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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 9-19376387-T-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=9&pos=19376387&ref=T&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "9",
      "pos": 19376387,
      "ref": "T",
      "alt": "G",
      "effect": "missense_variant,splice_region_variant",
      "transcript": "NM_001010.3",
      "consequences": [
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.656A>C",
          "hgvs_p": "p.Glu219Ala",
          "transcript": "NM_001010.3",
          "protein_id": "NP_001001.2",
          "transcript_support_level": null,
          "aa_start": 219,
          "aa_end": null,
          "aa_length": 249,
          "cds_start": 656,
          "cds_end": null,
          "cds_length": 750,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000380394.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001010.3"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.656A>C",
          "hgvs_p": "p.Glu219Ala",
          "transcript": "ENST00000380394.9",
          "protein_id": "ENSP00000369757.4",
          "transcript_support_level": 1,
          "aa_start": 219,
          "aa_end": null,
          "aa_length": 249,
          "cds_start": 656,
          "cds_end": null,
          "cds_length": 750,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001010.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000380394.9"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.563A>C",
          "hgvs_p": "p.Glu188Ala",
          "transcript": "ENST00000380384.5",
          "protein_id": "ENSP00000369745.1",
          "transcript_support_level": 1,
          "aa_start": 188,
          "aa_end": null,
          "aa_length": 218,
          "cds_start": 563,
          "cds_end": null,
          "cds_length": 657,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000380384.5"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.680A>C",
          "hgvs_p": "p.Glu227Ala",
          "transcript": "ENST00000889073.1",
          "protein_id": "ENSP00000559132.1",
          "transcript_support_level": null,
          "aa_start": 227,
          "aa_end": null,
          "aa_length": 257,
          "cds_start": 680,
          "cds_end": null,
          "cds_length": 774,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000889073.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.659A>C",
          "hgvs_p": "p.Glu220Ala",
          "transcript": "ENST00000948752.1",
          "protein_id": "ENSP00000618811.1",
          "transcript_support_level": null,
          "aa_start": 220,
          "aa_end": null,
          "aa_length": 250,
          "cds_start": 659,
          "cds_end": null,
          "cds_length": 753,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000948752.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.653A>C",
          "hgvs_p": "p.Glu218Ala",
          "transcript": "ENST00000889074.1",
          "protein_id": "ENSP00000559133.1",
          "transcript_support_level": null,
          "aa_start": 218,
          "aa_end": null,
          "aa_length": 248,
          "cds_start": 653,
          "cds_end": null,
          "cds_length": 747,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000889074.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.635A>C",
          "hgvs_p": "p.Glu212Ala",
          "transcript": "ENST00000919422.1",
          "protein_id": "ENSP00000589481.1",
          "transcript_support_level": null,
          "aa_start": 212,
          "aa_end": null,
          "aa_length": 242,
          "cds_start": 635,
          "cds_end": null,
          "cds_length": 729,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919422.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.602A>C",
          "hgvs_p": "p.Glu201Ala",
          "transcript": "ENST00000889075.1",
          "protein_id": "ENSP00000559134.1",
          "transcript_support_level": null,
          "aa_start": 201,
          "aa_end": null,
          "aa_length": 231,
          "cds_start": 602,
          "cds_end": null,
          "cds_length": 696,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000889075.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.599A>C",
          "hgvs_p": "p.Glu200Ala",
          "transcript": "ENST00000889072.1",
          "protein_id": "ENSP00000559131.1",
          "transcript_support_level": null,
          "aa_start": 200,
          "aa_end": null,
          "aa_length": 230,
          "cds_start": 599,
          "cds_end": null,
          "cds_length": 693,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000889072.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.593A>C",
          "hgvs_p": "p.Glu198Ala",
          "transcript": "ENST00000919427.1",
          "protein_id": "ENSP00000589486.1",
          "transcript_support_level": null,
          "aa_start": 198,
          "aa_end": null,
          "aa_length": 228,
          "cds_start": 593,
          "cds_end": null,
          "cds_length": 687,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919427.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.569A>C",
          "hgvs_p": "p.Glu190Ala",
          "transcript": "ENST00000948750.1",
          "protein_id": "ENSP00000618809.1",
          "transcript_support_level": null,
          "aa_start": 190,
          "aa_end": null,
