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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: X-101356177-A-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=X&pos=101356177&ref=A&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "X",
      "pos": 101356177,
      "ref": "A",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001287344.2",
      "consequences": [
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BTK",
          "gene_hgnc_id": 1133,
          "hgvs_c": "c.1441T>A",
          "hgvs_p": "p.Cys481Ser",
          "transcript": "NM_000061.3",
          "protein_id": "NP_000052.1",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 659,
          "cds_start": 1441,
          "cds_end": null,
          "cds_length": 1980,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000308731.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000061.3"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BTK",
          "gene_hgnc_id": 1133,
          "hgvs_c": "c.1441T>A",
          "hgvs_p": "p.Cys481Ser",
          "transcript": "ENST00000308731.8",
          "protein_id": "ENSP00000308176.8",
          "transcript_support_level": 1,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 659,
          "cds_start": 1441,
          "cds_end": null,
          "cds_length": 1980,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000061.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000308731.8"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BTK",
          "gene_hgnc_id": 1133,
          "hgvs_c": "c.1543T>A",
          "hgvs_p": "p.Cys515Ser",
          "transcript": "ENST00000621635.4",
          "protein_id": "ENSP00000483570.1",
          "transcript_support_level": 1,
          "aa_start": 515,
          "aa_end": null,
          "aa_length": 693,
          "cds_start": 1543,
          "cds_end": null,
          "cds_length": 2082,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000621635.4"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BTK",
          "gene_hgnc_id": 1133,
          "hgvs_c": "c.1543T>A",
          "hgvs_p": "p.Cys515Ser",
          "transcript": "NM_001287344.2",
          "protein_id": "NP_001274273.1",
          "transcript_support_level": null,
          "aa_start": 515,
          "aa_end": null,
          "aa_length": 693,
          "cds_start": 1543,
          "cds_end": null,
          "cds_length": 2082,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001287344.2"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BTK",
          "gene_hgnc_id": 1133,
          "hgvs_c": "c.1522T>A",
          "hgvs_p": "p.Cys508Ser",
          "transcript": "ENST00000944957.1",
          "protein_id": "ENSP00000615016.1",
          "transcript_support_level": null,
          "aa_start": 508,
          "aa_end": null,
          "aa_length": 686,
          "cds_start": 1522,
          "cds_end": null,
          "cds_length": 2061,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000944957.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BTK",
          "gene_hgnc_id": 1133,
          "hgvs_c": "c.1441T>A",
          "hgvs_p": "p.Cys481Ser",
          "transcript": "ENST00000695614.1",
          "protein_id": "ENSP00000512053.1",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 659,
          "cds_start": 1441,
          "cds_end": null,
          "cds_length": 1980,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000695614.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BTK",
          "gene_hgnc_id": 1133,
          "hgvs_c": "c.1441T>A",
          "hgvs_p": "p.Cys481Ser",
          "transcript": "ENST00000695615.1",
          "protein_id": "ENSP00000512054.1",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 659,
          "cds_start": 1441,
          "cds_end": null,
          "cds_length": 1980,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000695615.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BTK",
          "gene_hgnc_id": 1133,
          "hgvs_c": "c.1441T>A",
          "hgvs_p": "p.Cys481Ser",
          "transcript": "ENST00000896540.1",
          "protein_id": "ENSP00000566599.1",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 659,
          "cds_start": 1441,
          "cds_end": null,
          "cds_length": 1980,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000896540.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BTK",
          "gene_hgnc_id": 1133,
          "hgvs_c": "c.1441T>A",
          "hgvs_p": "p.Cys481Ser",
          "transcript": "ENST00000944955.1",
          "protein_id": "ENSP00000615014.1",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 659,
          "cds_start": 1441,
          "cds_end": null,
          "cds_length": 1980,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000944955.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BTK",
          "gene_hgnc_id": 1133,
          "hgvs_c": "c.1438T>A",
          "hgvs_p": "p.Cys480Ser",
          "transcript": "ENST00000695617.1",
          "protein_id": "ENSP00000512056.1",
          "transcript_support_level": null,
          "aa_start": 480,
          "aa_end": null,
          "aa_length": 658,
          "cds_start": 1438,
          "cds_end": null,
          "cds_length": 1977,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000695617.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BTK",
          "gene_hgnc_id": 1133,
          "hgvs_c": "c.1435T>A",
          "hgvs_p": "p.Cys479Ser",
          "transcript": "ENST00000695623.1",
          "protein_id": "ENSP00000512063.1",
          "transcript_support_level": null,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 657,
          "cds_start": 1435,
          "cds_end": null,
          "cds_length": 1974,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000695623.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BTK",
          "gene_hgnc_id": 1133,
          "hgvs_c": "c.1441T>A",
          "hgvs_p": "p.Cys481Ser",
