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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 8-99965339-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=8&pos=99965339&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "8",
      "pos": 99965339,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_015668.5",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RGS22",
          "gene_hgnc_id": 24499,
          "hgvs_c": "c.3611G>A",
          "hgvs_p": "p.Arg1204Gln",
          "transcript": "NM_015668.5",
          "protein_id": "NP_056483.3",
          "transcript_support_level": null,
          "aa_start": 1204,
          "aa_end": null,
          "aa_length": 1264,
          "cds_start": 3611,
          "cds_end": null,
          "cds_length": 3795,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000360863.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_015668.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RGS22",
          "gene_hgnc_id": 24499,
          "hgvs_c": "c.3611G>A",
          "hgvs_p": "p.Arg1204Gln",
          "transcript": "ENST00000360863.11",
          "protein_id": "ENSP00000354109.6",
          "transcript_support_level": 1,
          "aa_start": 1204,
          "aa_end": null,
          "aa_length": 1264,
          "cds_start": 3611,
          "cds_end": null,
          "cds_length": 3795,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_015668.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000360863.11"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RGS22",
          "gene_hgnc_id": 24499,
          "hgvs_c": "c.3575G>A",
          "hgvs_p": "p.Arg1192Gln",
          "transcript": "ENST00000523437.5",
          "protein_id": "ENSP00000428212.1",
          "transcript_support_level": 1,
          "aa_start": 1192,
          "aa_end": null,
          "aa_length": 1252,
          "cds_start": 3575,
          "cds_end": null,
          "cds_length": 3759,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000523437.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RGS22",
          "gene_hgnc_id": 24499,
          "hgvs_c": "c.3575G>A",
          "hgvs_p": "p.Arg1192Gln",
          "transcript": "NM_001286692.2",
          "protein_id": "NP_001273621.1",
          "transcript_support_level": null,
          "aa_start": 1192,
          "aa_end": null,
          "aa_length": 1252,
          "cds_start": 3575,
          "cds_end": null,
          "cds_length": 3759,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001286692.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RGS22",
          "gene_hgnc_id": 24499,
          "hgvs_c": "c.3452G>A",
          "hgvs_p": "p.Arg1151Gln",
          "transcript": "ENST00000870307.1",
          "protein_id": "ENSP00000540366.1",
          "transcript_support_level": null,
          "aa_start": 1151,
          "aa_end": null,
          "aa_length": 1211,
          "cds_start": 3452,
          "cds_end": null,
          "cds_length": 3636,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000870307.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RGS22",
          "gene_hgnc_id": 24499,
          "hgvs_c": "c.3068G>A",
          "hgvs_p": "p.Arg1023Gln",
          "transcript": "NM_001286693.2",
          "protein_id": "NP_001273622.1",
          "transcript_support_level": null,
          "aa_start": 1023,
          "aa_end": null,
          "aa_length": 1083,
          "cds_start": 3068,
          "cds_end": null,
          "cds_length": 3252,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001286693.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RGS22",
          "gene_hgnc_id": 24499,
          "hgvs_c": "c.3068G>A",
          "hgvs_p": "p.Arg1023Gln",
          "transcript": "ENST00000523287.5",
          "protein_id": "ENSP00000429382.1",
          "transcript_support_level": 2,
          "aa_start": 1023,
          "aa_end": null,
          "aa_length": 1083,
          "cds_start": 3068,
          "cds_end": null,
          "cds_length": 3252,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000523287.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RGS22",
          "gene_hgnc_id": 24499,
          "hgvs_c": "c.2039G>A",
          "hgvs_p": "p.Arg680Gln",
          "transcript": "ENST00000955932.1",
          "protein_id": "ENSP00000625991.1",
          "transcript_support_level": null,
          "aa_start": 680,
          "aa_end": null,
          "aa_length": 740,
          "cds_start": 2039,
          "cds_end": null,
          "cds_length": 2223,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000955932.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RGS22",
          "gene_hgnc_id": 24499,
          "hgvs_c": "c.227G>A",
          "hgvs_p": "p.Arg76Gln",
          "transcript": "ENST00000517843.5",
          "protein_id": "ENSP00000430378.1",
          "transcript_support_level": 3,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 111,
          "cds_start": 227,
          "cds_end": null,
          "cds_length": 336,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000517843.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RGS22",
          "gene_hgnc_id": 24499,
          "hgvs_c": "c.3452G>A",
          "hgvs_p": "p.Arg1151Gln",
          "transcript": "XM_005250856.4",
          "protein_id": "XP_005250913.1",
          "transcript_support_level": null,
          "aa_start": 1151,
          "aa_end": null,
          "aa_length": 1211,
          "cds_start": 3452,
