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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: X-49076468-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=X&pos=49076468&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "X",
      "pos": 49076468,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "ENST00000376372.9",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WDR45",
          "gene_hgnc_id": 28912,
          "hgvs_c": "c.398G>A",
          "hgvs_p": "p.Arg133Gln",
          "transcript": "NM_001029896.2",
          "protein_id": "NP_001025067.1",
          "transcript_support_level": null,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 360,
          "cds_start": 398,
          "cds_end": null,
          "cds_length": 1083,
          "cdna_start": 552,
          "cdna_end": null,
          "cdna_length": 1598,
          "mane_select": "ENST00000376372.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WDR45",
          "gene_hgnc_id": 28912,
          "hgvs_c": "c.398G>A",
          "hgvs_p": "p.Arg133Gln",
          "transcript": "ENST00000376372.9",
          "protein_id": "ENSP00000365551.3",
          "transcript_support_level": 1,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 360,
          "cds_start": 398,
          "cds_end": null,
          "cds_length": 1083,
          "cdna_start": 552,
          "cdna_end": null,
          "cdna_length": 1598,
          "mane_select": "NM_001029896.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WDR45",
          "gene_hgnc_id": 28912,
          "hgvs_c": "c.401G>A",
          "hgvs_p": "p.Arg134Gln",
          "transcript": "ENST00000356463.7",
          "protein_id": "ENSP00000348848.3",
          "transcript_support_level": 1,
          "aa_start": 134,
          "aa_end": null,
          "aa_length": 361,
          "cds_start": 401,
          "cds_end": null,
          "cds_length": 1086,
          "cdna_start": 840,
          "cdna_end": null,
          "cdna_length": 1688,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WDR45",
          "gene_hgnc_id": 28912,
          "hgvs_c": "c.401G>A",
          "hgvs_p": "p.Arg134Gln",
          "transcript": "ENST00000376368.7",
          "protein_id": "ENSP00000365546.2",
          "transcript_support_level": 1,
          "aa_start": 134,
          "aa_end": null,
          "aa_length": 361,
          "cds_start": 401,
          "cds_end": null,
          "cds_length": 1086,
          "cdna_start": 597,
          "cdna_end": null,
          "cdna_length": 1381,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000288053",
          "gene_hgnc_id": null,
          "hgvs_c": "c.131-523G>A",
          "hgvs_p": null,
          "transcript": "ENST00000376358.4",
          "protein_id": "ENSP00000365536.3",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 292,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 879,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1432,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WDR45",
          "gene_hgnc_id": 28912,
          "hgvs_c": "c.398G>A",
          "hgvs_p": "p.Arg133Gln",
          "transcript": "ENST00000322995.13",
          "protein_id": "ENSP00000365543.5",
          "transcript_support_level": 5,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 371,
          "cds_start": 398,
          "cds_end": null,
          "cds_length": 1116,
          "cdna_start": 563,
          "cdna_end": null,
          "cdna_length": 1444,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WDR45",
          "gene_hgnc_id": 28912,
          "hgvs_c": "c.401G>A",
          "hgvs_p": "p.Arg134Gln",
          "transcript": "NM_007075.4",
          "protein_id": "NP_009006.2",
          "transcript_support_level": null,
          "aa_start": 134,
          "aa_end": null,
          "aa_length": 361,
          "cds_start": 401,
          "cds_end": null,
          "cds_length": 1086,
          "cdna_start": 897,
          "cdna_end": null,
          "cdna_length": 1943,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WDR45",
          "gene_hgnc_id": 28912,
          "hgvs_c": "c.398G>A",
          "hgvs_p": "p.Arg133Gln",
          "transcript": "ENST00000634944.1",
          "protein_id": "ENSP00000488972.1",
          "transcript_support_level": 5,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 360,
          "cds_start": 398,
          "cds_end": null,
          "cds_length": 1083,
          "cdna_start": 699,
          "cdna_end": null,
          "cdna_length": 1745,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WDR45",
          "gene_hgnc_id": 28912,
          "hgvs_c": "c.398G>A",
          "hgvs_p": "p.Arg133Gln",
          "transcript": "ENST00000634838.1",
          "protein_id": "ENSP00000489268.1",
          "transcript_support_level": 5,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 346,
          "cds_start": 398,
          "cds_end": null,
          "cds_length": 1041,
          "cdna_start": 546,
          "cdna_end": null,
          "cdna_length": 1545,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WDR45",
          "gene_hgnc_id": 28912,
          "hgvs_c": "c.293G>A",
          "hgvs_p": "p.Arg98Gln",
          "transcript": "ENST00000485908.6",
          "protein_id": "ENSP00000419897.1",
          "transcript_support_level": 5,
          "aa_start": 98,
          "aa_end": null,
          "aa_length": 325,
          "cds_start": 293,
          "cds_end": null,
          "cds_length": 978,
          "cdna_start": 471,
          "cdna_end": null,
          "cdna_length": 1301,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WDR45",
          "gene_hgnc_id": 28912,
          "hgvs_c": "c.293G>A",
          "hgvs_p": "p.Arg98Gln",
          "transcript": "ENST00000396681.9",
          "protein_id": "ENSP00000379913.5",
          "transcript_support_level": 5,
          "aa_start": 98,
          "aa_end": null,
          "aa_length": 321,
          "cds_start": 293,
          "cds_end": null,
          "cds_length": 966,
          "cdna_start": 462,
          "cdna_end": null,
          "cdna_length": 1146,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WDR45",
          "gene_hgnc_id": 28912,
          "hgvs_c": "c.266G>A",
          "hgvs_p": "p.Arg89Gln",
          "transcript": "ENST00000367375.8",
          "protein_id": "ENSP00000356345.4",
          "transcript_support_level": 5,
          "aa_start": 89,
          "aa_end": null,
          "aa_length": 286,
          "cds_start": 266,
          "cds_end": null,
          "cds_length": 861,
          "cdna_start": 267,
          "cdna_end": null,
          "cdna_length": 996,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WDR45",
          "gene_hgnc_id": 28912,
          "hgvs_c": "c.326G>A",
          "hgvs_p": "p.Arg109Gln",
          "transcript": "ENST00000635666.1",
          "protein_id": "ENSP00000489128.1",
