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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: X-149498223-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=X&pos=149498223&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "X",
      "pos": 149498223,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "ENST00000340855.11",
      "consequences": [
        {
          "aa_ref": "D",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IDS",
          "gene_hgnc_id": 5389,
          "hgvs_c": "c.592G>A",
          "hgvs_p": "p.Asp198Asn",
          "transcript": "NM_000202.8",
          "protein_id": "NP_000193.1",
          "transcript_support_level": null,
          "aa_start": 198,
          "aa_end": null,
          "aa_length": 550,
          "cds_start": 592,
          "cds_end": null,
          "cds_length": 1653,
          "cdna_start": 761,
          "cdna_end": null,
          "cdna_length": 7580,
          "mane_select": "ENST00000340855.11",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "D",
          "aa_alt": "N",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IDS",
          "gene_hgnc_id": 5389,
          "hgvs_c": "c.592G>A",
          "hgvs_p": "p.Asp198Asn",
          "transcript": "ENST00000340855.11",
          "protein_id": "ENSP00000339801.6",
          "transcript_support_level": 1,
          "aa_start": 198,
          "aa_end": null,
          "aa_length": 550,
          "cds_start": 592,
          "cds_end": null,
          "cds_length": 1653,
          "cdna_start": 761,
          "cdna_end": null,
          "cdna_length": 7580,
          "mane_select": "NM_000202.8",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "D",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IDS",
          "gene_hgnc_id": 5389,
          "hgvs_c": "c.592G>A",
          "hgvs_p": "p.Asp198Asn",
          "transcript": "ENST00000370441.8",
          "protein_id": "ENSP00000359470.4",
          "transcript_support_level": 1,
          "aa_start": 198,
          "aa_end": null,
          "aa_length": 343,
          "cds_start": 592,
          "cds_end": null,
          "cds_length": 1032,
          "cdna_start": 802,
          "cdna_end": null,
          "cdna_length": 1399,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IDS",
          "gene_hgnc_id": 5389,
          "hgvs_c": "n.592G>A",
          "hgvs_p": null,
          "transcript": "ENST00000466323.5",
          "protein_id": "ENSP00000418264.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1529,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000241489",
          "gene_hgnc_id": null,
          "hgvs_c": "c.-42G>A",
          "hgvs_p": null,
          "transcript": "ENST00000651111.1",
          "protein_id": "ENSP00000498395.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 339,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 1020,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 9159,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "D",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IDS",
          "gene_hgnc_id": 5389,
          "hgvs_c": "c.322G>A",
          "hgvs_p": "p.Asp108Asn",
          "transcript": "NM_001166550.4",
          "protein_id": "NP_001160022.1",
          "transcript_support_level": null,
          "aa_start": 108,
          "aa_end": null,
          "aa_length": 460,
          "cds_start": 322,
          "cds_end": null,
          "cds_length": 1383,
          "cdna_start": 717,
          "cdna_end": null,
          "cdna_length": 7536,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "D",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IDS",
          "gene_hgnc_id": 5389,
          "hgvs_c": "c.592G>A",
          "hgvs_p": "p.Asp198Asn",
          "transcript": "NM_006123.5",
          "protein_id": "NP_006114.1",
          "transcript_support_level": null,
          "aa_start": 198,
          "aa_end": null,
          "aa_length": 343,
          "cds_start": 592,
          "cds_end": null,
          "cds_length": 1032,
          "cdna_start": 761,
          "cdna_end": null,
          "cdna_length": 1358,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IDS",
          "gene_hgnc_id": 5389,
          "hgvs_c": "n.*197G>A",
          "hgvs_p": null,
          "transcript": "ENST00000464251.5",
          "protein_id": "ENSP00000428980.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 959,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IDS",
          "gene_hgnc_id": 5389,
          "hgvs_c": "n.44G>A",
          "hgvs_p": null,
          "transcript": "ENST00000466019.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 537,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IDS",
          "gene_hgnc_id": 5389,
          "hgvs_c": "n.377G>A",
          "hgvs_p": null,
          "transcript": "ENST00000490775.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 945,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IDS",
          "gene_hgnc_id": 5389,
          "hgvs_c": "n.761G>A",
          "hgvs_p": null,
          "transcript": "NR_104128.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1488,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000241489",
          "gene_hgnc_id": null,
          "hgvs_c": "c.-42G>A",
          "hgvs_p": null,
          "transcript": "ENST00000422081.6",
          "protein_id": "ENSP00000477056.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 339,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 1020,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2635,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IDS",
          "gene_hgnc_id": 5389,
          "hgvs_c": "n.*197G>A",
          "hgvs_p": null,
          "transcript": "ENST00000464251.5",
          "protein_id": "ENSP00000428980.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 959,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000241489",
          "gene_hgnc_id": null,
          "hgvs_c": "n.114-11125G>A",
          "hgvs_p": null,
          "transcript": "ENST00000441880.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 416,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "IDS",
      "gene_hgnc_id": 5389,
      "dbsnp": "rs193302904",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.950036346912384,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.899,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9091,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.14,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.344,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 18,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PM1,PM2,PM5,PP3_Strong,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 18,
          "benign_score": 0,
          "pathogenic_score": 18,
          "criteria": [
            "PM1",
            "PM2",
            "PM5",
            "PP3_Strong",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000340855.11",
          "gene_symbol": "IDS",
          "hgnc_id": 5389,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "XL,AR",
          "hgvs_c": "c.592G>A",
          "hgvs_p": "p.Asp198Asn"
        },
        {
          "score": 14,
          "benign_score": 0,
          "pathogenic_score": 14,
          "criteria": [
            "PM2",
            "PP3_Strong",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000651111.1",
          "gene_symbol": "ENSG00000241489",
          "hgnc_id": null,
          "effects": [
            "5_prime_UTR_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.-42G>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": " MPS-II,Mucopolysaccharidosis",
      "clinvar_classification": "Pathogenic/Likely pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:1 LP:2",
      "phenotype_combined": "Mucopolysaccharidosis, MPS-II",
      "pathogenicity_classification_combined": "Pathogenic/Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}