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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 9-135792059-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=9&pos=135792059&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "9",
      "pos": 135792059,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_020822.3",
      "consequences": [
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.3606C>G",
          "hgvs_p": "p.Asp1202Glu",
          "transcript": "NM_020822.3",
          "protein_id": "NP_065873.2",
          "transcript_support_level": null,
          "aa_start": 1202,
          "aa_end": null,
          "aa_length": 1235,
          "cds_start": 3606,
          "cds_end": null,
          "cds_length": 3708,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000371757.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_020822.3"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.3606C>G",
          "hgvs_p": "p.Asp1202Glu",
          "transcript": "ENST00000371757.7",
          "protein_id": "ENSP00000360822.2",
          "transcript_support_level": 1,
          "aa_start": 1202,
          "aa_end": null,
          "aa_length": 1235,
          "cds_start": 3606,
          "cds_end": null,
          "cds_length": 3708,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_020822.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000371757.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 32,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "n.*3279C>G",
          "hgvs_p": null,
          "transcript": "ENST00000460750.5",
          "protein_id": "ENSP00000418777.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000460750.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 32,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "n.*3279C>G",
          "hgvs_p": null,
          "transcript": "ENST00000460750.5",
          "protein_id": "ENSP00000418777.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000460750.5"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 32,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.3669C>G",
          "hgvs_p": "p.Asp1223Glu",
          "transcript": "ENST00000487664.5",
          "protein_id": "ENSP00000417851.2",
          "transcript_support_level": 5,
          "aa_start": 1223,
          "aa_end": null,
          "aa_length": 1256,
          "cds_start": 3669,
          "cds_end": null,
          "cds_length": 3771,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000487664.5"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 32,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.3612C>G",
          "hgvs_p": "p.Asp1204Glu",
          "transcript": "ENST00000631073.2",
          "protein_id": "ENSP00000486130.1",
          "transcript_support_level": 5,
          "aa_start": 1204,
          "aa_end": null,
          "aa_length": 1237,
          "cds_start": 3612,
          "cds_end": null,
          "cds_length": 3714,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000631073.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 32,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.3606C>G",
          "hgvs_p": "p.Asp1202Glu",
          "transcript": "ENST00000490355.6",
          "protein_id": "ENSP00000418003.3",
          "transcript_support_level": 5,
          "aa_start": 1202,
          "aa_end": null,
          "aa_length": 1235,
          "cds_start": 3606,
          "cds_end": null,
          "cds_length": 3708,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000490355.6"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.3591C>G",
          "hgvs_p": "p.Asp1197Glu",
          "transcript": "ENST00000488444.6",
          "protein_id": "ENSP00000419007.3",
          "transcript_support_level": 5,
          "aa_start": 1197,
          "aa_end": null,
          "aa_length": 1230,
          "cds_start": 3591,
          "cds_end": null,
          "cds_length": 3693,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000488444.6"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.3570C>G",
          "hgvs_p": "p.Asp1190Glu",
          "transcript": "ENST00000263604.5",
          "protein_id": "ENSP00000263604.4",
          "transcript_support_level": 5,
          "aa_start": 1190,
          "aa_end": null,
          "aa_length": 1223,
          "cds_start": 3570,
          "cds_end": null,
          "cds_length": 3672,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000263604.5"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 32,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.3552C>G",
          "hgvs_p": "p.Asp1184Glu",
          "transcript": "ENST00000486577.6",
          "protein_id": "ENSP00000417578.3",
          "transcript_support_level": 5,
          "aa_start": 1184,
          "aa_end": null,
          "aa_length": 1217,
          "cds_start": 3552,
          "cds_end": null,
          "cds_length": 3654,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000486577.6"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.3549C>G",
          "hgvs_p": "p.Asp1183Glu",
          "transcript": "ENST00000491806.6",
          "protein_id": "ENSP00000419086.3",
          "transcript_support_level": 5,
          "aa_start": 1183,
          "aa_end": null,
          "aa_length": 1216,
          "cds_start": 3549,
          "cds_end": null,
          "cds_length": 3651,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000491806.6"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.3534C>G",
          "hgvs_p": "p.Asp1178Glu",
          "transcript": "NM_001272003.2",
