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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 9-131523065-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=9&pos=131523065&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "9",
      "pos": 131523065,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "ENST00000402686.8",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT1",
          "gene_hgnc_id": 9202,
          "hgvs_c": "c.2137C>T",
          "hgvs_p": "p.Arg713Cys",
          "transcript": "NM_001077365.2",
          "protein_id": "NP_001070833.1",
          "transcript_support_level": null,
          "aa_start": 713,
          "aa_end": null,
          "aa_length": 725,
          "cds_start": 2137,
          "cds_end": null,
          "cds_length": 2178,
          "cdna_start": 2323,
          "cdna_end": null,
          "cdna_length": 3057,
          "mane_select": "ENST00000402686.8",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT1",
          "gene_hgnc_id": 9202,
          "hgvs_c": "c.2137C>T",
          "hgvs_p": "p.Arg713Cys",
          "transcript": "ENST00000402686.8",
          "protein_id": "ENSP00000385797.4",
          "transcript_support_level": 1,
          "aa_start": 713,
          "aa_end": null,
          "aa_length": 725,
          "cds_start": 2137,
          "cds_end": null,
          "cds_length": 2178,
          "cdna_start": 2323,
          "cdna_end": null,
          "cdna_length": 3057,
          "mane_select": "NM_001077365.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT1",
          "gene_hgnc_id": 9202,
          "hgvs_c": "c.2203C>T",
          "hgvs_p": "p.Arg735Cys",
          "transcript": "ENST00000372228.9",
          "protein_id": "ENSP00000361302.3",
          "transcript_support_level": 1,
          "aa_start": 735,
          "aa_end": null,
          "aa_length": 747,
          "cds_start": 2203,
          "cds_end": null,
          "cds_length": 2244,
          "cdna_start": 2518,
          "cdna_end": null,
          "cdna_length": 3252,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT1",
          "gene_hgnc_id": 9202,
          "hgvs_c": "c.2194C>T",
          "hgvs_p": "p.Arg732Cys",
          "transcript": "ENST00000423007.6",
          "protein_id": "ENSP00000404119.2",
          "transcript_support_level": 1,
          "aa_start": 732,
          "aa_end": null,
          "aa_length": 744,
          "cds_start": 2194,
          "cds_end": null,
          "cds_length": 2235,
          "cdna_start": 2194,
          "cdna_end": null,
          "cdna_length": 2235,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT1",
          "gene_hgnc_id": 9202,
          "hgvs_c": "c.2203C>T",
          "hgvs_p": "p.Arg735Cys",
          "transcript": "NM_001353193.2",
          "protein_id": "NP_001340122.2",
          "transcript_support_level": null,
          "aa_start": 735,
          "aa_end": null,
          "aa_length": 747,
          "cds_start": 2203,
          "cds_end": null,
          "cds_length": 2244,
          "cdna_start": 2629,
          "cdna_end": null,
          "cdna_length": 3363,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT1",
          "gene_hgnc_id": 9202,
          "hgvs_c": "c.2203C>T",
          "hgvs_p": "p.Arg735Cys",
          "transcript": "NM_007171.4",
          "protein_id": "NP_009102.4",
          "transcript_support_level": null,
          "aa_start": 735,
          "aa_end": null,
          "aa_length": 747,
          "cds_start": 2203,
          "cds_end": null,
          "cds_length": 2244,
          "cdna_start": 2389,
          "cdna_end": null,
          "cdna_length": 3123,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT1",
          "gene_hgnc_id": 9202,
          "hgvs_c": "c.2137C>T",
          "hgvs_p": "p.Arg713Cys",
          "transcript": "NM_001136113.2",
          "protein_id": "NP_001129585.1",
          "transcript_support_level": null,
          "aa_start": 713,
          "aa_end": null,
          "aa_length": 725,
          "cds_start": 2137,
          "cds_end": null,
          "cds_length": 2178,
          "cdna_start": 2563,
          "cdna_end": null,
          "cdna_length": 3297,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT1",
          "gene_hgnc_id": 9202,
          "hgvs_c": "c.2137C>T",
          "hgvs_p": "p.Arg713Cys",
          "transcript": "ENST00000676640.1",
          "protein_id": "ENSP00000503281.1",
          "transcript_support_level": null,
          "aa_start": 713,
          "aa_end": null,
          "aa_length": 725,
          "cds_start": 2137,
          "cds_end": null,
          "cds_length": 2178,
          "cdna_start": 2575,
          "cdna_end": null,
          "cdna_length": 3309,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT1",
          "gene_hgnc_id": 9202,
          "hgvs_c": "c.2125C>T",
          "hgvs_p": "p.Arg709Cys",
          "transcript": "NM_001374689.1",
          "protein_id": "NP_001361618.1",
