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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 9-34635727-TGCTGAAGACAGTGTCG-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=9&pos=34635727&ref=TGCTGAAGACAGTGTCG&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "9",
      "pos": 34635727,
      "ref": "TGCTGAAGACAGTGTCG",
      "alt": "T",
      "effect": "frameshift_variant",
      "transcript": "ENST00000277010.9",
      "consequences": [
        {
          "aa_ref": "ADTVFS",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "c.561_576delCGACACTGTCTTCAGC",
          "hgvs_p": "p.Asp188fs",
          "transcript": "NM_005866.4",
          "protein_id": "NP_005857.1",
          "transcript_support_level": null,
          "aa_start": 187,
          "aa_end": null,
          "aa_length": 223,
          "cds_start": 561,
          "cds_end": null,
          "cds_length": 672,
          "cdna_start": 666,
          "cdna_end": null,
          "cdna_length": 1672,
          "mane_select": "ENST00000277010.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "ADTVFS",
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "c.561_576delCGACACTGTCTTCAGC",
          "hgvs_p": "p.Asp188fs",
          "transcript": "ENST00000277010.9",
          "protein_id": "ENSP00000277010.4",
          "transcript_support_level": 1,
          "aa_start": 187,
          "aa_end": null,
          "aa_length": 223,
          "cds_start": 561,
          "cds_end": null,
          "cds_length": 672,
          "cdna_start": 666,
          "cdna_end": null,
          "cdna_length": 1672,
          "mane_select": "NM_005866.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "ADTVFS",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "c.468_483delCGACACTGTCTTCAGC",
          "hgvs_p": "p.Asp157fs",
          "transcript": "ENST00000477726.1",
          "protein_id": "ENSP00000420022.1",
          "transcript_support_level": 1,
          "aa_start": 156,
          "aa_end": null,
          "aa_length": 192,
          "cds_start": 468,
          "cds_end": null,
          "cds_length": 579,
          "cdna_start": 595,
          "cdna_end": null,
          "cdna_length": 840,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "n.*193_*208delCGACACTGTCTTCAGC",
          "hgvs_p": null,
          "transcript": "ENST00000353468.4",
          "protein_id": "ENSP00000434453.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1582,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "n.693_708delCGACACTGTCTTCAGC",
          "hgvs_p": null,
          "transcript": "ENST00000461426.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 939,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "n.*193_*208delCGACACTGTCTTCAGC",
          "hgvs_p": null,
          "transcript": "ENST00000353468.4",
          "protein_id": "ENSP00000434453.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1582,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "ADTVFS",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "c.501_516delCGACACTGTCTTCAGC",
          "hgvs_p": "p.Asp168fs",
          "transcript": "NM_001282207.2",
          "protein_id": "NP_001269136.1",
          "transcript_support_level": null,
          "aa_start": 167,
          "aa_end": null,
          "aa_length": 203,
          "cds_start": 501,
          "cds_end": null,
          "cds_length": 612,
          "cdna_start": 606,
          "cdna_end": null,
          "cdna_length": 1612,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "ADTVFS",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "c.501_516delCGACACTGTCTTCAGC",
          "hgvs_p": "p.Asp168fs",
          "transcript": "ENST00000679597.1",
          "protein_id": "ENSP00000505634.1",
          "transcript_support_level": null,
          "aa_start": 167,
          "aa_end": null,
          "aa_length": 203,
          "cds_start": 501,
          "cds_end": null,
          "cds_length": 612,
          "cdna_start": 606,
          "cdna_end": null,
          "cdna_length": 1612,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "ADTVFS",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "c.468_483delCGACACTGTCTTCAGC",
          "hgvs_p": "p.Asp157fs",
          "transcript": "NM_147157.3",
          "protein_id": "NP_671513.1",
          "transcript_support_level": null,
          "aa_start": 156,
          "aa_end": null,
          "aa_length": 192,
          "cds_start": 468,
          "cds_end": null,
          "cds_length": 579,
          "cdna_start": 573,
          "cdna_end": null,
          "cdna_length": 1579,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "ADTVFS",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "c.294_309delCGACACTGTCTTCAGC",
          "hgvs_p": "p.Asp99fs",
          "transcript": "ENST00000378892.5",
          "protein_id": "ENSP00000368170.1",
          "transcript_support_level": 2,
          "aa_start": 98,
          "aa_end": null,
          "aa_length": 134,
          "cds_start": 294,
          "cds_end": null,
          "cds_length": 405,
          "cdna_start": 737,
          "cdna_end": null,
          "cdna_length": 1743,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "ADTVFS",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "c.261_276delCGACACTGTCTTCAGC",
          "hgvs_p": "p.Asp88fs",
          "transcript": "NM_001282206.2",
          "protein_id": "NP_001269135.1",
          "transcript_support_level": null,
          "aa_start": 87,
          "aa_end": null,
          "aa_length": 123,
          "cds_start": 261,
          "cds_end": null,
          "cds_length": 372,
          "cdna_start": 619,
          "cdna_end": null,
          "cdna_length": 1625,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "n.838_853delCGACACTGTCTTCAGC",
          "hgvs_p": null,
          "transcript": "ENST00000497006.2",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
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          "cdna_start": null,
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          "cdna_length": 1842,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
