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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: X-41534902-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=X&pos=41534902&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "X",
      "pos": 41534902,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000378163.7",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CASK",
          "gene_hgnc_id": 1497,
          "hgvs_c": "c.2227G>C",
          "hgvs_p": "p.Val743Leu",
          "transcript": "NM_001367721.1",
          "protein_id": "NP_001354650.1",
          "transcript_support_level": null,
          "aa_start": 743,
          "aa_end": null,
          "aa_length": 926,
          "cds_start": 2227,
          "cds_end": null,
          "cds_length": 2781,
          "cdna_start": 2793,
          "cdna_end": null,
          "cdna_length": 8833,
          "mane_select": "ENST00000378163.7",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CASK",
          "gene_hgnc_id": 1497,
          "hgvs_c": "c.2227G>C",
          "hgvs_p": "p.Val743Leu",
          "transcript": "ENST00000378163.7",
          "protein_id": "ENSP00000367405.1",
          "transcript_support_level": 5,
          "aa_start": 743,
          "aa_end": null,
          "aa_length": 926,
          "cds_start": 2227,
          "cds_end": null,
          "cds_length": 2781,
          "cdna_start": 2793,
          "cdna_end": null,
          "cdna_length": 8833,
          "mane_select": "NM_001367721.1",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CASK",
          "gene_hgnc_id": 1497,
          "hgvs_c": "c.2158G>C",
          "hgvs_p": "p.Val720Leu",
          "transcript": "ENST00000421587.8",
          "protein_id": "ENSP00000400526.4",
          "transcript_support_level": 1,
          "aa_start": 720,
          "aa_end": null,
          "aa_length": 903,
          "cds_start": 2158,
          "cds_end": null,
          "cds_length": 2712,
          "cdna_start": 2180,
          "cdna_end": null,
          "cdna_length": 8220,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CASK",
          "gene_hgnc_id": 1497,
          "hgvs_c": "c.2125G>C",
          "hgvs_p": "p.Val709Leu",
          "transcript": "ENST00000378166.9",
          "protein_id": "ENSP00000367408.5",
          "transcript_support_level": 1,
          "aa_start": 709,
          "aa_end": null,
          "aa_length": 892,
          "cds_start": 2125,
          "cds_end": null,
          "cds_length": 2679,
          "cdna_start": 2167,
          "cdna_end": null,
          "cdna_length": 3244,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CASK",
          "gene_hgnc_id": 1497,
          "hgvs_c": "c.2089G>C",
          "hgvs_p": "p.Val697Leu",
          "transcript": "ENST00000442742.7",
          "protein_id": "ENSP00000398007.3",
          "transcript_support_level": 1,
          "aa_start": 697,
          "aa_end": null,
          "aa_length": 880,
          "cds_start": 2089,
          "cds_end": null,
          "cds_length": 2643,
          "cdna_start": 2262,
          "cdna_end": null,
          "cdna_length": 3758,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CASK",
          "gene_hgnc_id": 1497,
          "hgvs_c": "n.1493G>C",
          "hgvs_p": null,
          "transcript": "ENST00000378179.9",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3240,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CASK",
          "gene_hgnc_id": 1497,
          "hgvs_c": "c.2194G>C",
          "hgvs_p": "p.Val732Leu",
          "transcript": "ENST00000645986.2",
          "protein_id": "ENSP00000494409.2",
          "transcript_support_level": null,
          "aa_start": 732,
          "aa_end": null,
          "aa_length": 955,
          "cds_start": 2194,
          "cds_end": null,
          "cds_length": 2868,
          "cdna_start": 2723,
          "cdna_end": null,
          "cdna_length": 4550,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CASK",
          "gene_hgnc_id": 1497,
          "hgvs_c": "c.2230G>C",
          "hgvs_p": "p.Val744Leu",
          "transcript": "ENST00000378168.8",
          "protein_id": "ENSP00000367410.4",
          "transcript_support_level": 5,
          "aa_start": 744,
          "aa_end": null,
          "aa_length": 927,
          "cds_start": 2230,
          "cds_end": null,
          "cds_length": 2784,
          "cdna_start": 2759,
          "cdna_end": null,
          "cdna_length": 8799,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CASK",
          "gene_hgnc_id": 1497,
          "hgvs_c": "c.2212G>C",
          "hgvs_p": "p.Val738Leu",
          "transcript": "NM_003688.4",
          "protein_id": "NP_003679.2",
          "transcript_support_level": null,
          "aa_start": 738,
          "aa_end": null,
          "aa_length": 921,
          "cds_start": 2212,
          "cds_end": null,
          "cds_length": 2766,
          "cdna_start": 2778,
          "cdna_end": null,
          "cdna_length": 8818,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CASK",
          "gene_hgnc_id": 1497,
          "hgvs_c": "c.2212G>C",
          "hgvs_p": "p.Val738Leu",
          "transcript": "ENST00000645566.1",
          "protein_id": "ENSP00000494788.1",
          "transcript_support_level": null,
          "aa_start": 738,
          "aa_end": null,
          "aa_length": 921,
          "cds_start": 2212,
          "cds_end": null,
          "cds_length": 2766,
          "cdna_start": 2239,
          "cdna_end": null,
          "cdna_length": 4002,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
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          "gene_symbol": "CASK",
          "gene_hgnc_id": 1497,
          "hgvs_c": "c.2209G>C",
          "hgvs_p": "p.Val737Leu",
          "transcript": "NM_001410745.1",
          "protein_id": "NP_001397674.1",
          "transcript_support_level": null,
          "aa_start": 737,
          "aa_end": null,
          "aa_length": 920,
          "cds_start": 2209,
          "cds_end": null,
          "cds_length": 2763,
          "cdna_start": 2775,
          "cdna_end": null,
          "cdna_length": 8815,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
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          "exon_count": 26,
          "intron_rank": null,
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          "gene_symbol": "CASK",
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          "hgvs_c": "c.2209G>C",