          "transcript": "ENST00000695625.1",
          "protein_id": "ENSP00000512064.1",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 648,
          "cds_start": 1441,
          "cds_end": null,
          "cds_length": 1947,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000695625.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BTK",
          "gene_hgnc_id": 1133,
          "hgvs_c": "c.1378T>A",
          "hgvs_p": "p.Cys460Ser",
          "transcript": "ENST00000695622.1",
          "protein_id": "ENSP00000512062.1",
          "transcript_support_level": null,
          "aa_start": 460,
          "aa_end": null,
          "aa_length": 638,
          "cds_start": 1378,
          "cds_end": null,
          "cds_length": 1917,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000695622.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BTK",
          "gene_hgnc_id": 1133,
          "hgvs_c": "c.1342T>A",
          "hgvs_p": "p.Cys448Ser",
          "transcript": "ENST00000914420.1",
          "protein_id": "ENSP00000584479.1",
          "transcript_support_level": null,
          "aa_start": 448,
          "aa_end": null,
          "aa_length": 626,
          "cds_start": 1342,
          "cds_end": null,
          "cds_length": 1881,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000914420.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BTK",
          "gene_hgnc_id": 1133,
          "hgvs_c": "c.1312T>A",
          "hgvs_p": "p.Cys438Ser",
          "transcript": "ENST00000944956.1",
          "protein_id": "ENSP00000615015.1",
          "transcript_support_level": null,
          "aa_start": 438,
          "aa_end": null,
          "aa_length": 616,
          "cds_start": 1312,
          "cds_end": null,
          "cds_length": 1851,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000944956.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BTK",
          "gene_hgnc_id": 1133,
          "hgvs_c": "c.1306T>A",
          "hgvs_p": "p.Cys436Ser",
          "transcript": "ENST00000944958.1",
          "protein_id": "ENSP00000615017.1",
          "transcript_support_level": null,
          "aa_start": 436,
          "aa_end": null,
          "aa_length": 614,
          "cds_start": 1306,
          "cds_end": null,
          "cds_length": 1845,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000944958.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": 13,
          "intron_rank_end": null,
          "gene_symbol": "BTK",
          "gene_hgnc_id": 1133,
          "hgvs_c": "c.1039-1483T>A",
          "hgvs_p": null,
          "transcript": "NM_001287345.2",
          "protein_id": "NP_001274274.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 483,
          "cds_start": null,
          "cds_end": null,
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          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001287345.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": 13,
          "intron_rank_end": null,
          "gene_symbol": "BTK",
          "gene_hgnc_id": 1133,
          "hgvs_c": "c.1039-1483T>A",
          "hgvs_p": null,
          "transcript": "ENST00000703407.1",
          "protein_id": "ENSP00000512057.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 483,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1452,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000703407.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "BTK",
          "gene_hgnc_id": 1133,
          "hgvs_c": "c.356+607T>A",
          "hgvs_p": null,
          "transcript": "ENST00000695630.1",
          "protein_id": "ENSP00000512069.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": null,
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          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000695630.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "BTK",
          "gene_hgnc_id": 1133,
          "hgvs_c": "c.190+1332T>A",
          "hgvs_p": null,
          "transcript": "ENST00000695629.1",
          "protein_id": "ENSP00000512068.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 139,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 420,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000695629.1"
        },
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      "dbsnp": "rs1057519826",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.869105339050293,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.565,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.6012,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.04,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 7.313,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
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      "mitotip_score": null,
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      "acmg_score": 8,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PS1_Moderate,PM2,PP2,PP3_Moderate,PP5",
      "acmg_by_gene": [
        {
          "score": 8,
          "benign_score": 0,
          "pathogenic_score": 8,
          "criteria": [
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            "PM2",
            "PP2",
            "PP3_Moderate",
            "PP5"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_001287344.2",
          "gene_symbol": "BTK",
          "hgnc_id": 1133,
          "effects": [
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          "inheritance_mode": "XL",
          "hgvs_c": "c.1543T>A",
          "hgvs_p": "p.Cys515Ser"
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      ],
      "clinvar_disease": "X-linked agammaglobulinemia with growth hormone deficiency,not provided",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "P:1 US:2",
      "phenotype_combined": "X-linked agammaglobulinemia with growth hormone deficiency|not provided",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
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  ],
  "message": null
}