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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 9-124999958-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=9&pos=124999958&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "9",
      "pos": 124999958,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_173690.5",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCAI",
          "gene_hgnc_id": 26709,
          "hgvs_c": "c.1177A>G",
          "hgvs_p": "p.Ser393Gly",
          "transcript": "NM_001144877.3",
          "protein_id": "NP_001138349.1",
          "transcript_support_level": null,
          "aa_start": 393,
          "aa_end": null,
          "aa_length": 606,
          "cds_start": 1177,
          "cds_end": null,
          "cds_length": 1821,
          "cdna_start": 1268,
          "cdna_end": null,
          "cdna_length": 12111,
          "mane_select": "ENST00000336505.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001144877.3"
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCAI",
          "gene_hgnc_id": 26709,
          "hgvs_c": "c.1177A>G",
          "hgvs_p": "p.Ser393Gly",
          "transcript": "ENST00000336505.11",
          "protein_id": "ENSP00000336756.6",
          "transcript_support_level": 1,
          "aa_start": 393,
          "aa_end": null,
          "aa_length": 606,
          "cds_start": 1177,
          "cds_end": null,
          "cds_length": 1821,
          "cdna_start": 1268,
          "cdna_end": null,
          "cdna_length": 12111,
          "mane_select": "NM_001144877.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000336505.11"
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCAI",
          "gene_hgnc_id": 26709,
          "hgvs_c": "c.1246A>G",
          "hgvs_p": "p.Ser416Gly",
          "transcript": "ENST00000373549.8",
          "protein_id": "ENSP00000362650.4",
          "transcript_support_level": 1,
          "aa_start": 416,
          "aa_end": null,
          "aa_length": 629,
          "cds_start": 1246,
          "cds_end": null,
          "cds_length": 1890,
          "cdna_start": 1305,
          "cdna_end": null,
          "cdna_length": 1968,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000373549.8"
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCAI",
          "gene_hgnc_id": 26709,
          "hgvs_c": "c.1246A>G",
          "hgvs_p": "p.Ser416Gly",
          "transcript": "NM_173690.5",
          "protein_id": "NP_775961.2",
          "transcript_support_level": null,
          "aa_start": 416,
          "aa_end": null,
          "aa_length": 629,
          "cds_start": 1246,
          "cds_end": null,
          "cds_length": 1890,
          "cdna_start": 1337,
          "cdna_end": null,
          "cdna_length": 12180,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_173690.5"
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCAI",
          "gene_hgnc_id": 26709,
          "hgvs_c": "c.1159A>G",
          "hgvs_p": "p.Ser387Gly",
          "transcript": "ENST00000858987.1",
          "protein_id": "ENSP00000529046.1",
          "transcript_support_level": null,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 600,
          "cds_start": 1159,
          "cds_end": null,
          "cds_length": 1803,
          "cdna_start": 1242,
          "cdna_end": null,
          "cdna_length": 2030,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000858987.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCAI",
          "gene_hgnc_id": 26709,
          "hgvs_c": "c.1081A>G",
          "hgvs_p": "p.Ser361Gly",
          "transcript": "ENST00000858986.1",
          "protein_id": "ENSP00000529045.1",
          "transcript_support_level": null,
          "aa_start": 361,
          "aa_end": null,
          "aa_length": 574,
          "cds_start": 1081,
          "cds_end": null,
          "cds_length": 1725,
          "cdna_start": 1132,
          "cdna_end": null,
          "cdna_length": 2858,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000858986.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCAI",
          "gene_hgnc_id": 26709,
          "hgvs_c": "c.1045A>G",
          "hgvs_p": "p.Ser349Gly",
          "transcript": "ENST00000858985.1",
          "protein_id": "ENSP00000529044.1",
          "transcript_support_level": null,
          "aa_start": 349,
          "aa_end": null,
          "aa_length": 562,
          "cds_start": 1045,
          "cds_end": null,
          "cds_length": 1689,
          "cdna_start": 1119,
          "cdna_end": null,
          "cdna_length": 3073,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000858985.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCAI",
          "gene_hgnc_id": 26709,
          "hgvs_c": "c.994A>G",
          "hgvs_p": "p.Ser332Gly",
          "transcript": "ENST00000923997.1",
          "protein_id": "ENSP00000594056.1",
          "transcript_support_level": null,
          "aa_start": 332,
          "aa_end": null,
          "aa_length": 545,
          "cds_start": 994,
          "cds_end": null,
          "cds_length": 1638,
          "cdna_start": 1007,
          "cdna_end": null,
          "cdna_length": 6624,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923997.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCAI",
          "gene_hgnc_id": 26709,
          "hgvs_c": "c.991A>G",
          "hgvs_p": "p.Ser331Gly",
          "transcript": "ENST00000952247.1",
          "protein_id": "ENSP00000622306.1",
          "transcript_support_level": null,
          "aa_start": 331,
          "aa_end": null,
          "aa_length": 544,
          "cds_start": 991,
          "cds_end": null,
          "cds_length": 1635,
          "cdna_start": 1082,
          "cdna_end": null,
          "cdna_length": 2368,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952247.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCAI",
          "gene_hgnc_id": 26709,
          "hgvs_c": "n.1177A>G",
          "hgvs_p": null,
          "transcript": "ENST00000477186.5",
          "protein_id": "ENSP00000419576.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1808,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000477186.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCAI",
          "gene_hgnc_id": 26709,
          "hgvs_c": "n.436A>G",
          "hgvs_p": null,
          "transcript": "ENST00000487795.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 483,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000487795.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCAI",
          "gene_hgnc_id": 26709,
          "hgvs_c": "n.1337A>G",
          "hgvs_p": null,
          "transcript": "XR_007061281.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1784,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "XR_007061281.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCAI",
          "gene_hgnc_id": 26709,
          "hgvs_c": "n.1268A>G",
          "hgvs_p": null,
          "transcript": "XR_007061282.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1715,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "XR_007061282.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCAI",
          "gene_hgnc_id": 26709,
          "hgvs_c": "n.1268A>G",
          "hgvs_p": null,
          "transcript": "XR_007061283.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1632,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "XR_007061283.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCAI",
          "gene_hgnc_id": 26709,
          "hgvs_c": "n.1337A>G",
          "hgvs_p": null,
          "transcript": "XR_929768.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1701,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "XR_929768.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCAI",
          "gene_hgnc_id": 26709,
          "hgvs_c": "n.-36A>G",
          "hgvs_p": null,
          "transcript": "ENST00000467917.5",
          "protein_id": "ENSP00000418148.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 691,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000467917.5"
        }
      ],
      "gene_symbol": "SCAI",
      "gene_hgnc_id": 26709,
      "dbsnp": null,
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": 0,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 0,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.29476198554039,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.168,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0875,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.37,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 7.672,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_173690.5",
          "gene_symbol": "SCAI",
          "hgnc_id": 26709,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1246A>G",
          "hgvs_p": "p.Ser416Gly"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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