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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 9-135779438-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=9&pos=135779438&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "9",
      "pos": 135779438,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000371757.7",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.2809A>G",
          "hgvs_p": "p.Ser937Gly",
          "transcript": "NM_020822.3",
          "protein_id": "NP_065873.2",
          "transcript_support_level": null,
          "aa_start": 937,
          "aa_end": null,
          "aa_length": 1235,
          "cds_start": 2809,
          "cds_end": null,
          "cds_length": 3708,
          "cdna_start": 2883,
          "cdna_end": null,
          "cdna_length": 7123,
          "mane_select": "ENST00000371757.7",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.2809A>G",
          "hgvs_p": "p.Ser937Gly",
          "transcript": "ENST00000371757.7",
          "protein_id": "ENSP00000360822.2",
          "transcript_support_level": 1,
          "aa_start": 937,
          "aa_end": null,
          "aa_length": 1235,
          "cds_start": 2809,
          "cds_end": null,
          "cds_length": 3708,
          "cdna_start": 2883,
          "cdna_end": null,
          "cdna_length": 7123,
          "mane_select": "NM_020822.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "n.*2419A>G",
          "hgvs_p": null,
          "transcript": "ENST00000460750.5",
          "protein_id": "ENSP00000418777.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4738,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "n.*2419A>G",
          "hgvs_p": null,
          "transcript": "ENST00000460750.5",
          "protein_id": "ENSP00000418777.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4738,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.2809A>G",
          "hgvs_p": "p.Ser937Gly",
          "transcript": "ENST00000487664.5",
          "protein_id": "ENSP00000417851.2",
          "transcript_support_level": 5,
          "aa_start": 937,
          "aa_end": null,
          "aa_length": 1256,
          "cds_start": 2809,
          "cds_end": null,
          "cds_length": 3771,
          "cdna_start": 2883,
          "cdna_end": null,
          "cdna_length": 3845,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.2752A>G",
          "hgvs_p": "p.Ser918Gly",
          "transcript": "ENST00000631073.2",
          "protein_id": "ENSP00000486130.1",
          "transcript_support_level": 5,
          "aa_start": 918,
          "aa_end": null,
          "aa_length": 1237,
          "cds_start": 2752,
          "cds_end": null,
          "cds_length": 3714,
          "cdna_start": 2760,
          "cdna_end": null,
          "cdna_length": 3948,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.2746A>G",
          "hgvs_p": "p.Ser916Gly",
          "transcript": "ENST00000490355.6",
          "protein_id": "ENSP00000418003.3",
          "transcript_support_level": 5,
          "aa_start": 916,
          "aa_end": null,
          "aa_length": 1235,
          "cds_start": 2746,
          "cds_end": null,
          "cds_length": 3708,
          "cdna_start": 2746,
          "cdna_end": null,
          "cdna_length": 3708,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.2752A>G",
          "hgvs_p": "p.Ser918Gly",
          "transcript": "ENST00000488444.6",
          "protein_id": "ENSP00000419007.3",
          "transcript_support_level": 5,
          "aa_start": 918,
          "aa_end": null,
          "aa_length": 1230,
          "cds_start": 2752,
          "cds_end": null,
          "cds_length": 3693,
          "cdna_start": 2755,
          "cdna_end": null,
          "cdna_length": 3696,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.2710A>G",
          "hgvs_p": "p.Ser904Gly",
          "transcript": "ENST00000263604.5",
          "protein_id": "ENSP00000263604.4",
          "transcript_support_level": 5,
          "aa_start": 904,
          "aa_end": null,
          "aa_length": 1223,
          "cds_start": 2710,
          "cds_end": null,
          "cds_length": 3672,
          "cdna_start": 2826,
          "cdna_end": null,
          "cdna_length": 5245,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.2692A>G",
          "hgvs_p": "p.Ser898Gly",
          "transcript": "ENST00000486577.6",
          "protein_id": "ENSP00000417578.3",
          "transcript_support_level": 5,
          "aa_start": 898,
          "aa_end": null,
          "aa_length": 1217,
          "cds_start": 2692,
          "cds_end": null,
          "cds_length": 3654,
          "cdna_start": 2798,
          "cdna_end": null,
          "cdna_length": 3760,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.2752A>G",
          "hgvs_p": "p.Ser918Gly",
          "transcript": "ENST00000491806.6",
          "protein_id": "ENSP00000419086.3",
          "transcript_support_level": 5,
          "aa_start": 918,
          "aa_end": null,
          "aa_length": 1216,
          "cds_start": 2752,
          "cds_end": null,
          "cds_length": 3651,
          "cdna_start": 2770,
          "cdna_end": null,
          "cdna_length": 3678,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.2674A>G",
          "hgvs_p": "p.Ser892Gly",
          "transcript": "NM_001272003.2",
          "protein_id": "NP_001258932.1",
          "transcript_support_level": null,
          "aa_start": 892,
          "aa_end": null,
          "aa_length": 1211,
          "cds_start": 2674,
          "cds_end": null,
          "cds_length": 3636,
          "cdna_start": 2748,
          "cdna_end": null,
          "cdna_length": 7051,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.2674A>G",
