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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 9-83000462-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=9&pos=83000462&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "9",
      "pos": 83000462,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_152573.4",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASEF",
          "gene_hgnc_id": 26464,
          "hgvs_c": "c.1546A>G",
          "hgvs_p": "p.Arg516Gly",
          "transcript": "NM_152573.4",
          "protein_id": "NP_689786.2",
          "transcript_support_level": null,
          "aa_start": 516,
          "aa_end": null,
          "aa_length": 740,
          "cds_start": 1546,
          "cds_end": null,
          "cds_length": 2223,
          "cdna_start": 1821,
          "cdna_end": null,
          "cdna_length": 5585,
          "mane_select": "ENST00000376447.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_152573.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "G",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASEF",
          "gene_hgnc_id": 26464,
          "hgvs_c": "c.1546A>G",
          "hgvs_p": "p.Arg516Gly",
          "transcript": "ENST00000376447.4",
          "protein_id": "ENSP00000365630.3",
          "transcript_support_level": 1,
          "aa_start": 516,
          "aa_end": null,
          "aa_length": 740,
          "cds_start": 1546,
          "cds_end": null,
          "cds_length": 2223,
          "cdna_start": 1821,
          "cdna_end": null,
          "cdna_length": 5585,
          "mane_select": "NM_152573.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000376447.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASEF",
          "gene_hgnc_id": 26464,
          "hgvs_c": "c.1546A>G",
          "hgvs_p": "p.Arg516Gly",
          "transcript": "ENST00000928035.1",
          "protein_id": "ENSP00000598094.1",
          "transcript_support_level": null,
          "aa_start": 516,
          "aa_end": null,
          "aa_length": 735,
          "cds_start": 1546,
          "cds_end": null,
          "cds_length": 2208,
          "cdna_start": 1934,
          "cdna_end": null,
          "cdna_length": 5681,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928035.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASEF",
          "gene_hgnc_id": 26464,
          "hgvs_c": "c.1477A>G",
          "hgvs_p": "p.Arg493Gly",
          "transcript": "ENST00000873431.1",
          "protein_id": "ENSP00000543490.1",
          "transcript_support_level": null,
          "aa_start": 493,
          "aa_end": null,
          "aa_length": 717,
          "cds_start": 1477,
          "cds_end": null,
          "cds_length": 2154,
          "cdna_start": 1799,
          "cdna_end": null,
          "cdna_length": 5563,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000873431.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASEF",
          "gene_hgnc_id": 26464,
          "hgvs_c": "c.1468A>G",
          "hgvs_p": "p.Arg490Gly",
          "transcript": "ENST00000873434.1",
          "protein_id": "ENSP00000543493.1",
          "transcript_support_level": null,
          "aa_start": 490,
          "aa_end": null,
          "aa_length": 714,
          "cds_start": 1468,
          "cds_end": null,
          "cds_length": 2145,
          "cdna_start": 1489,
          "cdna_end": null,
          "cdna_length": 2457,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000873434.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASEF",
          "gene_hgnc_id": 26464,
          "hgvs_c": "c.1399A>G",
          "hgvs_p": "p.Arg467Gly",
          "transcript": "ENST00000873432.1",
          "protein_id": "ENSP00000543491.1",
          "transcript_support_level": null,
          "aa_start": 467,
          "aa_end": null,
          "aa_length": 691,
          "cds_start": 1399,
          "cds_end": null,
          "cds_length": 2076,
          "cdna_start": 1684,
          "cdna_end": null,
          "cdna_length": 5448,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000873432.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASEF",
          "gene_hgnc_id": 26464,
          "hgvs_c": "c.1330A>G",
          "hgvs_p": "p.Arg444Gly",
          "transcript": "ENST00000873433.1",
          "protein_id": "ENSP00000543492.1",
          "transcript_support_level": null,
          "aa_start": 444,
          "aa_end": null,
          "aa_length": 668,
          "cds_start": 1330,
          "cds_end": null,
          "cds_length": 2007,
          "cdna_start": 1605,
          "cdna_end": null,
          "cdna_length": 5369,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000873433.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASEF",
          "gene_hgnc_id": 26464,
          "hgvs_c": "c.1477A>G",
          "hgvs_p": "p.Arg493Gly",
          "transcript": "XM_005251730.5",
          "protein_id": "XP_005251787.1",
          "transcript_support_level": null,
          "aa_start": 493,
          "aa_end": null,
          "aa_length": 717,
          "cds_start": 1477,
          "cds_end": null,
          "cds_length": 2154,
          "cdna_start": 1752,
          "cdna_end": null,
          "cdna_length": 5516,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005251730.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASEF",
          "gene_hgnc_id": 26464,
          "hgvs_c": "c.1159A>G",
          "hgvs_p": "p.Arg387Gly",
          "transcript": "XM_005251731.5",
          "protein_id": "XP_005251788.1",
          "transcript_support_level": null,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 611,
          "cds_start": 1159,
          "cds_end": null,
          "cds_length": 1836,
          "cdna_start": 2293,
          "cdna_end": null,
          "cdna_length": 6057,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005251731.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASEF",
          "gene_hgnc_id": 26464,
          "hgvs_c": "c.1090A>G",
          "hgvs_p": "p.Arg364Gly",
          "transcript": "XM_047422826.1",
          "protein_id": "XP_047278782.1",
          "transcript_support_level": null,
          "aa_start": 364,
          "aa_end": null,
          "aa_length": 588,
          "cds_start": 1090,
          "cds_end": null,
          "cds_length": 1767,
          "cdna_start": 2224,
          "cdna_end": null,
          "cdna_length": 5988,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047422826.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASEF",
          "gene_hgnc_id": 26464,
          "hgvs_c": "c.997A>G",
          "hgvs_p": "p.Arg333Gly",
          "transcript": "XM_047422827.1",
          "protein_id": "XP_047278783.1",
          "transcript_support_level": null,
          "aa_start": 333,
          "aa_end": null,
          "aa_length": 557,
          "cds_start": 997,
          "cds_end": null,
          "cds_length": 1674,
          "cdna_start": 1304,
          "cdna_end": null,
          "cdna_length": 5068,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047422827.1"
        }
      ],
      "gene_symbol": "RASEF",
      "gene_hgnc_id": 26464,
      "dbsnp": "rs147216417",
      "frequency_reference_population": 0.0007645137,
      "hom_count_reference_population": 1,
      "allele_count_reference_population": 1234,
      "gnomad_exomes_af": 0.000774367,
      "gnomad_genomes_af": 0.000669916,
      "gnomad_exomes_ac": 1132,
      "gnomad_genomes_ac": 102,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 1,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.0632442831993103,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.267,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.2473,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.09,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.146,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_152573.4",
          "gene_symbol": "RASEF",
          "hgnc_id": 26464,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1546A>G",
          "hgvs_p": "p.Arg516Gly"
        }
      ],
      "clinvar_disease": "RASEF-related disorder",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "RASEF-related disorder",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}
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