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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: X-12717893-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=X&pos=12717893&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "X",
      "pos": 12717893,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "ENST00000675598.1",
      "consequences": [
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRMPD4",
          "gene_hgnc_id": 29007,
          "hgvs_c": "c.3067T>C",
          "hgvs_p": "p.Cys1023Arg",
          "transcript": "NM_001368397.1",
          "protein_id": "NP_001355326.1",
          "transcript_support_level": null,
          "aa_start": 1023,
          "aa_end": null,
          "aa_length": 1762,
          "cds_start": 3067,
          "cds_end": null,
          "cds_length": 5289,
          "cdna_start": 3566,
          "cdna_end": null,
          "cdna_length": 8453,
          "mane_select": "ENST00000675598.1",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRMPD4",
          "gene_hgnc_id": 29007,
          "hgvs_c": "c.3067T>C",
          "hgvs_p": "p.Cys1023Arg",
          "transcript": "ENST00000675598.1",
          "protein_id": "ENSP00000502607.1",
          "transcript_support_level": null,
          "aa_start": 1023,
          "aa_end": null,
          "aa_length": 1762,
          "cds_start": 3067,
          "cds_end": null,
          "cds_length": 5289,
          "cdna_start": 3566,
          "cdna_end": null,
          "cdna_length": 8453,
          "mane_select": "NM_001368397.1",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRMPD4",
          "gene_hgnc_id": 29007,
          "hgvs_c": "c.3067T>C",
          "hgvs_p": "p.Cys1023Arg",
          "transcript": "ENST00000380682.5",
          "protein_id": "ENSP00000370057.1",
          "transcript_support_level": 1,
          "aa_start": 1023,
          "aa_end": null,
          "aa_length": 1322,
          "cds_start": 3067,
          "cds_end": null,
          "cds_length": 3969,
          "cdna_start": 3573,
          "cdna_end": null,
          "cdna_length": 8465,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRMPD4",
          "gene_hgnc_id": 29007,
          "hgvs_c": "c.3178T>C",
          "hgvs_p": "p.Cys1060Arg",
          "transcript": "NM_001368395.3",
          "protein_id": "NP_001355324.1",
          "transcript_support_level": null,
          "aa_start": 1060,
          "aa_end": null,
          "aa_length": 1799,
          "cds_start": 3178,
          "cds_end": null,
          "cds_length": 5400,
          "cdna_start": 3558,
          "cdna_end": null,
          "cdna_length": 8445,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRMPD4",
          "gene_hgnc_id": 29007,
          "hgvs_c": "c.3121T>C",
          "hgvs_p": "p.Cys1041Arg",
          "transcript": "ENST00000656302.1",
          "protein_id": "ENSP00000499481.1",
          "transcript_support_level": null,
          "aa_start": 1041,
          "aa_end": null,
          "aa_length": 1780,
          "cds_start": 3121,
          "cds_end": null,
          "cds_length": 5343,
          "cdna_start": 3574,
          "cdna_end": null,
          "cdna_length": 6123,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRMPD4",
          "gene_hgnc_id": 29007,
          "hgvs_c": "c.3073T>C",
          "hgvs_p": "p.Cys1025Arg",
          "transcript": "NM_001368396.3",
          "protein_id": "NP_001355325.1",
          "transcript_support_level": null,
          "aa_start": 1025,
          "aa_end": null,
          "aa_length": 1764,
          "cds_start": 3073,
          "cds_end": null,
          "cds_length": 5295,
          "cdna_start": 3572,
          "cdna_end": null,
          "cdna_length": 8459,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRMPD4",
          "gene_hgnc_id": 29007,
          "hgvs_c": "c.3178T>C",
          "hgvs_p": "p.Cys1060Arg",
          "transcript": "NM_001368398.3",
          "protein_id": "NP_001355327.1",
          "transcript_support_level": null,
          "aa_start": 1060,
          "aa_end": null,
          "aa_length": 1359,
          "cds_start": 3178,
          "cds_end": null,
          "cds_length": 4080,
          "cdna_start": 3558,
          "cdna_end": null,
          "cdna_length": 8450,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRMPD4",
          "gene_hgnc_id": 29007,
          "hgvs_c": "c.3121T>C",
          "hgvs_p": "p.Cys1041Arg",
          "transcript": "ENST00000640291.2",
          "protein_id": "ENSP00000492353.2",
          "transcript_support_level": 5,
          "aa_start": 1041,
          "aa_end": null,
          "aa_length": 1340,
          "cds_start": 3121,
          "cds_end": null,
          "cds_length": 4023,
          "cdna_start": 3557,
          "cdna_end": null,
          "cdna_length": 6047,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRMPD4",
          "gene_hgnc_id": 29007,
          "hgvs_c": "c.3121T>C",
          "hgvs_p": "p.Cys1041Arg",
          "transcript": "ENST00000672869.2",
          "protein_id": "ENSP00000500566.2",
          "transcript_support_level": null,
          "aa_start": 1041,
          "aa_end": null,
          "aa_length": 1338,
          "cds_start": 3121,
          "cds_end": null,
          "cds_length": 4018,
          "cdna_start": 3282,
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          "cdna_length": 4179,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
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          "gene_symbol": "FRMPD4",
          "gene_hgnc_id": 29007,
          "hgvs_c": "c.3067T>C",
          "hgvs_p": "p.Cys1023Arg",
          "transcript": "NM_014728.3",
          "protein_id": "NP_055543.2",
          "transcript_support_level": null,
          "aa_start": 1023,
          "aa_end": null,
          "aa_length": 1322,
          "cds_start": 3067,
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          "cdna_start": 3573,
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        },
        {
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          "protein_coding": true,
          "strand": true,
          "consequences": [
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          "intron_rank": null,
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          "gene_symbol": "FRMPD4",
          "gene_hgnc_id": 29007,
          "hgvs_c": "c.3058T>C",
          "hgvs_p": "p.Cys1020Arg",
          "transcript": "NM_001368399.3",
          "protein_id": "NP_001355328.1",