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          "transcript": "ENST00000644219.1",
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          "transcript_support_level": null,
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          "cdna_start": 2382,
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          "mane_select": null,
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        {
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          "protein_coding": true,
          "strand": false,
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          ],
          "exon_rank": 22,
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          "intron_rank": null,
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          "gene_symbol": "CASK",
          "gene_hgnc_id": 1497,
          "hgvs_c": "c.2176G>C",
          "hgvs_p": "p.Val726Leu",
          "transcript": "ENST00000378154.3",
          "protein_id": "ENSP00000367396.2",
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          "cds_start": 2176,
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          "mane_select": null,
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        {
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          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 21,
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          "intron_rank": null,
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          "gene_symbol": "CASK",
          "gene_hgnc_id": 1497,
          "hgvs_c": "c.2173G>C",
          "hgvs_p": "p.Val725Leu",
          "transcript": "ENST00000378158.6",
          "protein_id": "ENSP00000367400.2",
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          "cds_start": 2173,
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          "cdna_start": 2329,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
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          "protein_coding": true,
          "strand": false,
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          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
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          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
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          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CASK",
          "gene_hgnc_id": 1497,
          "hgvs_c": "c.2143G>C",
          "hgvs_p": "p.Val715Leu",
          "transcript": "NM_001126054.3",
          "protein_id": "NP_001119526.1",
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          "aa_start": 715,
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          "aa_length": 898,
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          "cdna_start": 2709,
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          "feature": null
        },
        {
          "aa_ref": "V",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          "intron_rank": null,
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          "gene_symbol": "CASK",
          "gene_hgnc_id": 1497,
          "hgvs_c": "c.2143G>C",
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          "transcript": "ENST00000646120.2",
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          "cdna_start": 2162,
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        {
          "aa_ref": "V",
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          "gene_symbol": "CASK",
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          "feature": null
        },
        {
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          "strand": false,
          "consequences": [
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          "exon_count": 25,
          "intron_rank": null,
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          "gene_symbol": "CASK",
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          "hgvs_c": "c.2140G>C",
          "hgvs_p": "p.Val714Leu",
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          "cdna_length": 8361,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CASK",
          "gene_hgnc_id": 1497,
          "hgvs_c": "c.1549G>C",
          "hgvs_p": "p.Val517Leu",
          "transcript": "ENST00000646087.2",
          "protein_id": "ENSP00000495510.2",
          "transcript_support_level": null,
          "aa_start": 517,
          "aa_end": null,
          "aa_length": 700,
          "cds_start": 1549,
          "cds_end": null,
          "cds_length": 2103,
          "cdna_start": 1683,
          "cdna_end": null,
          "cdna_length": 3392,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
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      ],
      "gene_symbol": "CASK",
      "gene_hgnc_id": 1497,
      "dbsnp": "rs759074946",
      "frequency_reference_population": 0.00002612044,
      "hom_count_reference_population": 10,
      "allele_count_reference_population": 31,
      "gnomad_exomes_af": 0.0000279203,
      "gnomad_genomes_af": 0.0000089029,
      "gnomad_exomes_ac": 30,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.7608054280281067,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.393,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.9569,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.03,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 7.568,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -11,
      "acmg_classification": "Benign",
      "acmg_criteria": "PP3,BP6_Very_Strong,BS2",
      "acmg_by_gene": [
        {
          "score": -11,
          "benign_score": 12,
          "pathogenic_score": 1,
          "criteria": [
            "PP3",
            "BP6_Very_Strong",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000378163.7",
          "gene_symbol": "CASK",
          "hgnc_id": 1497,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "XL,AD",
          "hgvs_c": "c.2227G>C",
          "hgvs_p": "p.Val743Leu"
        }
      ],
      "clinvar_disease": " CASK-related, X-linked,Intellectual disability,not provided",
      "clinvar_classification": "Benign/Likely benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LB:1 B:1",
      "phenotype_combined": "Intellectual disability, CASK-related, X-linked|not provided",
      "pathogenicity_classification_combined": "Benign/Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}