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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 9-131127590-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=9&pos=131127590&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "9",
      "pos": 131127590,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_005085.4",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP214",
          "gene_hgnc_id": 8064,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Arg38Cys",
          "transcript": "NM_005085.4",
          "protein_id": "NP_005076.3",
          "transcript_support_level": null,
          "aa_start": 38,
          "aa_end": null,
          "aa_length": 2090,
          "cds_start": 112,
          "cds_end": null,
          "cds_length": 6273,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000359428.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_005085.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP214",
          "gene_hgnc_id": 8064,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Arg38Cys",
          "transcript": "ENST00000359428.10",
          "protein_id": "ENSP00000352400.5",
          "transcript_support_level": 1,
          "aa_start": 38,
          "aa_end": null,
          "aa_length": 2090,
          "cds_start": 112,
          "cds_end": null,
          "cds_length": 6273,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_005085.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000359428.10"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP214",
          "gene_hgnc_id": 8064,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Arg38Cys",
          "transcript": "ENST00000411637.6",
          "protein_id": "ENSP00000396576.2",
          "transcript_support_level": 1,
          "aa_start": 38,
          "aa_end": null,
          "aa_length": 2080,
          "cds_start": 112,
          "cds_end": null,
          "cds_length": 6243,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000411637.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_premature_start_codon_gain_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP214",
          "gene_hgnc_id": 8064,
          "hgvs_c": "c.-159C>T",
          "hgvs_p": null,
          "transcript": "ENST00000651639.1",
          "protein_id": "ENSP00000498545.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 186,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 561,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000651639.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_premature_start_codon_gain_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP214",
          "gene_hgnc_id": 8064,
          "hgvs_c": "c.-159C>T",
          "hgvs_p": null,
          "transcript": "ENST00000531584.1",
          "protein_id": "ENSP00000435874.1",
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 51,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 156,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000531584.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP214",
          "gene_hgnc_id": 8064,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Arg38Cys",
          "transcript": "ENST00000897205.1",
          "protein_id": "ENSP00000567264.1",
          "transcript_support_level": null,
          "aa_start": 38,
          "aa_end": null,
          "aa_length": 2091,
          "cds_start": 112,
          "cds_end": null,
          "cds_length": 6276,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897205.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP214",
          "gene_hgnc_id": 8064,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Arg38Cys",
          "transcript": "NM_001318324.2",
          "protein_id": "NP_001305253.1",
          "transcript_support_level": null,
          "aa_start": 38,
          "aa_end": null,
          "aa_length": 2080,
          "cds_start": 112,
          "cds_end": null,
          "cds_length": 6243,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318324.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP214",
          "gene_hgnc_id": 8064,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Arg38Cys",
          "transcript": "ENST00000897208.1",
          "protein_id": "ENSP00000567267.1",
          "transcript_support_level": null,
          "aa_start": 38,
          "aa_end": null,
          "aa_length": 2079,
          "cds_start": 112,
          "cds_end": null,
          "cds_length": 6240,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897208.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP214",
          "gene_hgnc_id": 8064,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Arg38Cys",
          "transcript": "ENST00000897209.1",
          "protein_id": "ENSP00000567268.1",
          "transcript_support_level": null,
          "aa_start": 38,
          "aa_end": null,
          "aa_length": 2079,
          "cds_start": 112,
          "cds_end": null,
          "cds_length": 6240,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897209.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP214",
          "gene_hgnc_id": 8064,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Arg38Cys",
          "transcript": "ENST00000652454.1",
          "protein_id": "ENSP00000499001.1",
          "transcript_support_level": null,
          "aa_start": 38,
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          "aa_length": 2078,
          "cds_start": 112,
          "cds_end": null,
          "cds_length": 6237,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000652454.1"
        },
        {
          "aa_ref": "R",
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          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP214",
          "gene_hgnc_id": 8064,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Arg38Cys",
          "transcript": "ENST00000922158.1",
          "protein_id": "ENSP00000592217.1",
          "transcript_support_level": null,
          "aa_start": 38,
          "aa_end": null,
          "aa_length": 2042,
          "cds_start": 112,
          "cds_end": null,
          "cds_length": 6129,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
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        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": true,
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          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
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          "gene_symbol": "NUP214",
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          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Arg38Cys",
          "transcript": "ENST00000897206.1",
          "protein_id": "ENSP00000567265.1",
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          "cdna_start": null,
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          "mane_select": null,
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        {
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          "protein_coding": true,
          "strand": true,
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            "missense_variant"
          ],
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          "intron_rank": null,
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          "gene_symbol": "NUP214",
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          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Arg38Cys",
          "transcript": "ENST00000922160.1",
          "protein_id": "ENSP00000592219.1",
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          "cds_start": 112,
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          "cdna_start": null,
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        {
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          ],
          "exon_rank": 2,
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          "exon_count": 34,
          "intron_rank": null,
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          "gene_symbol": "NUP214",
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          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Arg38Cys",
          "transcript": "ENST00000922161.1",
          "protein_id": "ENSP00000592220.1",
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          "gene_symbol": "NUP214",
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          "hgvs_c": "c.112C>T",
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          "transcript": "ENST00000922162.1",
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          "cds_start": 112,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 2,
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          "exon_count": 33,
          "intron_rank": null,
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          "gene_symbol": "NUP214",
          "gene_hgnc_id": 8064,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Arg38Cys",
          "transcript": "ENST00000897207.1",
          "protein_id": "ENSP00000567266.1",
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          "biotype": "protein_coding",
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        {
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          "intron_rank": null,
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          "gene_symbol": "NUP214",
          "gene_hgnc_id": 8064,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Arg38Cys",
          "transcript": "ENST00000897210.1",
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        {
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          "intron_rank": null,
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          "gene_symbol": "NUP214",
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          "hgvs_c": "c.112C>T",
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        {
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          "gene_symbol": "NUP214",
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          "transcript": "ENST00000922164.1",
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          "biotype": "protein_coding",
          "feature": "ENST00000922164.1"
        },
        {
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          "protein_coding": true,
          "strand": true,
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          ],
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          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP214",
          "gene_hgnc_id": 8064,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Arg38Cys",
          "transcript": "ENST00000922163.1",
          "protein_id": "ENSP00000592222.1",
          "transcript_support_level": null,
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          "mane_select": null,
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      "dbsnp": "rs143595616",
      "frequency_reference_population": 0.00004337255,
      "hom_count_reference_population": 0,
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      "gnomad_exomes_af": 0.0000444659,
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      "gnomad_exomes_ac": 65,
      "gnomad_genomes_ac": 5,
      "gnomad_exomes_homalt": 0,
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      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8103669881820679,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.663,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.4327,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.33,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 4.06,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
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      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
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      "acmg_score": 11,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PM2,PP3,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 11,
          "benign_score": 0,
          "pathogenic_score": 11,
          "criteria": [
            "PM2",
            "PP3",
            "PP5_Very_Strong"
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          "verdict": "Pathogenic",
          "transcript": "NM_005085.4",
          "gene_symbol": "NUP214",
          "hgnc_id": 8064,
          "effects": [
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          "inheritance_mode": "AR",
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Arg38Cys"
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      ],
      "clinvar_disease": " 9, acute, infection-induced, susceptibility to,Encephalopathy,Progressive microcephaly,Recurrent encephalopathy",
      "clinvar_classification": "Pathogenic/Likely pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:1 LP:1",
      "phenotype_combined": "Progressive microcephaly;Recurrent encephalopathy|Encephalopathy, acute, infection-induced, susceptibility to, 9",
      "pathogenicity_classification_combined": "Pathogenic/Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}