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          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "n.*731_*746delCGACACTGTCTTCAGC",
          "hgvs_p": null,
          "transcript": "ENST00000680104.1",
          "protein_id": "ENSP00000505949.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "cds_start": -4,
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          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "n.1948_1963delCGACACTGTCTTCAGC",
          "hgvs_p": null,
          "transcript": "ENST00000681409.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "consequences": [
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          ],
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          "exon_count": 4,
          "intron_rank": null,
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          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "n.538_553delCGACACTGTCTTCAGC",
          "hgvs_p": null,
          "transcript": "NR_104108.2",
          "protein_id": null,
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "c.*104_*119delCGACACTGTCTTCAGC",
          "hgvs_p": null,
          "transcript": "NM_001282208.2",
          "protein_id": "NP_001269137.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": 159,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 480,
          "cdna_start": null,
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          "cdna_length": 1695,
          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "c.*88_*103delCGACACTGTCTTCAGC",
          "hgvs_p": null,
          "transcript": "NM_001282209.2",
          "protein_id": "NP_001269138.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 110,
          "cds_start": -4,
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          "cds_length": 333,
          "cdna_start": null,
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          "cdna_length": 1532,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
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          "canonical": false,
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          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "c.*88_*103delCGACACTGTCTTCAGC",
          "hgvs_p": null,
          "transcript": "ENST00000680730.1",
          "protein_id": "ENSP00000505588.1",
          "transcript_support_level": null,
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          "cds_start": -4,
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          "cdna_start": null,
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          "feature": null
        },
        {
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          "consequences": [
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          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "n.*731_*746delCGACACTGTCTTCAGC",
          "hgvs_p": null,
          "transcript": "ENST00000680104.1",
          "protein_id": "ENSP00000505949.1",
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          "biotype": null,
          "feature": null
        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "c.446-103_446-88delCGACACTGTCTTCAGC",
          "hgvs_p": null,
          "transcript": "NM_001282205.2",
          "protein_id": "NP_001269134.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 155,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 468,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1454,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "c.446-103_446-88delCGACACTGTCTTCAGC",
          "hgvs_p": null,
          "transcript": "ENST00000680244.1",
          "protein_id": "ENSP00000505305.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 155,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 468,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 864,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SIGMAR1",
          "gene_hgnc_id": 8157,
          "hgvs_c": "c.*104_*119delCGACACTGTCTTCAGC",
          "hgvs_p": null,
          "transcript": "ENST00000680277.1",
          "protein_id": "ENSP00000505742.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 159,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 480,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 630,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "SIGMAR1",
      "gene_hgnc_id": 8157,
      "dbsnp": "rs1554707680",
      "frequency_reference_population": 0.0000018586186,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 3,
      "gnomad_exomes_af": 0.00000136809,
      "gnomad_genomes_af": 0.00000656987,
      "gnomad_exomes_ac": 2,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": null,
      "computational_prediction_selected": null,
      "computational_source_selected": null,
      "splice_score_selected": null,
      "splice_prediction_selected": null,
      "splice_source_selected": null,
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": null,
      "bayesdelnoaf_prediction": null,
      "phylop100way_score": 8.68,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": null,
      "spliceai_max_prediction": null,
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 12,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1_Strong,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 12,
          "benign_score": 0,
          "pathogenic_score": 12,
          "criteria": [
            "PVS1_Strong",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000277010.9",
          "gene_symbol": "SIGMAR1",
          "hgnc_id": 8157,
          "effects": [
            "frameshift_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.561_576delCGACACTGTCTTCAGC",
          "hgvs_p": "p.Asp188fs"
        }
      ],
      "clinvar_disease": "Amyotrophic lateral sclerosis type 16,Autosomal recessive distal spinal muscular atrophy 2",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LP:2",
      "phenotype_combined": "Amyotrophic lateral sclerosis type 16|Autosomal recessive distal spinal muscular atrophy 2;Amyotrophic lateral sclerosis type 16",
      "pathogenicity_classification_combined": "Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}