript": "NM_001413024.1",
          "protein_id": "NP_001399953.1",
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        },
        {
          "aa_ref": "M",
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          ],
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          "gene_symbol": "RECQL4",
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          "hgvs_c": "c.1896G>A",
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        },
        {
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          ],
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          "intron_rank": null,
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          "gene_symbol": "RECQL4",
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          "hgvs_c": "c.1896G>A",
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          "transcript": "NM_001413034.1",
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        },
        {
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          "consequences": [
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          ],
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          "exon_count": 21,
          "intron_rank": null,
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          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.1896G>A",
          "hgvs_p": "p.Met632Ile",
          "transcript": "NM_001413035.1",
          "protein_id": "NP_001399964.1",
          "transcript_support_level": null,
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          "cdna_start": 2913,
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          "mane_select": null,
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          "feature": null
        },
        {
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
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          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "RECQL4",
      "gene_hgnc_id": 9949,
      "dbsnp": "rs200018416",
      "frequency_reference_population": 0.0004217206,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 680,
      "gnomad_exomes_af": 0.000435596,
      "gnomad_genomes_af": 0.000288763,
      "gnomad_exomes_ac": 636,
      "gnomad_genomes_ac": 44,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.07414659857749939,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": 0.276,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.33,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.222,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000617875.6",
          "gene_symbol": "RECQL4",
          "hgnc_id": 9949,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2967G>A",
          "hgvs_p": "p.Met989Ile"
        },
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000580385.1",
          "gene_symbol": "ENSG00000265393",
          "hgnc_id": null,
          "effects": [
            "upstream_gene_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.-87C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Baller-Gerold syndrome,Hereditary cancer-predisposing syndrome,Rapadilino syndrome,Rothmund-Thomson syndrome type 2,not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:6",
      "phenotype_combined": "Baller-Gerold syndrome|not provided|Hereditary cancer-predisposing syndrome|Baller-Gerold syndrome;Rapadilino syndrome;Rothmund-Thomson syndrome type 2",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}