          "cds_end": null,
          "cds_length": 3636,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005250856.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RGS22",
          "gene_hgnc_id": 24499,
          "hgvs_c": "c.3452G>A",
          "hgvs_p": "p.Arg1151Gln",
          "transcript": "XM_005250857.4",
          "protein_id": "XP_005250914.1",
          "transcript_support_level": null,
          "aa_start": 1151,
          "aa_end": null,
          "aa_length": 1211,
          "cds_start": 3452,
          "cds_end": null,
          "cds_length": 3636,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005250857.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RGS22",
          "gene_hgnc_id": 24499,
          "hgvs_c": "c.3449G>A",
          "hgvs_p": "p.Arg1150Gln",
          "transcript": "XM_005250858.4",
          "protein_id": "XP_005250915.1",
          "transcript_support_level": null,
          "aa_start": 1150,
          "aa_end": null,
          "aa_length": 1210,
          "cds_start": 3449,
          "cds_end": null,
          "cds_length": 3633,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005250858.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RGS22",
          "gene_hgnc_id": 24499,
          "hgvs_c": "c.3323G>A",
          "hgvs_p": "p.Arg1108Gln",
          "transcript": "XM_047421679.1",
          "protein_id": "XP_047277635.1",
          "transcript_support_level": null,
          "aa_start": 1108,
          "aa_end": null,
          "aa_length": 1168,
          "cds_start": 3323,
          "cds_end": null,
          "cds_length": 3507,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047421679.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RGS22",
          "gene_hgnc_id": 24499,
          "hgvs_c": "c.3293G>A",
          "hgvs_p": "p.Arg1098Gln",
          "transcript": "XM_017013309.3",
          "protein_id": "XP_016868798.1",
          "transcript_support_level": null,
          "aa_start": 1098,
          "aa_end": null,
          "aa_length": 1158,
          "cds_start": 3293,
          "cds_end": null,
          "cds_length": 3477,
          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "protein_coding",
          "feature": "XM_017013309.3"
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RGS22",
          "gene_hgnc_id": 24499,
          "hgvs_c": "c.3290G>A",
          "hgvs_p": "p.Arg1097Gln",
          "transcript": "XM_017013310.3",
          "protein_id": "XP_016868799.1",
          "transcript_support_level": null,
          "aa_start": 1097,
          "aa_end": null,
          "aa_length": 1157,
          "cds_start": 3290,
          "cds_end": null,
          "cds_length": 3474,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017013310.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RGS22",
          "gene_hgnc_id": 24499,
          "hgvs_c": "c.3290G>A",
          "hgvs_p": "p.Arg1097Gln",
          "transcript": "XM_047421681.1",
          "protein_id": "XP_047277637.1",
          "transcript_support_level": null,
          "aa_start": 1097,
          "aa_end": null,
          "aa_length": 1157,
          "cds_start": 3290,
          "cds_end": null,
          "cds_length": 3474,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047421681.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RGS22",
          "gene_hgnc_id": 24499,
          "hgvs_c": "c.2246G>A",
          "hgvs_p": "p.Arg749Gln",
          "transcript": "XM_017013311.2",
          "protein_id": "XP_016868800.1",
          "transcript_support_level": null,
          "aa_start": 749,
          "aa_end": null,
          "aa_length": 809,
          "cds_start": 2246,
          "cds_end": null,
          "cds_length": 2430,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_017013311.2"
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RGS22",
          "gene_hgnc_id": 24499,
          "hgvs_c": "c.1778G>A",
          "hgvs_p": "p.Arg593Gln",
          "transcript": "XM_024447121.2",
          "protein_id": "XP_024302889.1",
          "transcript_support_level": null,
          "aa_start": 593,
          "aa_end": null,
          "aa_length": 653,
          "cds_start": 1778,
          "cds_end": null,
          "cds_length": 1962,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_024447121.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RGS22",
          "gene_hgnc_id": 24499,
          "hgvs_c": "n.1839G>A",
          "hgvs_p": null,
          "transcript": "ENST00000517769.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000517769.5"
        }
      ],
      "gene_symbol": "RGS22",
      "gene_hgnc_id": 24499,
      "dbsnp": "rs760451465",
      "frequency_reference_population": 0.00001986857,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 32,
      "gnomad_exomes_af": 0.0000212557,
      "gnomad_genomes_af": 0.00000657237,
      "gnomad_exomes_ac": 31,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.13429978489875793,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.024,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0992,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.54,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.026,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_015668.5",
          "gene_symbol": "RGS22",
          "hgnc_id": 24499,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.3611G>A",
          "hgvs_p": "p.Arg1204Gln"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}