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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: X-38276743-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=X&pos=38276743&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "X",
      "pos": 38276743,
      "ref": "T",
      "alt": "C",
      "effect": "intron_variant",
      "transcript": "ENST00000465127.1",
      "consequences": [
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000250349",
          "gene_hgnc_id": null,
          "hgvs_c": "c.172-389378T>C",
          "hgvs_p": null,
          "transcript": "ENST00000465127.1",
          "protein_id": "ENSP00000417050.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 279,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 840,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 954,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.2550A>G",
          "hgvs_p": "p.Leu850Leu",
          "transcript": "ENST00000339363.7",
          "protein_id": "ENSP00000343671.3",
          "transcript_support_level": 5,
          "aa_start": 850,
          "aa_end": null,
          "aa_length": 1020,
          "cds_start": 2550,
          "cds_end": null,
          "cds_length": 3063,
          "cdna_start": 2718,
          "cdna_end": null,
          "cdna_length": 3686,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1935A>G",
          "hgvs_p": "p.Leu645Leu",
          "transcript": "NM_000328.3",
          "protein_id": "NP_000319.1",
          "transcript_support_level": null,
          "aa_start": 645,
          "aa_end": null,
          "aa_length": 815,
          "cds_start": 1935,
          "cds_end": null,
          "cds_length": 2448,
          "cdna_start": 2077,
          "cdna_end": null,
          "cdna_length": 3053,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1935A>G",
          "hgvs_p": "p.Leu645Leu",
          "transcript": "ENST00000642395.2",
          "protein_id": "ENSP00000493468.2",
          "transcript_support_level": null,
          "aa_start": 645,
          "aa_end": null,
          "aa_length": 815,
          "cds_start": 1935,
          "cds_end": null,
          "cds_length": 2448,
          "cdna_start": 2077,
          "cdna_end": null,
          "cdna_length": 3053,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1932A>G",
          "hgvs_p": "p.Leu644Leu",
          "transcript": "NM_001367245.1",
          "protein_id": "NP_001354174.1",
          "transcript_support_level": null,
          "aa_start": 644,
          "aa_end": null,
          "aa_length": 814,
          "cds_start": 1932,
          "cds_end": null,
          "cds_length": 2445,
          "cdna_start": 2074,
          "cdna_end": null,
          "cdna_length": 3050,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1749A>G",
          "hgvs_p": "p.Leu583Leu",
          "transcript": "NM_001367246.1",
          "protein_id": "NP_001354175.1",
          "transcript_support_level": null,
          "aa_start": 583,
          "aa_end": null,
          "aa_length": 753,
          "cds_start": 1749,
          "cds_end": null,
          "cds_length": 2262,
          "cdna_start": 1891,
          "cdna_end": null,
          "cdna_length": 2867,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1749A>G",
          "hgvs_p": "p.Leu583Leu",
          "transcript": "ENST00000644337.1",
          "protein_id": "ENSP00000494557.1",
          "transcript_support_level": null,
          "aa_start": 583,
          "aa_end": null,
          "aa_length": 753,
          "cds_start": 1749,
          "cds_end": null,
          "cds_length": 2262,
          "cdna_start": 1917,
          "cdna_end": null,
          "cdna_length": 2559,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1632A>G",
          "hgvs_p": "p.Leu544Leu",
          "transcript": "NM_001367248.1",
          "protein_id": "NP_001354177.1",
          "transcript_support_level": null,
          "aa_start": 544,
          "aa_end": null,
          "aa_length": 714,
          "cds_start": 1632,
          "cds_end": null,
          "cds_length": 2145,
          "cdna_start": 1670,
          "cdna_end": null,
          "cdna_length": 2646,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1602A>G",
          "hgvs_p": "p.Leu534Leu",
          "transcript": "NM_001367247.1",
          "protein_id": "NP_001354176.1",
          "transcript_support_level": null,
          "aa_start": 534,
          "aa_end": null,
          "aa_length": 704,
          "cds_start": 1602,
          "cds_end": null,
          "cds_length": 2115,
          "cdna_start": 1744,
          "cdna_end": null,
          "cdna_length": 2720,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1599A>G",
          "hgvs_p": "p.Leu533Leu",
          "transcript": "NM_001367249.1",
          "protein_id": "NP_001354178.1",
          "transcript_support_level": null,
          "aa_start": 533,
          "aa_end": null,
          "aa_length": 703,
          "cds_start": 1599,
          "cds_end": null,
          "cds_length": 2112,
          "cdna_start": 1741,
          "cdna_end": null,
          "cdna_length": 2717,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1599A>G",
          "hgvs_p": "p.Leu533Leu",
          "transcript": "NM_001367250.1",
          "protein_id": "NP_001354179.1",
          "transcript_support_level": null,
          "aa_start": 533,
          "aa_end": null,
          "aa_length": 703,
          "cds_start": 1599,
          "cds_end": null,
          "cds_length": 2112,
          "cdna_start": 1741,
          "cdna_end": null,
          "cdna_length": 2717,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1416A>G",
          "hgvs_p": "p.Leu472Leu",
          "transcript": "NM_001367251.1",
          "protein_id": "NP_001354180.1",
          "transcript_support_level": null,
          "aa_start": 472,
          "aa_end": null,
          "aa_length": 642,
          "cds_start": 1416,
