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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 8-91975938-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=8&pos=91975938&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "8",
      "pos": 91975938,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001198679.3",
      "consequences": [
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1T1",
          "gene_hgnc_id": 1535,
          "hgvs_c": "c.1315G>A",
          "hgvs_p": "p.Gly439Arg",
          "transcript": "NM_175634.3",
          "protein_id": "NP_783552.1",
          "transcript_support_level": null,
          "aa_start": 439,
          "aa_end": null,
          "aa_length": 604,
          "cds_start": 1315,
          "cds_end": null,
          "cds_length": 1815,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000523629.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_175634.3"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1T1",
          "gene_hgnc_id": 1535,
          "hgvs_c": "c.1315G>A",
          "hgvs_p": "p.Gly439Arg",
          "transcript": "ENST00000523629.7",
          "protein_id": "ENSP00000428543.1",
          "transcript_support_level": 5,
          "aa_start": 439,
          "aa_end": null,
          "aa_length": 604,
          "cds_start": 1315,
          "cds_end": null,
          "cds_length": 1815,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_175634.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000523629.7"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1T1",
          "gene_hgnc_id": 1535,
          "hgvs_c": "c.1234G>A",
          "hgvs_p": "p.Gly412Arg",
          "transcript": "ENST00000396218.5",
          "protein_id": "ENSP00000379520.1",
          "transcript_support_level": 1,
          "aa_start": 412,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 1234,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000396218.5"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1T1",
          "gene_hgnc_id": 1535,
          "hgvs_c": "c.1234G>A",
          "hgvs_p": "p.Gly412Arg",
          "transcript": "ENST00000518844.5",
          "protein_id": "ENSP00000430728.1",
          "transcript_support_level": 1,
          "aa_start": 412,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 1234,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000518844.5"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1T1",
          "gene_hgnc_id": 1535,
          "hgvs_c": "c.1204G>A",
          "hgvs_p": "p.Gly402Arg",
          "transcript": "ENST00000360348.6",
          "protein_id": "ENSP00000353504.2",
          "transcript_support_level": 1,
          "aa_start": 402,
          "aa_end": null,
          "aa_length": 567,
          "cds_start": 1204,
          "cds_end": null,
          "cds_length": 1704,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000360348.6"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1T1",
          "gene_hgnc_id": 1535,
          "hgvs_c": "c.1204G>A",
          "hgvs_p": "p.Gly402Arg",
          "transcript": "ENST00000422361.6",
          "protein_id": "ENSP00000390137.2",
          "transcript_support_level": 1,
          "aa_start": 402,
          "aa_end": null,
          "aa_length": 567,
          "cds_start": 1204,
          "cds_end": null,
          "cds_length": 1704,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000422361.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1T1",
          "gene_hgnc_id": 1535,
          "hgvs_c": "n.*186G>A",
          "hgvs_p": null,
          "transcript": "ENST00000521751.5",
          "protein_id": "ENSP00000430778.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000521751.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1T1",
          "gene_hgnc_id": 1535,
          "hgvs_c": "n.*186G>A",
          "hgvs_p": null,
          "transcript": "ENST00000521751.5",
          "protein_id": "ENSP00000430778.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000521751.5"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1T1",
          "gene_hgnc_id": 1535,
          "hgvs_c": "c.1492G>A",
          "hgvs_p": "p.Gly498Arg",
          "transcript": "NM_001198679.3",
          "protein_id": "NP_001185608.1",
          "transcript_support_level": null,
          "aa_start": 498,
          "aa_end": null,
          "aa_length": 663,
          "cds_start": 1492,
          "cds_end": null,
          "cds_length": 1992,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001198679.3"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1T1",
          "gene_hgnc_id": 1535,
          "hgvs_c": "c.1492G>A",
          "hgvs_p": "p.Gly498Arg",
          "transcript": "ENST00000436581.7",
          "protein_id": "ENSP00000402257.3",
          "transcript_support_level": 5,
          "aa_start": 498,
          "aa_end": null,
          "aa_length": 663,
          "cds_start": 1492,
          "cds_end": null,
          "cds_length": 1992,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000436581.7"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1T1",
          "gene_hgnc_id": 1535,
          "hgvs_c": "c.1399G>A",
          "hgvs_p": "p.Gly467Arg",
          "transcript": "NM_001395209.1",
          "protein_id": "NP_001382138.1",
          "transcript_support_level": null,
          "aa_start": 467,
          "aa_end": null,
          "aa_length": 632,
          "cds_start": 1399,
          "cds_end": null,
          "cds_length": 1899,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001395209.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1T1",
          "gene_hgnc_id": 1535,
          "hgvs_c": "c.1348G>A",
          "hgvs_p": "p.Gly450Arg",
          "transcript": "NM_001198634.2",
          "protein_id": "NP_001185563.1",
