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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: X-22221674-GTT-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=X&pos=22221674&ref=GTT&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "X",
      "pos": 22221674,
      "ref": "GTT",
      "alt": "G",
      "effect": "frameshift_variant",
      "transcript": "ENST00000379374.5",
      "consequences": [
        {
          "aa_ref": "F",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHEX",
          "gene_hgnc_id": 8918,
          "hgvs_c": "c.1832_1833delTT",
          "hgvs_p": "p.Phe611fs",
          "transcript": "NM_000444.6",
          "protein_id": "NP_000435.3",
          "transcript_support_level": null,
          "aa_start": 611,
          "aa_end": null,
          "aa_length": 749,
          "cds_start": 1832,
          "cds_end": null,
          "cds_length": 2250,
          "cdna_start": 2513,
          "cdna_end": null,
          "cdna_length": 6288,
          "mane_select": "ENST00000379374.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHEX",
          "gene_hgnc_id": 8918,
          "hgvs_c": "c.1832_1833delTT",
          "hgvs_p": "p.Phe611fs",
          "transcript": "ENST00000379374.5",
          "protein_id": "ENSP00000368682.4",
          "transcript_support_level": 1,
          "aa_start": 611,
          "aa_end": null,
          "aa_length": 749,
          "cds_start": 1832,
          "cds_end": null,
          "cds_length": 2250,
          "cdna_start": 2513,
          "cdna_end": null,
          "cdna_length": 6288,
          "mane_select": "NM_000444.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHEX",
          "gene_hgnc_id": 8918,
          "hgvs_c": "c.1832_1833delTT",
          "hgvs_p": "p.Phe611fs",
          "transcript": "NM_001282754.2",
          "protein_id": "NP_001269683.1",
          "transcript_support_level": null,
          "aa_start": 611,
          "aa_end": null,
          "aa_length": 695,
          "cds_start": 1832,
          "cds_end": null,
          "cds_length": 2088,
          "cdna_start": 2513,
          "cdna_end": null,
          "cdna_length": 6211,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHEX",
          "gene_hgnc_id": 8918,
          "hgvs_c": "c.386_387delTT",
          "hgvs_p": "p.Phe129fs",
          "transcript": "ENST00000684356.1",
          "protein_id": "ENSP00000507619.1",
          "transcript_support_level": null,
          "aa_start": 129,
          "aa_end": null,
          "aa_length": 267,
          "cds_start": 386,
          "cds_end": null,
          "cds_length": 804,
          "cdna_start": 664,
          "cdna_end": null,
          "cdna_length": 2128,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHEX",
          "gene_hgnc_id": 8918,
          "hgvs_c": "c.386_387delTT",
          "hgvs_p": "p.Phe129fs",
          "transcript": "ENST00000682888.1",
          "protein_id": "ENSP00000508003.1",
          "transcript_support_level": null,
          "aa_start": 129,
          "aa_end": null,
          "aa_length": 170,
          "cds_start": 386,
          "cds_end": null,
          "cds_length": 515,
          "cdna_start": 520,
          "cdna_end": null,
          "cdna_length": 653,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHEX",
          "gene_hgnc_id": 8918,
          "hgvs_c": "c.1541_1542delTT",
          "hgvs_p": "p.Phe514fs",
          "transcript": "XM_024452390.2",
          "protein_id": "XP_024308158.1",
          "transcript_support_level": null,
          "aa_start": 514,
          "aa_end": null,
          "aa_length": 652,
          "cds_start": 1541,
          "cds_end": null,
          "cds_length": 1959,
          "cdna_start": 1954,
          "cdna_end": null,
          "cdna_length": 5729,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHEX",
          "gene_hgnc_id": 8918,
          "hgvs_c": "c.1076_1077delTT",
          "hgvs_p": "p.Phe359fs",
          "transcript": "XM_011545533.2",
          "protein_id": "XP_011543835.1",
          "transcript_support_level": null,
          "aa_start": 359,
          "aa_end": null,
          "aa_length": 497,
          "cds_start": 1076,
          "cds_end": null,
          "cds_length": 1494,
          "cdna_start": 1203,
          "cdna_end": null,
          "cdna_length": 4978,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHEX",
          "gene_hgnc_id": 8918,
          "hgvs_c": "c.1076_1077delTT",
          "hgvs_p": "p.Phe359fs",
          "transcript": "XM_017029579.2",
          "protein_id": "XP_016885068.1",
          "transcript_support_level": null,
          "aa_start": 359,
          "aa_end": null,
          "aa_length": 497,
          "cds_start": 1076,
          "cds_end": null,
          "cds_length": 1494,
          "cdna_start": 1205,
          "cdna_end": null,
          "cdna_length": 4980,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHEX",