          "transcript_support_level": null,
          "aa_start": 1020,
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          "cds_start": 3058,
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          "cdna_start": 3377,
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          "mane_select": null,
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        },
        {
          "aa_ref": "C",
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          "exon_rank": 16,
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          "exon_count": 17,
          "intron_rank": null,
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          "gene_symbol": "FRMPD4",
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          "hgvs_p": "p.Cys1020Arg",
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        {
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          "strand": true,
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          "intron_rank": null,
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          "gene_symbol": "FRMPD4",
          "gene_hgnc_id": 29007,
          "hgvs_c": "c.3043T>C",
          "hgvs_p": "p.Cys1015Arg",
          "transcript": "NM_001368401.1",
          "protein_id": "NP_001355330.1",
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        },
        {
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          ],
          "exon_rank": 17,
          "exon_rank_end": null,
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          "intron_rank": null,
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          "gene_symbol": "FRMPD4",
          "gene_hgnc_id": 29007,
          "hgvs_c": "c.3043T>C",
          "hgvs_p": "p.Cys1015Arg",
          "transcript": "NM_001368402.3",
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        },
        {
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          "hgvs_p": "p.Cys983Arg",
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        },
        {
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          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 17,
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          "exon_count": 18,
          "intron_rank": null,
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          "gene_symbol": "FRMPD4",
          "gene_hgnc_id": 29007,
          "hgvs_c": "c.2947T>C",
          "hgvs_p": "p.Cys983Arg",
          "transcript": "ENST00000616992.2",
          "protein_id": "ENSP00000482182.2",
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        {
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          "intron_rank": null,
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          "gene_symbol": "FRMPD4",
          "gene_hgnc_id": 29007,
          "hgvs_c": "c.3067T>C",
          "hgvs_p": "p.Cys1023Arg",
          "transcript": "XM_005274632.4",
          "protein_id": "XP_005274689.3",
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        {
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          "gene_symbol": "FRMPD4",
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          "transcript": "XM_017029983.2",
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        {
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        {
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          ],
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          "intron_rank": null,
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          "gene_symbol": "FRMPD4",
          "gene_hgnc_id": 29007,
          "hgvs_c": "c.3067T>C",
          "hgvs_p": "p.Cys1023Arg",
          "transcript": "XM_017029985.2",
          "protein_id": "XP_016885474.2",
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          "cdna_start": 3131,
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        },
        {
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          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRMPD4",
          "gene_hgnc_id": 29007,
          "hgvs_c": "c.2968T>C",
          "hgvs_p": "p.Cys990Arg",
          "transcript": "XM_047442680.1",
          "protein_id": "XP_047298636.1",
          "transcript_support_level": null,
          "aa_start": 990,
          "aa_end": null,
          "aa_length": 1289,
          "cds_start": 2968,
          "cds_end": null,
          "cds_length": 3870,
          "cdna_start": 3287,
          "cdna_end": null,
          "cdna_length": 8179,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRMPD4",
          "gene_hgnc_id": 29007,
          "hgvs_c": "n.3264T>C",
          "hgvs_p": null,
          "transcript": "ENST00000657982.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5862,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRMPD4",
          "gene_hgnc_id": 29007,
          "hgvs_c": "n.3720T>C",
          "hgvs_p": null,
          "transcript": "ENST00000673271.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 8605,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "FRMPD4",
      "gene_hgnc_id": 29007,
      "dbsnp": "rs143929343",
      "frequency_reference_population": 0.00016526727,
      "hom_count_reference_population": 65,
      "allele_count_reference_population": 200,
      "gnomad_exomes_af": 0.000176679,
      "gnomad_genomes_af": 0.0000535122,
      "gnomad_exomes_ac": 194,
      "gnomad_genomes_ac": 6,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.7220628261566162,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.301,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.4948,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.18,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 5.414,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -5,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP6,BS2",
      "acmg_by_gene": [
        {
          "score": -5,
          "benign_score": 5,
          "pathogenic_score": 0,
          "criteria": [
            "BP6",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000675598.1",
          "gene_symbol": "FRMPD4",
          "hgnc_id": 29007,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "XL",
          "hgvs_c": "c.3067T>C",
          "hgvs_p": "p.Cys1023Arg"
        }
      ],
      "clinvar_disease": "Inborn genetic diseases,not provided,not specified",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:1 LB:2",
      "phenotype_combined": "not specified|not provided|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}