          "transcript_support_level": null,
          "aa_start": 709,
          "aa_end": null,
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          "cds_start": 2125,
          "cds_end": null,
          "cds_length": 2166,
          "cdna_start": 2321,
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          "mane_select": null,
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        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
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          "gene_symbol": "POMT1",
          "gene_hgnc_id": 9202,
          "hgvs_c": "c.2047C>T",
          "hgvs_p": "p.Arg683Cys",
          "transcript": "NM_001353196.2",
          "protein_id": "NP_001340125.1",
          "transcript_support_level": null,
          "aa_start": 683,
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          "aa_length": 695,
          "cds_start": 2047,
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        {
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          "gene_symbol": "POMT1",
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          "hgvs_c": "c.2047C>T",
          "hgvs_p": "p.Arg683Cys",
          "transcript": "ENST00000678303.1",
          "protein_id": "ENSP00000503696.1",
          "transcript_support_level": null,
          "aa_start": 683,
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        {
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          "intron_rank": null,
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          "gene_symbol": "POMT1",
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          "hgvs_c": "c.2041C>T",
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        {
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          "hgvs_c": "c.2041C>T",
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        {
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          "gene_hgnc_id": 9202,
          "hgvs_c": "c.1975C>T",
          "hgvs_p": "p.Arg659Cys",
          "transcript": "NM_001077366.2",
          "protein_id": "NP_001070834.1",
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        {
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          "gene_symbol": "POMT1",
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        {
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          "gene_symbol": "POMT1",
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          "hgvs_c": "c.1918C>T",
          "hgvs_p": "p.Arg640Cys",
          "transcript": "NM_001374690.1",
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          "gene_symbol": "POMT1",
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          "hgvs_c": "c.1786C>T",
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          "transcript": "NM_001353195.2",
          "protein_id": "NP_001340124.1",
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        {
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      ],
      "gene_symbol": "POMT1",
      "gene_hgnc_id": 9202,
      "dbsnp": "rs147266709",
      "frequency_reference_population": 0.012752525,
      "hom_count_reference_population": 192,
      "allele_count_reference_population": 20551,
      "gnomad_exomes_af": 0.0130851,
      "gnomad_genomes_af": 0.00956627,
      "gnomad_exomes_ac": 19094,
      "gnomad_genomes_ac": 1457,
      "gnomad_exomes_homalt": 178,
      "gnomad_genomes_homalt": 14,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.016078084707260132,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.743,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.1938,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.07,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 3.723,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -20,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BS1,BS2",
      "acmg_by_gene": [
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BS1",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000402686.8",
          "gene_symbol": "POMT1",
          "hgnc_id": 9202,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2137C>T",
          "hgvs_p": "p.Arg713Cys"
        }
      ],
      "clinvar_disease": " type B1,Autosomal recessive limb-girdle muscular dystrophy type 2K,Muscular dystrophy-dystroglycanopathy (congenital with intellectual disability),Walker-Warburg congenital muscular dystrophy,not provided,not specified",
      "clinvar_classification": "Benign/Likely benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LB:4 B:4",
      "phenotype_combined": "not specified|Muscular dystrophy-dystroglycanopathy (congenital with intellectual disability), type B1;Walker-Warburg congenital muscular dystrophy;Autosomal recessive limb-girdle muscular dystrophy type 2K|not provided|Autosomal recessive limb-girdle muscular dystrophy type 2K",
      "pathogenicity_classification_combined": "Benign/Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}