          "hgvs_p": "p.Ser892Gly",
          "transcript": "ENST00000628528.2",
          "protein_id": "ENSP00000486374.1",
          "transcript_support_level": 2,
          "aa_start": 892,
          "aa_end": null,
          "aa_length": 1211,
          "cds_start": 2674,
          "cds_end": null,
          "cds_length": 3636,
          "cdna_start": 2748,
          "cdna_end": null,
          "cdna_length": 7057,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.2650A>G",
          "hgvs_p": "p.Ser884Gly",
          "transcript": "ENST00000674572.1",
          "protein_id": "ENSP00000501742.1",
          "transcript_support_level": null,
          "aa_start": 884,
          "aa_end": null,
          "aa_length": 1203,
          "cds_start": 2650,
          "cds_end": null,
          "cds_length": 3612,
          "cdna_start": 2917,
          "cdna_end": null,
          "cdna_length": 4763,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.2644A>G",
          "hgvs_p": "p.Ser882Gly",
          "transcript": "ENST00000630792.2",
          "protein_id": "ENSP00000486486.1",
          "transcript_support_level": 5,
          "aa_start": 882,
          "aa_end": null,
          "aa_length": 1201,
          "cds_start": 2644,
          "cds_end": null,
          "cds_length": 3606,
          "cdna_start": 2644,
          "cdna_end": null,
          "cdna_length": 3606,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.2566A>G",
          "hgvs_p": "p.Ser856Gly",
          "transcript": "ENST00000676421.1",
          "protein_id": "ENSP00000502322.1",
          "transcript_support_level": null,
          "aa_start": 856,
          "aa_end": null,
          "aa_length": 1175,
          "cds_start": 2566,
          "cds_end": null,
          "cds_length": 3528,
          "cdna_start": 2650,
          "cdna_end": null,
          "cdna_length": 4598,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.2557A>G",
          "hgvs_p": "p.Ser853Gly",
          "transcript": "ENST00000675399.1",
          "protein_id": "ENSP00000501932.1",
          "transcript_support_level": null,
          "aa_start": 853,
          "aa_end": null,
          "aa_length": 1172,
          "cds_start": 2557,
          "cds_end": null,
          "cds_length": 3519,
          "cdna_start": 2647,
          "cdna_end": null,
          "cdna_length": 4493,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.2557A>G",
          "hgvs_p": "p.Ser853Gly",
          "transcript": "ENST00000675090.1",
          "protein_id": "ENSP00000501833.1",
          "transcript_support_level": null,
          "aa_start": 853,
          "aa_end": null,
          "aa_length": 1151,
          "cds_start": 2557,
          "cds_end": null,
          "cds_length": 3456,
          "cdna_start": 2659,
          "cdna_end": null,
          "cdna_length": 4442,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.2953A>G",
          "hgvs_p": "p.Ser985Gly",
          "transcript": "XM_011518878.4",
          "protein_id": "XP_011517180.1",
          "transcript_support_level": null,
          "aa_start": 985,
          "aa_end": null,
          "aa_length": 1283,
          "cds_start": 2953,
          "cds_end": null,
          "cds_length": 3852,
          "cdna_start": 2953,
          "cdna_end": null,
          "cdna_length": 7193,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.2944A>G",
          "hgvs_p": "p.Ser982Gly",
          "transcript": "XM_011518879.4",
          "protein_id": "XP_011517181.1",
          "transcript_support_level": null,
          "aa_start": 982,
          "aa_end": null,
          "aa_length": 1280,
          "cds_start": 2944,
          "cds_end": null,
          "cds_length": 3843,
          "cdna_start": 2944,
          "cdna_end": null,
          "cdna_length": 7184,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
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      "gene_symbol": "KCNT1",
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      "dbsnp": "rs1554778379",
      "frequency_reference_population": 6.8425663e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84257e-7,
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      "gnomad_exomes_homalt": 0,
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      "computational_score_selected": 0.2791205942630768,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.03999999910593033,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Benign",
      "alphamissense_score": 0.1025,
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      "bayesdelnoaf_score": -0.39,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 5.138,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.04,
      "spliceai_max_prediction": "Benign",
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      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM1,PM2,BP4,BP6",
      "acmg_by_gene": [
        {
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          "pathogenic_score": 4,
          "criteria": [
            "PM1",
            "PM2",
            "BP4",
            "BP6"
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          "verdict": "Uncertain_significance",
          "transcript": "ENST00000371757.7",
          "gene_symbol": "KCNT1",
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      "clinvar_disease": " 14,Autosomal dominant nocturnal frontal lobe epilepsy 5,Developmental and epileptic encephalopathy,not provided,not specified",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:2 LB:1",
      "phenotype_combined": "not specified|Developmental and epileptic encephalopathy, 14;Autosomal dominant nocturnal frontal lobe epilepsy 5|not provided",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}