          "transcript_support_level": null,
          "aa_start": 450,
          "aa_end": null,
          "aa_length": 615,
          "cds_start": 1348,
          "cds_end": null,
          "cds_length": 1848,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001198634.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1T1",
          "gene_hgnc_id": 1535,
          "hgvs_c": "c.1348G>A",
          "hgvs_p": "p.Gly450Arg",
          "transcript": "ENST00000520724.5",
          "protein_id": "ENSP00000428742.2",
          "transcript_support_level": 2,
          "aa_start": 450,
          "aa_end": null,
          "aa_length": 615,
          "cds_start": 1348,
          "cds_end": null,
          "cds_length": 1848,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000520724.5"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1T1",
          "gene_hgnc_id": 1535,
          "hgvs_c": "c.1315G>A",
          "hgvs_p": "p.Gly439Arg",
          "transcript": "NM_001198626.2",
          "protein_id": "NP_001185555.1",
          "transcript_support_level": null,
          "aa_start": 439,
          "aa_end": null,
          "aa_length": 604,
          "cds_start": 1315,
          "cds_end": null,
          "cds_length": 1815,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001198626.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1T1",
          "gene_hgnc_id": 1535,
          "hgvs_c": "c.1315G>A",
          "hgvs_p": "p.Gly439Arg",
          "transcript": "NM_001198627.2",
          "protein_id": "NP_001185556.1",
          "transcript_support_level": null,
          "aa_start": 439,
          "aa_end": null,
          "aa_length": 604,
          "cds_start": 1315,
          "cds_end": null,
          "cds_length": 1815,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001198627.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1T1",
          "gene_hgnc_id": 1535,
          "hgvs_c": "c.1315G>A",
          "hgvs_p": "p.Gly439Arg",
          "transcript": "NM_001198628.2",
          "protein_id": "NP_001185557.1",
          "transcript_support_level": null,
          "aa_start": 439,
          "aa_end": null,
          "aa_length": 604,
          "cds_start": 1315,
          "cds_end": null,
          "cds_length": 1815,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001198628.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1T1",
          "gene_hgnc_id": 1535,
          "hgvs_c": "c.1315G>A",
          "hgvs_p": "p.Gly439Arg",
          "transcript": "NM_001198629.2",
          "protein_id": "NP_001185558.1",
          "transcript_support_level": null,
          "aa_start": 439,
          "aa_end": null,
          "aa_length": 604,
          "cds_start": 1315,
          "cds_end": null,
          "cds_length": 1815,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001198629.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1T1",
          "gene_hgnc_id": 1535,
          "hgvs_c": "c.1315G>A",
          "hgvs_p": "p.Gly439Arg",
          "transcript": "NM_001198630.2",
          "protein_id": "NP_001185559.1",
          "transcript_support_level": null,
          "aa_start": 439,
          "aa_end": null,
          "aa_length": 604,
          "cds_start": 1315,
          "cds_end": null,
          "cds_length": 1815,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001198630.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1T1",
          "gene_hgnc_id": 1535,
          "hgvs_c": "c.1315G>A",
          "hgvs_p": "p.Gly439Arg",
          "transcript": "NM_001198631.2",
          "protein_id": "NP_001185560.1",
          "transcript_support_level": null,
          "aa_start": 439,
          "aa_end": null,
          "aa_length": 604,
          "cds_start": 1315,
          "cds_end": null,
          "cds_length": 1815,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001198631.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RUNX1T1",
          "gene_hgnc_id": 1535,
          "hgvs_c": "c.1315G>A",
          "hgvs_p": "p.Gly439Arg",
          "transcript": "ENST00000517919.6",
          "protein_id": "ENSP00000429506.2",
          "transcript_support_level": 4,
          "aa_start": 439,
          "aa_end": null,
          "aa_length": 604,
          "cds_start": 1315,
          "cds_end": null,
          "cds_length": 1815,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
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        {
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          "biotype": "nonsense_mediated_decay",
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      ],
      "gene_symbol": "RUNX1T1",
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      "dbsnp": null,
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8445258140563965,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.62,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.8561,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.14,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.849,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 4,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3_Moderate",
      "acmg_by_gene": [
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001198679.3",
          "gene_symbol": "RUNX1T1",
          "hgnc_id": 1535,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1492G>A",
          "hgvs_p": "p.Gly498Arg"
        },
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000522980.1",
          "gene_symbol": "ENSG00000253576",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.31C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "RUNX1-IT1 related disorder",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "RUNX1-IT1 related disorder",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}