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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: X-45090821-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=X&pos=45090821&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "X",
      "pos": 45090821,
      "ref": "C",
      "alt": "T",
      "effect": "stop_gained",
      "transcript": "NM_001419809.1",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KDM6A",
          "gene_hgnc_id": 12637,
          "hgvs_c": "c.3991C>T",
          "hgvs_p": "p.Arg1331*",
          "transcript": "NM_001291415.2",
          "protein_id": "NP_001278344.1",
          "transcript_support_level": null,
          "aa_start": 1331,
          "aa_end": null,
          "aa_length": 1453,
          "cds_start": 3991,
          "cds_end": null,
          "cds_length": 4362,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000611820.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001291415.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KDM6A",
          "gene_hgnc_id": 12637,
          "hgvs_c": "c.3991C>T",
          "hgvs_p": "p.Arg1331*",
          "transcript": "ENST00000611820.5",
          "protein_id": "ENSP00000483595.2",
          "transcript_support_level": 1,
          "aa_start": 1331,
          "aa_end": null,
          "aa_length": 1453,
          "cds_start": 3991,
          "cds_end": null,
          "cds_length": 4362,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001291415.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000611820.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KDM6A",
          "gene_hgnc_id": 12637,
          "hgvs_c": "c.3856C>T",
          "hgvs_p": "p.Arg1286*",
          "transcript": "ENST00000382899.9",
          "protein_id": "ENSP00000372355.6",
          "transcript_support_level": 1,
          "aa_start": 1286,
          "aa_end": null,
          "aa_length": 1408,
          "cds_start": 3856,
          "cds_end": null,
          "cds_length": 4227,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000382899.9"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KDM6A",
          "gene_hgnc_id": 12637,
          "hgvs_c": "c.3835C>T",
          "hgvs_p": "p.Arg1279*",
          "transcript": "ENST00000377967.9",
          "protein_id": "ENSP00000367203.4",
          "transcript_support_level": 1,
          "aa_start": 1279,
          "aa_end": null,
          "aa_length": 1401,
          "cds_start": 3835,
          "cds_end": null,
          "cds_length": 4206,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000377967.9"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KDM6A",
          "gene_hgnc_id": 12637,
          "hgvs_c": "c.3700C>T",
          "hgvs_p": "p.Arg1234*",
          "transcript": "ENST00000536777.6",
          "protein_id": "ENSP00000437405.3",
          "transcript_support_level": 1,
          "aa_start": 1234,
          "aa_end": null,
          "aa_length": 1356,
          "cds_start": 3700,
          "cds_end": null,
          "cds_length": 4071,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000536777.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KDM6A",
          "gene_hgnc_id": 12637,
          "hgvs_c": "c.3598C>T",
          "hgvs_p": "p.Arg1200*",
          "transcript": "ENST00000543216.6",
          "protein_id": "ENSP00000443078.3",
          "transcript_support_level": 1,
          "aa_start": 1200,
          "aa_end": null,
          "aa_length": 1322,
          "cds_start": 3598,
          "cds_end": null,
          "cds_length": 3969,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000543216.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KDM6A",
          "gene_hgnc_id": 12637,
          "hgvs_c": "c.3991C>T",
          "hgvs_p": "p.Arg1331*",
          "transcript": "NM_001419809.1",
          "protein_id": "NP_001406738.1",
          "transcript_support_level": null,
          "aa_start": 1331,
          "aa_end": null,
          "aa_length": 1489,
          "cds_start": 3991,
          "cds_end": null,
          "cds_length": 4470,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001419809.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KDM6A",
          "gene_hgnc_id": 12637,
          "hgvs_c": "c.3889C>T",
          "hgvs_p": "p.Arg1297*",
          "transcript": "NM_001419810.1",
          "protein_id": "NP_001406739.1",
          "transcript_support_level": null,
          "aa_start": 1297,
          "aa_end": null,
          "aa_length": 1455,
          "cds_start": 3889,
          "cds_end": null,
          "cds_length": 4368,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001419810.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KDM6A",
          "gene_hgnc_id": 12637,
          "hgvs_c": "c.3856C>T",
          "hgvs_p": "p.Arg1286*",
          "transcript": "NM_001419811.1",
          "protein_id": "NP_001406740.1",
          "transcript_support_level": null,
          "aa_start": 1286,
          "aa_end": null,
          "aa_length": 1444,
          "cds_start": 3856,
          "cds_end": null,
          "cds_length": 4335,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001419811.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KDM6A",
          "gene_hgnc_id": 12637,
          "hgvs_c": "c.3835C>T",
          "hgvs_p": "p.Arg1279*",
          "transcript": "NM_001419812.1",
          "protein_id": "NP_001406741.1",
          "transcript_support_level": null,
          "aa_start": 1279,
          "aa_end": null,
          "aa_length": 1437,
          "cds_start": 3835,
          "cds_end": null,
          "cds_length": 4314,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001419812.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KDM6A",
          "gene_hgnc_id": 12637,
          "hgvs_c": "c.3913C>T",
          "hgvs_p": "p.Arg1305*",
          "transcript": "ENST00000674586.1",
          "protein_id": "ENSP00000502660.1",
          "transcript_support_level": null,
          "aa_start": 1305,
          "aa_end": null,
          "aa_length": 1427,
          "cds_start": 3913,
          "cds_end": null,