          "gene_hgnc_id": 8918,
          "hgvs_c": "c.725_726delTT",
          "hgvs_p": "p.Phe242fs",
          "transcript": "XM_011545536.3",
          "protein_id": "XP_011543838.1",
          "transcript_support_level": null,
          "aa_start": 242,
          "aa_end": null,
          "aa_length": 380,
          "cds_start": 725,
          "cds_end": null,
          "cds_length": 1143,
          "cdna_start": 1223,
          "cdna_end": null,
          "cdna_length": 4998,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHEX",
          "gene_hgnc_id": 8918,
          "hgvs_c": "n.386_387delTT",
          "hgvs_p": null,
          "transcript": "ENST00000683162.1",
          "protein_id": "ENSP00000508059.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2495,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHEX",
          "gene_hgnc_id": 8918,
          "hgvs_c": "n.386_387delTT",
          "hgvs_p": null,
          "transcript": "ENST00000683289.1",
          "protein_id": "ENSP00000508195.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2159,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHEX",
          "gene_hgnc_id": 8918,
          "hgvs_c": "n.616_617delTT",
          "hgvs_p": null,
          "transcript": "ENST00000683917.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1262,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHEX",
          "gene_hgnc_id": 8918,
          "hgvs_c": "n.1506_1507delTT",
          "hgvs_p": null,
          "transcript": "ENST00000684745.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2332,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "PTCHD1-AS",
          "gene_hgnc_id": 37703,
          "hgvs_c": "n.325+5794_325+5795delAA",
          "hgvs_p": null,
          "transcript": "ENST00000669979.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1443,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "PTCHD1-AS",
          "gene_hgnc_id": 37703,
          "hgvs_c": "n.477+5794_477+5795delAA",
          "hgvs_p": null,
          "transcript": "ENST00000715857.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1321,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "PTCHD1-AS",
          "gene_hgnc_id": 37703,
          "hgvs_c": "n.507+5794_507+5795delAA",
          "hgvs_p": null,
          "transcript": "ENST00000825156.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1151,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "PTCHD1-AS",
          "gene_hgnc_id": 37703,
          "hgvs_c": "n.310-32107_310-32106delAA",
          "hgvs_p": null,
          "transcript": "ENST00000825157.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 626,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": 9,
          "intron_rank_end": null,
          "gene_symbol": "PTCHD1-AS",
          "gene_hgnc_id": 37703,
          "hgvs_c": "n.1048+5794_1048+5795delAA",
          "hgvs_p": null,
          "transcript": "NR_073010.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3772,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "PHEX",
      "gene_hgnc_id": 8918,
      "dbsnp": "rs1556138590",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": null,
      "computational_prediction_selected": null,
      "computational_source_selected": null,
      "splice_score_selected": 0.38999998569488525,
      "splice_prediction_selected": "Uncertain_significance",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": null,
      "bayesdelnoaf_prediction": null,
      "phylop100way_score": 7.22,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.39,
      "spliceai_max_prediction": "Uncertain_significance",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 12,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1,PM2,PP5_Moderate",
      "acmg_by_gene": [
        {
          "score": 12,
          "benign_score": 0,
          "pathogenic_score": 12,
          "criteria": [
            "PVS1",
            "PM2",
            "PP5_Moderate"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000379374.5",
          "gene_symbol": "PHEX",
          "hgnc_id": 8918,
          "effects": [
            "frameshift_variant"
          ],
          "inheritance_mode": "XL",
          "hgvs_c": "c.1832_1833delTT",
          "hgvs_p": "p.Phe611fs"
        },
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP5_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NR_073010.2",
          "gene_symbol": "PTCHD1-AS",
          "hgnc_id": 37703,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.1048+5794_1048+5795delAA",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Familial X-linked hypophosphatemic vitamin D refractory rickets",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "P:1",
      "phenotype_combined": "Familial X-linked hypophosphatemic vitamin D refractory rickets",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}