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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 9-19376299-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=9&pos=19376299&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "9",
      "pos": 19376299,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_001010.3",
      "consequences": [
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.744G>C",
          "hgvs_p": "p.Gln248His",
          "transcript": "NM_001010.3",
          "protein_id": "NP_001001.2",
          "transcript_support_level": null,
          "aa_start": 248,
          "aa_end": null,
          "aa_length": 249,
          "cds_start": 744,
          "cds_end": null,
          "cds_length": 750,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000380394.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001010.3"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.744G>C",
          "hgvs_p": "p.Gln248His",
          "transcript": "ENST00000380394.9",
          "protein_id": "ENSP00000369757.4",
          "transcript_support_level": 1,
          "aa_start": 248,
          "aa_end": null,
          "aa_length": 249,
          "cds_start": 744,
          "cds_end": null,
          "cds_length": 750,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001010.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000380394.9"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.651G>C",
          "hgvs_p": "p.Gln217His",
          "transcript": "ENST00000380384.5",
          "protein_id": "ENSP00000369745.1",
          "transcript_support_level": 1,
          "aa_start": 217,
          "aa_end": null,
          "aa_length": 218,
          "cds_start": 651,
          "cds_end": null,
          "cds_length": 657,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000380384.5"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.768G>C",
          "hgvs_p": "p.Gln256His",
          "transcript": "ENST00000889073.1",
          "protein_id": "ENSP00000559132.1",
          "transcript_support_level": null,
          "aa_start": 256,
          "aa_end": null,
          "aa_length": 257,
          "cds_start": 768,
          "cds_end": null,
          "cds_length": 774,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000889073.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.765G>C",
          "hgvs_p": "p.Gln255His",
          "transcript": "ENST00000919423.1",
          "protein_id": "ENSP00000589482.1",
          "transcript_support_level": null,
          "aa_start": 255,
          "aa_end": null,
          "aa_length": 256,
          "cds_start": 765,
          "cds_end": null,
          "cds_length": 771,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919423.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.762G>C",
          "hgvs_p": "p.Gln254His",
          "transcript": "ENST00000948755.1",
          "protein_id": "ENSP00000618814.1",
          "transcript_support_level": null,
          "aa_start": 254,
          "aa_end": null,
          "aa_length": 255,
          "cds_start": 762,
          "cds_end": null,
          "cds_length": 768,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000948755.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.747G>C",
          "hgvs_p": "p.Gln249His",
          "transcript": "ENST00000948752.1",
          "protein_id": "ENSP00000618811.1",
          "transcript_support_level": null,
          "aa_start": 249,
          "aa_end": null,
          "aa_length": 250,
          "cds_start": 747,
          "cds_end": null,
          "cds_length": 753,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000948752.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.741G>C",
          "hgvs_p": "p.Gln247His",
          "transcript": "ENST00000889074.1",
          "protein_id": "ENSP00000559133.1",
          "transcript_support_level": null,
          "aa_start": 247,
          "aa_end": null,
          "aa_length": 248,
          "cds_start": 741,
          "cds_end": null,
          "cds_length": 747,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000889074.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.735G>C",
          "hgvs_p": "p.Gln245His",
          "transcript": "ENST00000948753.1",
          "protein_id": "ENSP00000618812.1",
          "transcript_support_level": null,
          "aa_start": 245,
          "aa_end": null,
          "aa_length": 246,
          "cds_start": 735,
          "cds_end": null,
          "cds_length": 741,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000948753.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.732G>C",
          "hgvs_p": "p.Gln244His",
          "transcript": "ENST00000948751.1",
          "protein_id": "ENSP00000618810.1",
          "transcript_support_level": null,
          "aa_start": 244,
          "aa_end": null,
          "aa_length": 245,
          "cds_start": 732,
          "cds_end": null,
          "cds_length": 738,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000948751.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.723G>C",
          "hgvs_p": "p.Gln241His",
          "transcript": "ENST00000919422.1",
          "protein_id": "ENSP00000589481.1",
          "transcript_support_level": null,
          "aa_start": 241,
          "aa_end": null,
          "aa_length": 242,
          "cds_start": 723,
          "cds_end": null,
          "cds_length": 729,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919422.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.690G>C",
          "hgvs_p": "p.Gln230His",
          "transcript": "ENST00000889075.1",
          "protein_id": "ENSP00000559134.1",
          "transcript_support_level": null,
          "aa_start": 230,
          "aa_end": null,
          "aa_length": 231,
          "cds_start": 690,
          "cds_end": null,
          "cds_length": 696,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000889075.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.687G>C",
          "hgvs_p": "p.Gln229His",
          "transcript": "ENST00000889072.1",
          "protein_id": "ENSP00000559131.1",
          "transcript_support_level": null,
          "aa_start": 229,
          "aa_end": null,
          "aa_length": 230,
          "cds_start": 687,
          "cds_end": null,
          "cds_length": 693,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000889072.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.681G>C",
          "hgvs_p": "p.Gln227His",
          "transcript": "ENST00000919427.1",
          "protein_id": "ENSP00000589486.1",
