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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 9-87727613-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=9&pos=87727613&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "9",
      "pos": 87727613,
      "ref": "A",
      "alt": "G",
      "effect": "5_prime_UTR_premature_start_codon_gain_variant",
      "transcript": "NM_001257973.2",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSL",
          "gene_hgnc_id": 2537,
          "hgvs_c": "c.10A>G",
          "hgvs_p": "p.Thr4Ala",
          "transcript": "NM_001912.5",
          "protein_id": "NP_001903.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 333,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1002,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000343150.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001912.5"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSL",
          "gene_hgnc_id": 2537,
          "hgvs_c": "c.10A>G",
          "hgvs_p": "p.Thr4Ala",
          "transcript": "ENST00000343150.10",
          "protein_id": "ENSP00000345344.5",
          "transcript_support_level": 1,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 333,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1002,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001912.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000343150.10"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSL",
          "gene_hgnc_id": 2537,
          "hgvs_c": "c.10A>G",
          "hgvs_p": "p.Thr4Ala",
          "transcript": "ENST00000340342.11",
          "protein_id": "ENSP00000365061.5",
          "transcript_support_level": 1,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 333,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1002,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000340342.11"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSL",
          "gene_hgnc_id": 2537,
          "hgvs_c": "n.119A>G",
          "hgvs_p": null,
          "transcript": "ENST00000495822.2",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000495822.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_premature_start_codon_gain_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSL",
          "gene_hgnc_id": 2537,
          "hgvs_c": "c.-107A>G",
          "hgvs_p": null,
          "transcript": "NM_001257973.2",
          "protein_id": "NP_001244902.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 151,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 456,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001257973.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSL",
          "gene_hgnc_id": 2537,
          "hgvs_c": "c.10A>G",
          "hgvs_p": "p.Thr4Ala",
          "transcript": "ENST00000678596.1",
          "protein_id": "ENSP00000503146.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 341,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1026,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000678596.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSL",
          "gene_hgnc_id": 2537,
          "hgvs_c": "c.10A>G",
          "hgvs_p": "p.Thr4Ala",
          "transcript": "ENST00000678649.1",
          "protein_id": "ENSP00000504772.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 341,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1026,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000678649.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSL",
          "gene_hgnc_id": 2537,
          "hgvs_c": "c.10A>G",
          "hgvs_p": "p.Thr4Ala",
          "transcript": "NM_001257971.2",
          "protein_id": "NP_001244900.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 333,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1002,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001257971.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSL",
          "gene_hgnc_id": 2537,
          "hgvs_c": "c.10A>G",
          "hgvs_p": "p.Thr4Ala",
          "transcript": "NM_001257972.2",
          "protein_id": "NP_001244901.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 333,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1002,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001257972.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSL",
          "gene_hgnc_id": 2537,
          "hgvs_c": "c.10A>G",
          "hgvs_p": "p.Thr4Ala",
          "transcript": "NM_001382757.1",
          "protein_id": "NP_001369686.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 333,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1002,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001382757.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSL",
          "gene_hgnc_id": 2537,
          "hgvs_c": "c.10A>G",
          "hgvs_p": "p.Thr4Ala",
          "transcript": "NM_145918.3",
          "protein_id": "NP_666023.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 333,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1002,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_145918.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSL",
          "gene_hgnc_id": 2537,
          "hgvs_c": "c.10A>G",
          "hgvs_p": "p.Thr4Ala",
          "transcript": "ENST00000676480.1",
          "protein_id": "ENSP00000504279.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 333,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1002,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000676480.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSL",
          "gene_hgnc_id": 2537,
          "hgvs_c": "c.10A>G",
          "hgvs_p": "p.Thr4Ala",
          "transcript": "ENST00000676531.1",
          "protein_id": "ENSP00000503439.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 333,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1002,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000676531.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSL",
          "gene_hgnc_id": 2537,
          "hgvs_c": "c.10A>G",
          "hgvs_p": "p.Thr4Ala",
          "transcript": "ENST00000676881.1",
          "protein_id": "ENSP00000502901.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 333,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1002,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000676881.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSL",
          "gene_hgnc_id": 2537,
          "hgvs_c": "c.10A>G",
          "hgvs_p": "p.Thr4Ala",
          "transcript": "ENST00000676946.1",
          "protein_id": "ENSP00000503470.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 333,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1002,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000676946.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSL",
          "gene_hgnc_id": 2537,
          "hgvs_c": "c.10A>G",
          "hgvs_p": "p.Thr4Ala",
          "transcript": "ENST00000677262.1",
          "protein_id": "ENSP00000503851.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 333,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1002,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000677262.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSL",
          "gene_hgnc_id": 2537,
          "hgvs_c": "c.10A>G",
          "hgvs_p": "p.Thr4Ala",
          "transcript": "ENST00000677761.1",
          "protein_id": "ENSP00000503938.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 333,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1002,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000677761.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSL",
          "gene_hgnc_id": 2537,
          "hgvs_c": "c.10A>G",
          "hgvs_p": "p.Thr4Ala",
          "transcript": "ENST00000677821.1",
          "protein_id": "ENSP00000503298.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 333,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1002,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000677821.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSL",
          "gene_hgnc_id": 2537,
          "hgvs_c": "c.10A>G",
          "hgvs_p": "p.Thr4Ala",
          "transcript": "ENST00000678442.1",
          "protein_id": "ENSP00000503897.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 333,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1002,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000678442.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSL",
          "gene_hgnc_id": 2537,
          "hgvs_c": "c.10A>G",
          "hgvs_p": "p.Thr4Ala",
          "transcript": "ENST00000679149.1",
          "protein_id": "ENSP00000504313.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 333,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1002,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000679149.1"
        },
        {
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        {
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          "gene_symbol": "ENSG00000304058",
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          "biotype": "pseudogene",
          "feature": "ENST00000799159.1"
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      ],
      "gene_symbol": "CTSL",
      "gene_hgnc_id": 2537,
      "dbsnp": "rs982050868",
      "frequency_reference_population": 0.0000037175641,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 6,
      "gnomad_exomes_af": 0.00000273623,
      "gnomad_genomes_af": 0.0000131499,
      "gnomad_exomes_ac": 4,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.05692094564437866,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.018,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0595,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.64,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.015,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001257973.2",
          "gene_symbol": "CTSL",
          "hgnc_id": 2537,
          "effects": [
            "5_prime_UTR_premature_start_codon_gain_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.-107A>G",
          "hgvs_p": null
        },
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000799159.1",
          "gene_symbol": "ENSG00000304058",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.46-4593T>C",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}