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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 8-27518053-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=8&pos=27518053&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "8",
      "pos": 27518053,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000521400.6",
      "consequences": [
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "c.926G>A",
          "hgvs_p": "p.Cys309Tyr",
          "transcript": "NM_001979.6",
          "protein_id": "NP_001970.2",
          "transcript_support_level": null,
          "aa_start": 309,
          "aa_end": null,
          "aa_length": 555,
          "cds_start": 926,
          "cds_end": null,
          "cds_length": 1668,
          "cdna_start": 992,
          "cdna_end": null,
          "cdna_length": 2776,
          "mane_select": "ENST00000521400.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "c.926G>A",
          "hgvs_p": "p.Cys309Tyr",
          "transcript": "ENST00000521400.6",
          "protein_id": "ENSP00000430269.1",
          "transcript_support_level": 1,
          "aa_start": 309,
          "aa_end": null,
          "aa_length": 555,
          "cds_start": 926,
          "cds_end": null,
          "cds_length": 1668,
          "cdna_start": 992,
          "cdna_end": null,
          "cdna_length": 2776,
          "mane_select": "NM_001979.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "n.1010G>A",
          "hgvs_p": null,
          "transcript": "ENST00000520623.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1923,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "c.926G>A",
          "hgvs_p": "p.Cys309Tyr",
          "transcript": "NM_001414016.1",
          "protein_id": "NP_001400945.1",
          "transcript_support_level": null,
          "aa_start": 309,
          "aa_end": null,
          "aa_length": 553,
          "cds_start": 926,
          "cds_end": null,
          "cds_length": 1662,
          "cdna_start": 992,
          "cdna_end": null,
          "cdna_length": 2686,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "c.926G>A",
          "hgvs_p": "p.Cys309Tyr",
          "transcript": "NM_001414017.1",
          "protein_id": "NP_001400946.1",
          "transcript_support_level": null,
          "aa_start": 309,
          "aa_end": null,
          "aa_length": 550,
          "cds_start": 926,
          "cds_end": null,
          "cds_length": 1653,
          "cdna_start": 992,
          "cdna_end": null,
          "cdna_length": 2439,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "c.926G>A",
          "hgvs_p": "p.Cys309Tyr",
          "transcript": "NM_001414018.1",
          "protein_id": "NP_001400947.1",
          "transcript_support_level": null,
          "aa_start": 309,
          "aa_end": null,
          "aa_length": 530,
          "cds_start": 926,
          "cds_end": null,
          "cds_length": 1593,
          "cdna_start": 992,
          "cdna_end": null,
          "cdna_length": 2330,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "c.830G>A",
          "hgvs_p": "p.Cys277Tyr",
          "transcript": "NM_001414019.1",
          "protein_id": "NP_001400948.1",
          "transcript_support_level": null,
          "aa_start": 277,
          "aa_end": null,
          "aa_length": 523,
          "cds_start": 830,
          "cds_end": null,
          "cds_length": 1572,
          "cdna_start": 896,
          "cdna_end": null,
          "cdna_length": 2035,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "c.830G>A",
          "hgvs_p": "p.Cys277Tyr",
          "transcript": "ENST00000518379.5",
          "protein_id": "ENSP00000427956.1",
          "transcript_support_level": 5,
          "aa_start": 277,
          "aa_end": null,
          "aa_length": 523,
          "cds_start": 830,
          "cds_end": null,
          "cds_length": 1572,
          "cdna_start": 847,
          "cdna_end": null,
          "cdna_length": 1959,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "c.803G>A",
          "hgvs_p": "p.Cys268Tyr",
          "transcript": "NM_001414020.1",
          "protein_id": "NP_001400949.1",
          "transcript_support_level": null,
          "aa_start": 268,
          "aa_end": null,
          "aa_length": 514,
          "cds_start": 803,
          "cds_end": null,
          "cds_length": 1545,
          "cdna_start": 869,
          "cdna_end": null,
          "cdna_length": 2008,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "c.767G>A",
          "hgvs_p": "p.Cys256Tyr",
          "transcript": "NM_001256482.2",
          "protein_id": "NP_001243411.1",
          "transcript_support_level": null,
          "aa_start": 256,
          "aa_end": null,
          "aa_length": 502,
          "cds_start": 767,
          "cds_end": null,
          "cds_length": 1509,
          "cdna_start": 954,
          "cdna_end": null,
          "cdna_length": 2738,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "c.767G>A",
          "hgvs_p": "p.Cys256Tyr",
          "transcript": "NM_001256484.2",