          "aa_length": 220,
          "cds_start": 569,
          "cds_end": null,
          "cds_length": 663,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000948750.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.566A>C",
          "hgvs_p": "p.Glu189Ala",
          "transcript": "ENST00000919421.1",
          "protein_id": "ENSP00000589480.1",
          "transcript_support_level": null,
          "aa_start": 189,
          "aa_end": null,
          "aa_length": 219,
          "cds_start": 566,
          "cds_end": null,
          "cds_length": 660,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919421.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.563A>C",
          "hgvs_p": "p.Glu188Ala",
          "transcript": "ENST00000315377.4",
          "protein_id": "ENSP00000369743.1",
          "transcript_support_level": 3,
          "aa_start": 188,
          "aa_end": null,
          "aa_length": 218,
          "cds_start": 563,
          "cds_end": null,
          "cds_length": 657,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000315377.4"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.554A>C",
          "hgvs_p": "p.Glu185Ala",
          "transcript": "ENST00000919425.1",
          "protein_id": "ENSP00000589484.1",
          "transcript_support_level": null,
          "aa_start": 185,
          "aa_end": null,
          "aa_length": 215,
          "cds_start": 554,
          "cds_end": null,
          "cds_length": 648,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919425.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.539A>C",
          "hgvs_p": "p.Glu180Ala",
          "transcript": "ENST00000919424.1",
          "protein_id": "ENSP00000589483.1",
          "transcript_support_level": null,
          "aa_start": 180,
          "aa_end": null,
          "aa_length": 210,
          "cds_start": 539,
          "cds_end": null,
          "cds_length": 633,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919424.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.524A>C",
          "hgvs_p": "p.Glu175Ala",
          "transcript": "ENST00000889076.1",
          "protein_id": "ENSP00000559135.1",
          "transcript_support_level": null,
          "aa_start": 175,
          "aa_end": null,
          "aa_length": 205,
          "cds_start": 524,
          "cds_end": null,
          "cds_length": 618,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000889076.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.521A>C",
          "hgvs_p": "p.Glu174Ala",
          "transcript": "ENST00000919420.1",
          "protein_id": "ENSP00000589479.1",
          "transcript_support_level": null,
          "aa_start": 174,
          "aa_end": null,
          "aa_length": 204,
          "cds_start": 521,
          "cds_end": null,
          "cds_length": 615,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919420.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.509A>C",
          "hgvs_p": "p.Glu170Ala",
          "transcript": "ENST00000889077.1",
          "protein_id": "ENSP00000559136.1",
          "transcript_support_level": null,
          "aa_start": 170,
          "aa_end": null,
          "aa_length": 200,
          "cds_start": 509,
          "cds_end": null,
          "cds_length": 603,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000889077.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.437A>C",
          "hgvs_p": "p.Glu146Ala",
          "transcript": "ENST00000919419.1",
          "protein_id": "ENSP00000589478.1",
          "transcript_support_level": null,
          "aa_start": 146,
          "aa_end": null,
          "aa_length": 176,
          "cds_start": 437,
          "cds_end": null,
          "cds_length": 531,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919419.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6",
          "gene_hgnc_id": 10429,
          "hgvs_c": "c.236A>C",
          "hgvs_p": "p.Glu79Ala",
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          "biotype": "pseudogene",
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      "gene_symbol": "RPS6",
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      "dbsnp": "rs3209388",
      "frequency_reference_population": 6.841087e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84109e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.6413884162902832,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.843999981880188,
      "splice_prediction_selected": "Pathogenic",
      "splice_source_selected": "dbscSNV1_RF",
      "revel_score": 0.398,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.8163,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.01,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 7.955,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": 0.736349679116246,
      "dbscsnv_ada_prediction": "Benign",
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001010.3",
          "gene_symbol": "RPS6",
          "hgnc_id": 10429,
          "effects": [
            "missense_variant",
            "splice_region_variant"
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          "inheritance_mode": "AD",
          "hgvs_c": "c.656A>C",
          "hgvs_p": "p.Glu219Ala"
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      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}