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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: X-38275043-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=X&pos=38275043&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "X",
      "pos": 38275043,
      "ref": "G",
      "alt": "A",
      "effect": "intron_variant",
      "transcript": "ENST00000465127.1",
      "consequences": [
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000250349",
          "gene_hgnc_id": null,
          "hgvs_c": "c.172-391078G>A",
          "hgvs_p": null,
          "transcript": "ENST00000465127.1",
          "protein_id": "ENSP00000417050.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 279,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 840,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 954,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": 16,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.2764+46C>T",
          "hgvs_p": null,
          "transcript": "ENST00000339363.7",
          "protein_id": "ENSP00000343671.3",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1020,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 3063,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3686,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": 17,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.2149+46C>T",
          "hgvs_p": null,
          "transcript": "NM_000328.3",
          "protein_id": "NP_000319.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 815,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2448,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3053,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": 17,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.2149+46C>T",
          "hgvs_p": null,
          "transcript": "ENST00000642395.2",
          "protein_id": "ENSP00000493468.2",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 815,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2448,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3053,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": 17,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.2146+46C>T",
          "hgvs_p": null,
          "transcript": "NM_001367245.1",
          "protein_id": "NP_001354174.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 814,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2445,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3050,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": 16,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1963+46C>T",
          "hgvs_p": null,
          "transcript": "NM_001367246.1",
          "protein_id": "NP_001354175.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 753,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2262,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2867,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": 16,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1963+46C>T",
          "hgvs_p": null,
          "transcript": "ENST00000644337.1",
          "protein_id": "ENSP00000494557.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 753,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2262,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2559,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": 15,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1846+46C>T",
          "hgvs_p": null,
          "transcript": "NM_001367248.1",
          "protein_id": "NP_001354177.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 714,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2145,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2646,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": 15,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1816+46C>T",
          "hgvs_p": null,
          "transcript": "NM_001367247.1",
          "protein_id": "NP_001354176.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 704,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2115,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2720,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": 15,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1813+46C>T",
          "hgvs_p": null,
          "transcript": "NM_001367249.1",
          "protein_id": "NP_001354178.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 703,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2112,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2717,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": null,
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          "exon_count": 17,
          "intron_rank": 15,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1813+46C>T",
          "hgvs_p": null,
          "transcript": "NM_001367250.1",
          "protein_id": "NP_001354179.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 703,
          "cds_start": -4,
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          "cds_length": 2112,
          "cdna_start": null,
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          "cdna_length": 2717,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": null,
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          "exon_count": 16,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1630+46C>T",
          "hgvs_p": null,
          "transcript": "NM_001367251.1",
          "protein_id": "NP_001354180.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": 642,
          "cds_start": -4,
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          "cdna_start": null,
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          "mane_select": null,
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        {
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          "canonical": false,
          "protein_coding": true,
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          "consequences": [
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          "exon_count": 16,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1630+46C>T",
          "hgvs_p": null,
          "transcript": "ENST00000644238.1",
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          "cds_start": -4,
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          "cdna_start": null,
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          "mane_select": null,
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        },
        {
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          ],
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          "intron_rank": 9,
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          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1195+46C>T",
          "hgvs_p": null,
          "transcript": "ENST00000494707.6",
          "protein_id": "ENSP00000417336.2",
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          "cds_start": -4,
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          "cdna_start": null,
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          "mane_select": null,
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          "feature": null
        },
        {
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          "strand": false,
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          ],
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          "exon_count": 16,
          "intron_rank": 14,
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          "gene_symbol": "RPGR",
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          "hgvs_c": "n.*281+46C>T",
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          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
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          "transcript": "ENST00000482855.5",
          "protein_id": "ENSP00000419276.1",
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        },
        {
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          "consequences": [
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          "intron_rank": 15,
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          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "n.2070+46C>T",
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          "transcript": "ENST00000642170.1",
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        {
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          "consequences": [
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          ],
          "exon_rank": null,
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          "exon_count": 18,
          "intron_rank": 15,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "n.1816+46C>T",
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          "gene_symbol": "RPGR",
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          "hgvs_c": "n.*345+46C>T",
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          "gene_symbol": "RPGR",
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          "hgvs_c": "n.*838+46C>T",
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          "transcript": "ENST00000646020.1",
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        },
        {
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          "strand": false,
          "consequences": [
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          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": 19,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "n.2507+46C>T",
          "hgvs_p": null,
          "transcript": "NR_159803.1",
          "protein_id": null,
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          "cdna_length": 3269,
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      ],
      "gene_symbol": "ENSG00000250349",
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      "dbsnp": "rs41312110",
      "frequency_reference_population": 0.048564684,
      "hom_count_reference_population": 13346,
      "allele_count_reference_population": 46781,
      "gnomad_exomes_af": 0.0501779,
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      "gnomad_genomes_ac": 4016,
      "gnomad_exomes_homalt": 914,
      "gnomad_genomes_homalt": 87,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": -0.9700000286102295,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.97,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -1.387,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -20,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BA1",
      "acmg_by_gene": [
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000465127.1",
          "gene_symbol": "ENSG00000250349",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.172-391078G>A",
          "hgvs_p": null
        },
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "NM_000328.3",
          "gene_symbol": "RPGR",
          "hgnc_id": 10295,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "XL,AD",
          "hgvs_c": "c.2149+46C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": " X-linked atrophic,Macular degeneration,Retinitis pigmentosa 3,X-linked cone-rod dystrophy 1,not provided,not specified",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "B:6",
      "phenotype_combined": "not specified|Macular degeneration, X-linked atrophic|Retinitis pigmentosa 3|not provided|X-linked cone-rod dystrophy 1",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}