          "cds_length": 4284,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000674586.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KDM6A",
          "gene_hgnc_id": 12637,
          "hgvs_c": "c.3889C>T",
          "hgvs_p": "p.Arg1297*",
          "transcript": "NM_001419813.1",
          "protein_id": "NP_001406742.1",
          "transcript_support_level": null,
          "aa_start": 1297,
          "aa_end": null,
          "aa_length": 1419,
          "cds_start": 3889,
          "cds_end": null,
          "cds_length": 4260,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001419813.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KDM6A",
          "gene_hgnc_id": 12637,
          "hgvs_c": "c.3889C>T",
          "hgvs_p": "p.Arg1297*",
          "transcript": "ENST00000867282.1",
          "protein_id": "ENSP00000537341.1",
          "transcript_support_level": null,
          "aa_start": 1297,
          "aa_end": null,
          "aa_length": 1419,
          "cds_start": 3889,
          "cds_end": null,
          "cds_length": 4260,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867282.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KDM6A",
          "gene_hgnc_id": 12637,
          "hgvs_c": "c.3856C>T",
          "hgvs_p": "p.Arg1286*",
          "transcript": "NM_001291416.2",
          "protein_id": "NP_001278345.1",
          "transcript_support_level": null,
          "aa_start": 1286,
          "aa_end": null,
          "aa_length": 1408,
          "cds_start": 3856,
          "cds_end": null,
          "cds_length": 4227,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001291416.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KDM6A",
          "gene_hgnc_id": 12637,
          "hgvs_c": "c.3733C>T",
          "hgvs_p": "p.Arg1245*",
          "transcript": "NM_001419814.1",
          "protein_id": "NP_001406743.1",
          "transcript_support_level": null,
          "aa_start": 1245,
          "aa_end": null,
          "aa_length": 1403,
          "cds_start": 3733,
          "cds_end": null,
          "cds_length": 4212,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001419814.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KDM6A",
          "gene_hgnc_id": 12637,
          "hgvs_c": "c.3835C>T",
          "hgvs_p": "p.Arg1279*",
          "transcript": "NM_021140.4",
          "protein_id": "NP_066963.2",
          "transcript_support_level": null,
          "aa_start": 1279,
          "aa_end": null,
          "aa_length": 1401,
          "cds_start": 3835,
          "cds_end": null,
          "cds_length": 4206,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_021140.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KDM6A",
          "gene_hgnc_id": 12637,
          "hgvs_c": "c.3829C>T",
          "hgvs_p": "p.Arg1277*",
          "transcript": "ENST00000867283.1",
          "protein_id": "ENSP00000537342.1",
          "transcript_support_level": null,
          "aa_start": 1277,
          "aa_end": null,
          "aa_length": 1399,
          "cds_start": 3829,
          "cds_end": null,
          "cds_length": 4200,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867283.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KDM6A",
          "gene_hgnc_id": 12637,
          "hgvs_c": "c.3754C>T",
          "hgvs_p": "p.Arg1252*",
          "transcript": "ENST00000683021.1",
          "protein_id": "ENSP00000507416.1",
          "transcript_support_level": null,
          "aa_start": 1252,
          "aa_end": null,
          "aa_length": 1374,
          "cds_start": 3754,
          "cds_end": null,
          "cds_length": 4125,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000683021.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KDM6A",
          "gene_hgnc_id": 12637,
          "hgvs_c": "c.3733C>T",
          "hgvs_p": "p.Arg1245*",
          "transcript": "NM_001410742.1",
          "protein_id": "NP_001397671.1",
          "transcript_support_level": null,
          "aa_start": 1245,
          "aa_end": null,
          "aa_length": 1367,
          "cds_start": 3733,
          "cds_end": null,
          "cds_length": 4104,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001410742.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KDM6A",
          "gene_hgnc_id": 12637,
          "hgvs_c": "c.3733C>T",
          "hgvs_p": "p.Arg1245*",
          "transcript": "ENST00000675577.1",
          "protein_id": "ENSP00000501855.1",
          "transcript_support_level": null,
          "aa_start": 1245,
          "aa_end": null,
          "aa_length": 1367,
          "cds_start": 3733,
          "cds_end": null,
          "cds_length": 4104,
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          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000676343.1"
        }
      ],
      "gene_symbol": "KDM6A",
      "gene_hgnc_id": 12637,
      "dbsnp": "rs863224886",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.09099999815225601,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "REVEL",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.091,
      "revel_prediction": "Benign",
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.63,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 3.513,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 18,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1,PM2,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 18,
          "benign_score": 0,
          "pathogenic_score": 18,
          "criteria": [
            "PVS1",
            "PM2",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "NM_001419809.1",
          "gene_symbol": "KDM6A",
          "hgnc_id": 12637,
          "effects": [
            "stop_gained"
          ],
          "inheritance_mode": "XL,AD",
          "hgvs_c": "c.3991C>T",
          "hgvs_p": "p.Arg1331*"
        }
      ],
      "clinvar_disease": "Kabuki syndrome 2,not provided",
      "clinvar_classification": "Pathogenic/Likely pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:2 LP:1",
      "phenotype_combined": "Kabuki syndrome 2|not provided",
      "pathogenicity_classification_combined": "Pathogenic/Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}