          "protein_id": "NP_001243413.1",
          "transcript_support_level": null,
          "aa_start": 256,
          "aa_end": null,
          "aa_length": 502,
          "cds_start": 767,
          "cds_end": null,
          "cds_length": 1509,
          "cdna_start": 990,
          "cdna_end": null,
          "cdna_length": 2774,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "c.767G>A",
          "hgvs_p": "p.Cys256Tyr",
          "transcript": "ENST00000380476.7",
          "protein_id": "ENSP00000369843.3",
          "transcript_support_level": 2,
          "aa_start": 256,
          "aa_end": null,
          "aa_length": 502,
          "cds_start": 767,
          "cds_end": null,
          "cds_length": 1509,
          "cdna_start": 931,
          "cdna_end": null,
          "cdna_length": 2064,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "c.728G>A",
          "hgvs_p": "p.Cys243Tyr",
          "transcript": "NM_001256483.2",
          "protein_id": "NP_001243412.1",
          "transcript_support_level": null,
          "aa_start": 243,
          "aa_end": null,
          "aa_length": 489,
          "cds_start": 728,
          "cds_end": null,
          "cds_length": 1470,
          "cdna_start": 907,
          "cdna_end": null,
          "cdna_length": 2691,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "c.728G>A",
          "hgvs_p": "p.Cys243Tyr",
          "transcript": "ENST00000521780.5",
          "protein_id": "ENSP00000430302.1",
          "transcript_support_level": 2,
          "aa_start": 243,
          "aa_end": null,
          "aa_length": 489,
          "cds_start": 728,
          "cds_end": null,
          "cds_length": 1470,
          "cdna_start": 899,
          "cdna_end": null,
          "cdna_length": 2038,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "c.926G>A",
          "hgvs_p": "p.Cys309Tyr",
          "transcript": "NM_001414021.1",
          "protein_id": "NP_001400950.1",
          "transcript_support_level": null,
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          "aa_end": null,
          "aa_length": 466,
          "cds_start": 926,
          "cds_end": null,
          "cds_length": 1401,
          "cdna_start": 992,
          "cdna_end": null,
          "cdna_length": 1934,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "c.377G>A",
          "hgvs_p": "p.Cys126Tyr",
          "transcript": "ENST00000517536.5",
          "protein_id": "ENSP00000428875.1",
          "transcript_support_level": 5,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 372,
          "cds_start": 377,
          "cds_end": null,
          "cds_length": 1119,
          "cdna_start": 461,
          "cdna_end": null,
          "cdna_length": 1600,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "c.800G>A",
          "hgvs_p": "p.Cys267Tyr",
          "transcript": "ENST00000521684.1",
          "protein_id": "ENSP00000428191.1",
          "transcript_support_level": 3,
          "aa_start": 267,
          "aa_end": null,
          "aa_length": 310,
          "cds_start": 800,
          "cds_end": null,
          "cds_length": 933,
          "cdna_start": 802,
          "cdna_end": null,
          "cdna_length": 935,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "n.446G>A",
          "hgvs_p": null,
          "transcript": "ENST00000523326.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 557,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "n.926G>A",
          "hgvs_p": null,
          "transcript": "ENST00000850871.1",
          "protein_id": "ENSP00000520956.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2063,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "n.992G>A",
          "hgvs_p": null,
          "transcript": "NR_182231.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2097,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "n.992G>A",
          "hgvs_p": null,
          "transcript": "NR_182232.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2120,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX2",
          "gene_hgnc_id": 3402,
          "hgvs_c": "n.992G>A",
          "hgvs_p": null,
          "transcript": "NR_182233.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2126,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "EPHX2",
      "gene_hgnc_id": 3402,
      "dbsnp": "rs1220811349",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": 0,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 0,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.28031378984451294,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.104,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.2401,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.47,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.781,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000521400.6",
          "gene_symbol": "EPHX2",
          "hgnc_id": 3402,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.926G>A",
          "hgvs_p": "p.Cys309Tyr"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}