          "cds_end": null,
          "cds_length": 1929,
          "cdna_start": 1558,
          "cdna_end": null,
          "cdna_length": 2534,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1416A>G",
          "hgvs_p": "p.Leu472Leu",
          "transcript": "ENST00000644238.1",
          "protein_id": "ENSP00000496728.1",
          "transcript_support_level": null,
          "aa_start": 472,
          "aa_end": null,
          "aa_length": 642,
          "cds_start": 1416,
          "cds_end": null,
          "cds_length": 1929,
          "cdna_start": 1564,
          "cdna_end": null,
          "cdna_length": 2301,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.981A>G",
          "hgvs_p": "p.Leu327Leu",
          "transcript": "ENST00000494707.6",
          "protein_id": "ENSP00000417336.2",
          "transcript_support_level": 5,
          "aa_start": 327,
          "aa_end": null,
          "aa_length": 413,
          "cds_start": 981,
          "cds_end": null,
          "cds_length": 1243,
          "cdna_start": 983,
          "cdna_end": null,
          "cdna_length": 1245,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.2550A>G",
          "hgvs_p": "p.Leu850Leu",
          "transcript": "XM_047442329.1",
          "protein_id": "XP_047298285.1",
          "transcript_support_level": null,
          "aa_start": 850,
          "aa_end": null,
          "aa_length": 1020,
          "cds_start": 2550,
          "cds_end": null,
          "cds_length": 3063,
          "cdna_start": 2692,
          "cdna_end": null,
          "cdna_length": 3668,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.2547A>G",
          "hgvs_p": "p.Leu849Leu",
          "transcript": "XM_047442330.1",
          "protein_id": "XP_047298286.1",
          "transcript_support_level": null,
          "aa_start": 849,
          "aa_end": null,
          "aa_length": 1019,
          "cds_start": 2547,
          "cds_end": null,
          "cds_length": 3060,
          "cdna_start": 2689,
          "cdna_end": null,
          "cdna_length": 3665,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "c.1155A>G",
          "hgvs_p": "p.Leu385Leu",
          "transcript": "XM_047442331.1",
          "protein_id": "XP_047298287.1",
          "transcript_support_level": null,
          "aa_start": 385,
          "aa_end": null,
          "aa_length": 555,
          "cds_start": 1155,
          "cds_end": null,
          "cds_length": 1668,
          "cdna_start": 1301,
          "cdna_end": null,
          "cdna_length": 2277,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "n.*67A>G",
          "hgvs_p": null,
          "transcript": "ENST00000474584.5",
          "protein_id": "ENSP00000418926.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2268,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "n.*33A>G",
          "hgvs_p": null,
          "transcript": "ENST00000482855.5",
          "protein_id": "ENSP00000419276.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2819,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "n.1856A>G",
          "hgvs_p": null,
          "transcript": "ENST00000642170.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2872,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPGR",
          "gene_hgnc_id": 10295,
          "hgvs_c": "n.1602A>G",
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      "gene_symbol": "ENSG00000250349",
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      "dbsnp": "rs781266050",
      "frequency_reference_population": 0.000028962864,
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      "gnomad_exomes_af": 0.0000301008,
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      "gnomad_exomes_ac": 33,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.04500000178813934,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "REVEL",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.045,
      "revel_prediction": "Benign",
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.91,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.661,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
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      "apogee2_score": null,
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      "mitotip_score": null,
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      "acmg_score": -14,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Moderate,BP6_Very_Strong,BS2",
      "acmg_by_gene": [
        {
          "score": -14,
          "benign_score": 14,
          "pathogenic_score": 0,
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            "BP6_Very_Strong",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000465127.1",
          "gene_symbol": "ENSG00000250349",
          "hgnc_id": null,
          "effects": [
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          ],
          "inheritance_mode": "",
          "hgvs_c": "c.172-389378T>C",
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        },
        {
          "score": -15,
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            "BP6_Very_Strong",
            "BP7",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_000328.3",
          "gene_symbol": "RPGR",
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          "effects": [
            "synonymous_variant"
          ],
          "inheritance_mode": "XL,AD",
          "hgvs_c": "c.1935A>G",
          "hgvs_p": "p.Leu645Leu"
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      "clinvar_disease": "Primary ciliary dyskinesia",
      "clinvar_classification": "Benign/Likely benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LB:1 B:1",
      "phenotype_combined": "Primary ciliary dyskinesia",
      "pathogenicity_classification_combined": "Benign/Likely benign",
      "custom_annotations": null
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  ],
  "message": null
}