          "transcript_support_level": 5,
          "aa_start": 109,
          "aa_end": null,
          "aa_length": 275,
          "cds_start": 326,
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          "cds_length": 830,
          "cdna_start": 443,
          "cdna_end": null,
          "cdna_length": 947,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WDR45",
          "gene_hgnc_id": 28912,
          "hgvs_c": "c.398G>A",
          "hgvs_p": "p.Arg133Gln",
          "transcript": "ENST00000473974.5",
          "protein_id": "ENSP00000417211.1",
          "transcript_support_level": 5,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 256,
          "cds_start": 398,
          "cds_end": null,
          "cds_length": 771,
          "cdna_start": 537,
          "cdna_end": null,
          "cdna_length": 1077,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "WDR45",
          "gene_hgnc_id": 28912,
          "hgvs_c": "c.296G>A",
          "hgvs_p": "p.Arg99Gln",
          "transcript": "ENST00000475880.6",
          "protein_id": "ENSP00000418919.2",
          "transcript_support_level": 5,
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          "cds_start": 296,
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          "cdna_start": 443,
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          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WDR45",
          "gene_hgnc_id": 28912,
          "hgvs_c": "c.473G>A",
          "hgvs_p": "p.Arg158Gln",
          "transcript": "ENST00000474053.6",
          "protein_id": "ENSP00000420728.1",
          "transcript_support_level": 5,
          "aa_start": 158,
          "aa_end": null,
          "aa_length": 212,
          "cds_start": 473,
          "cds_end": null,
          "cds_length": 641,
          "cdna_start": 560,
          "cdna_end": null,
          "cdna_length": 728,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
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          "gene_symbol": "WDR45",
          "gene_hgnc_id": 28912,
          "hgvs_c": "c.398G>A",
          "hgvs_p": "p.Arg133Gln",
          "transcript": "ENST00000419567.7",
          "protein_id": "ENSP00000393640.3",
          "transcript_support_level": 5,
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          "aa_length": 207,
          "cds_start": 398,
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          "cds_length": 626,
          "cdna_start": 415,
          "cdna_end": null,
          "cdna_length": 643,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
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          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WDR45",
          "gene_hgnc_id": 28912,
          "hgvs_c": "c.452G>A",
          "hgvs_p": "p.Arg151Gln",
          "transcript": "ENST00000423215.3",
          "protein_id": "ENSP00000397657.3",
          "transcript_support_level": 5,
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          "cdna_start": 684,
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          "biotype": null,
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        },
        {
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
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          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "WDR45",
          "gene_hgnc_id": 28912,
          "hgvs_c": "c.398G>A",
          "hgvs_p": "p.Arg133Gln",
          "transcript": "ENST00000471338.6",
          "protein_id": "ENSP00000418466.2",
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          "mane_select": null,
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        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 6,
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          "intron_rank": null,
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          "gene_symbol": "WDR45",
          "gene_hgnc_id": 28912,
          "hgvs_c": "c.467G>A",
          "hgvs_p": "p.Arg156Gln",
          "transcript": "ENST00000634849.1",
          "protein_id": "ENSP00000489150.1",
          "transcript_support_level": 3,
          "aa_start": 156,
          "aa_end": null,
          "aa_length": 161,
          "cds_start": 467,
          "cds_end": null,
          "cds_length": 488,
          "cdna_start": 897,
          "cdna_end": null,
          "cdna_length": 918,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WDR45",
          "gene_hgnc_id": 28912,
          "hgvs_c": "c.398G>A",
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      ],
      "gene_symbol": "WDR45",
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      "dbsnp": "rs144081442",
      "frequency_reference_population": 0.000019000618,
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      "gnomad_exomes_af": 0.0000154809,
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      "gnomad_exomes_homalt": 0,
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      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.08614414930343628,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.19,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0701,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.45,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.779,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
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      "apogee2_score": null,
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      "mitotip_score": null,
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      "acmg_score": -8,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Moderate,BP6_Moderate,BS2",
      "acmg_by_gene": [
        {
          "score": -8,
          "benign_score": 8,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BP6_Moderate",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000376372.9",
          "gene_symbol": "WDR45",
          "hgnc_id": 28912,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "XL,AD",
          "hgvs_c": "c.398G>A",
          "hgvs_p": "p.Arg133Gln"
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        {
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          "pathogenic_score": 0,
          "criteria": [
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            "BP6_Moderate",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000376358.4",
          "gene_symbol": "ENSG00000288053",
          "hgnc_id": null,
          "effects": [
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          ],
          "inheritance_mode": "",
          "hgvs_c": "c.131-523G>A",
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      ],
      "clinvar_disease": "Neurodegeneration with brain iron accumulation 5",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LB:1",
      "phenotype_combined": "Neurodegeneration with brain iron accumulation 5",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}