          "transcript_support_level": null,
          "aa_start": 227,
          "aa_end": null,
          "aa_length": 228,
          "cds_start": 681,
          "cds_end": null,
          "cds_length": 687,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000919427.1"
        },
        {
          "aa_ref": "Q",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
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          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.657G>C",
          "hgvs_p": "p.Gln219His",
          "transcript": "ENST00000948750.1",
          "protein_id": "ENSP00000618809.1",
          "transcript_support_level": null,
          "aa_start": 219,
          "aa_end": null,
          "aa_length": 220,
          "cds_start": 657,
          "cds_end": null,
          "cds_length": 663,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000948750.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.654G>C",
          "hgvs_p": "p.Gln218His",
          "transcript": "ENST00000919421.1",
          "protein_id": "ENSP00000589480.1",
          "transcript_support_level": null,
          "aa_start": 218,
          "aa_end": null,
          "aa_length": 219,
          "cds_start": 654,
          "cds_end": null,
          "cds_length": 660,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919421.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.651G>C",
          "hgvs_p": "p.Gln217His",
          "transcript": "ENST00000315377.4",
          "protein_id": "ENSP00000369743.1",
          "transcript_support_level": 3,
          "aa_start": 217,
          "aa_end": null,
          "aa_length": 218,
          "cds_start": 651,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "Q",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.642G>C",
          "hgvs_p": "p.Gln214His",
          "transcript": "ENST00000919425.1",
          "protein_id": "ENSP00000589484.1",
          "transcript_support_level": null,
          "aa_start": 214,
          "aa_end": null,
          "aa_length": 215,
          "cds_start": 642,
          "cds_end": null,
          "cds_length": 648,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919425.1"
        },
        {
          "aa_ref": "Q",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.627G>C",
          "hgvs_p": "p.Gln209His",
          "transcript": "ENST00000919424.1",
          "protein_id": "ENSP00000589483.1",
          "transcript_support_level": null,
          "aa_start": 209,
          "aa_end": null,
          "aa_length": 210,
          "cds_start": 627,
          "cds_end": null,
          "cds_length": 633,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919424.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.612G>C",
          "hgvs_p": "p.Gln204His",
          "transcript": "ENST00000889076.1",
          "protein_id": "ENSP00000559135.1",
          "transcript_support_level": null,
          "aa_start": 204,
          "aa_end": null,
          "aa_length": 205,
          "cds_start": 612,
          "cds_end": null,
          "cds_length": 618,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
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        },
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          "gene_symbol": "RPS6",
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          "hgvs_p": "p.Gln175His",
          "transcript": "ENST00000919419.1",
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          "cds_start": 525,
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          "feature": "ENST00000919419.1"
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        {
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          "cds_start": 324,
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        {
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          "transcript": "ENST00000948754.1",
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          "biotype": "protein_coding",
          "feature": "ENST00000948754.1"
        },
        {
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          "protein_coding": true,
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          ],
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          "gene_symbol": "RPS6",
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          "hgvs_c": "c.192G>C",
          "hgvs_p": "p.Gln64His",
          "transcript": "ENST00000919428.1",
          "protein_id": "ENSP00000589487.1",
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          "cds_start": 192,
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          "cds_length": 198,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": null,
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          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
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          "exon_count": 2,
          "intron_rank": null,
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          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "n.436G>C",
          "hgvs_p": null,
          "transcript": "ENST00000498815.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
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          "aa_length": null,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000498815.1"
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      ],
      "gene_symbol": "RPS6",
      "gene_hgnc_id": 10429,
      "dbsnp": "rs757388872",
      "frequency_reference_population": 0.000030425901,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 49,
      "gnomad_exomes_af": 0.0000308578,
      "gnomad_genomes_af": 0.0000262864,
      "gnomad_exomes_ac": 45,
      "gnomad_genomes_ac": 4,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.40261685848236084,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.173,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.3315,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.23,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.968,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -5,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4,BS2",
      "acmg_by_gene": [
        {
          "score": -5,
          "benign_score": 5,
          "pathogenic_score": 0,
          "criteria": [
            "BP4",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001010.3",
          "gene_symbol": "RPS6",
          "hgnc_id": 10429,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.744G>C",
          "hgvs_p": "p.Gln248His"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}