          "protein_id": "NP_001258932.1",
          "transcript_support_level": null,
          "aa_start": 1178,
          "aa_end": null,
          "aa_length": 1211,
          "cds_start": 3534,
          "cds_end": null,
          "cds_length": 3636,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001272003.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.3534C>G",
          "hgvs_p": "p.Asp1178Glu",
          "transcript": "ENST00000628528.2",
          "protein_id": "ENSP00000486374.1",
          "transcript_support_level": 2,
          "aa_start": 1178,
          "aa_end": null,
          "aa_length": 1211,
          "cds_start": 3534,
          "cds_end": null,
          "cds_length": 3636,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000628528.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.3525C>G",
          "hgvs_p": "p.Asp1175Glu",
          "transcript": "ENST00000968545.1",
          "protein_id": "ENSP00000638604.1",
          "transcript_support_level": null,
          "aa_start": 1175,
          "aa_end": null,
          "aa_length": 1208,
          "cds_start": 3525,
          "cds_end": null,
          "cds_length": 3627,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968545.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 32,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.3510C>G",
          "hgvs_p": "p.Asp1170Glu",
          "transcript": "ENST00000674572.1",
          "protein_id": "ENSP00000501742.1",
          "transcript_support_level": null,
          "aa_start": 1170,
          "aa_end": null,
          "aa_length": 1203,
          "cds_start": 3510,
          "cds_end": null,
          "cds_length": 3612,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000674572.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.3504C>G",
          "hgvs_p": "p.Asp1168Glu",
          "transcript": "ENST00000630792.2",
          "protein_id": "ENSP00000486486.1",
          "transcript_support_level": 5,
          "aa_start": 1168,
          "aa_end": null,
          "aa_length": 1201,
          "cds_start": 3504,
          "cds_end": null,
          "cds_length": 3606,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000630792.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.3426C>G",
          "hgvs_p": "p.Asp1142Glu",
          "transcript": "ENST00000676421.1",
          "protein_id": "ENSP00000502322.1",
          "transcript_support_level": null,
          "aa_start": 1142,
          "aa_end": null,
          "aa_length": 1175,
          "cds_start": 3426,
          "cds_end": null,
          "cds_length": 3528,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000676421.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.3417C>G",
          "hgvs_p": "p.Asp1139Glu",
          "transcript": "ENST00000675399.1",
          "protein_id": "ENSP00000501932.1",
          "transcript_support_level": null,
          "aa_start": 1139,
          "aa_end": null,
          "aa_length": 1172,
          "cds_start": 3417,
          "cds_end": null,
          "cds_length": 3519,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000675399.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.3354C>G",
          "hgvs_p": "p.Asp1118Glu",
          "transcript": "ENST00000675090.1",
          "protein_id": "ENSP00000501833.1",
          "transcript_support_level": null,
          "aa_start": 1118,
          "aa_end": null,
          "aa_length": 1151,
          "cds_start": 3354,
          "cds_end": null,
          "cds_length": 3456,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000675090.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNT1",
          "gene_hgnc_id": 18865,
          "hgvs_c": "c.3750C>G",
          "hgvs_p": "p.Asp1250Glu",
          "transcript": "XM_011518878.4",
          "protein_id": "XP_011517180.1",
          "transcript_support_level": null,
          "aa_start": 1250,
          "aa_end": null,
          "aa_length": 1283,
          "cds_start": 3750,
          "cds_end": null,
          "cds_length": 3852,
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      "gnomad_exomes_af": 6.88696e-7,
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      "gnomad_exomes_homalt": 0,
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      "computational_score_selected": 0.5113632082939148,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Benign",
      "alphamissense_score": 0.9256,
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      "bayesdelnoaf_score": -0.28,
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      "phylop100way_score": 1.93,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
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      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
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            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_020822.3",
          "gene_symbol": "KCNT1",
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          "effects": [
            "missense_variant"
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        {
          "score": 2,
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          ],
          "verdict": "Uncertain_significance",
          "transcript": "XR_007061851.1",
          "gene_symbol": "LOC107987140",
          "hgnc_id": null,
          "effects": [
            "upstream_gene_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.-18G>C",
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        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}