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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: X-50090127-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=X&pos=50090127&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "X",
      "pos": 50090127,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "ENST00000376091.8",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLCN5",
          "gene_hgnc_id": 2023,
          "hgvs_c": "c.1756C>T",
          "hgvs_p": "p.Arg586Trp",
          "transcript": "NM_001127898.4",
          "protein_id": "NP_001121370.1",
          "transcript_support_level": null,
          "aa_start": 586,
          "aa_end": null,
          "aa_length": 816,
          "cds_start": 1756,
          "cds_end": null,
          "cds_length": 2451,
          "cdna_start": 2157,
          "cdna_end": null,
          "cdna_length": 9863,
          "mane_select": "ENST00000376091.8",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLCN5",
          "gene_hgnc_id": 2023,
          "hgvs_c": "c.1756C>T",
          "hgvs_p": "p.Arg586Trp",
          "transcript": "ENST00000376091.8",
          "protein_id": "ENSP00000365259.3",
          "transcript_support_level": 2,
          "aa_start": 586,
          "aa_end": null,
          "aa_length": 816,
          "cds_start": 1756,
          "cds_end": null,
          "cds_length": 2451,
          "cdna_start": 2157,
          "cdna_end": null,
          "cdna_length": 9863,
          "mane_select": "NM_001127898.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLCN5",
          "gene_hgnc_id": 2023,
          "hgvs_c": "c.1546C>T",
          "hgvs_p": "p.Arg516Trp",
          "transcript": "ENST00000307367.2",
          "protein_id": "ENSP00000304257.2",
          "transcript_support_level": 1,
          "aa_start": 516,
          "aa_end": null,
          "aa_length": 746,
          "cds_start": 1546,
          "cds_end": null,
          "cds_length": 2241,
          "cdna_start": 1837,
          "cdna_end": null,
          "cdna_length": 3177,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLCN5",
          "gene_hgnc_id": 2023,
          "hgvs_c": "c.1546C>T",
          "hgvs_p": "p.Arg516Trp",
          "transcript": "ENST00000376108.7",
          "protein_id": "ENSP00000365276.3",
          "transcript_support_level": 1,
          "aa_start": 516,
          "aa_end": null,
          "aa_length": 746,
          "cds_start": 1546,
          "cds_end": null,
          "cds_length": 2241,
          "cdna_start": 1778,
          "cdna_end": null,
          "cdna_length": 2660,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLCN5",
          "gene_hgnc_id": 2023,
          "hgvs_c": "c.1768C>T",
          "hgvs_p": "p.Arg590Trp",
          "transcript": "NM_001440756.1",
          "protein_id": "NP_001427685.1",
          "transcript_support_level": null,
          "aa_start": 590,
          "aa_end": null,
          "aa_length": 820,
          "cds_start": 1768,
          "cds_end": null,
          "cds_length": 2463,
          "cdna_start": 2169,
          "cdna_end": null,
          "cdna_length": 9875,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLCN5",
          "gene_hgnc_id": 2023,
          "hgvs_c": "c.1768C>T",
          "hgvs_p": "p.Arg590Trp",
          "transcript": "NM_001440757.1",
          "protein_id": "NP_001427686.1",
          "transcript_support_level": null,
          "aa_start": 590,
          "aa_end": null,
          "aa_length": 820,
          "cds_start": 1768,
          "cds_end": null,
          "cds_length": 2463,
          "cdna_start": 2428,
          "cdna_end": null,
          "cdna_length": 10134,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLCN5",
          "gene_hgnc_id": 2023,
          "hgvs_c": "c.1756C>T",
          "hgvs_p": "p.Arg586Trp",
          "transcript": "NM_001127899.4",
          "protein_id": "NP_001121371.1",
          "transcript_support_level": null,
          "aa_start": 586,
          "aa_end": null,
          "aa_length": 816,
          "cds_start": 1756,
          "cds_end": null,
          "cds_length": 2451,
          "cdna_start": 2416,
          "cdna_end": null,
          "cdna_length": 10122,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLCN5",
          "gene_hgnc_id": 2023,
          "hgvs_c": "c.1756C>T",
          "hgvs_p": "p.Arg586Trp",
          "transcript": "ENST00000376088.7",
          "protein_id": "ENSP00000365256.3",
          "transcript_support_level": 2,
          "aa_start": 586,
          "aa_end": null,
          "aa_length": 816,
          "cds_start": 1756,
          "cds_end": null,
          "cds_length": 2451,
          "cdna_start": 2397,
          "cdna_end": null,
          "cdna_length": 10108,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLCN5",
          "gene_hgnc_id": 2023,
          "hgvs_c": "c.1606C>T",
          "hgvs_p": "p.Arg536Trp",
          "transcript": "NM_001282163.2",
          "protein_id": "NP_001269092.1",
          "transcript_support_level": null,
          "aa_start": 536,
          "aa_end": null,
          "aa_length": 766,
          "cds_start": 1606,
          "cds_end": null,
          "cds_length": 2301,
          "cdna_start": 1837,
          "cdna_end": null,
          "cdna_length": 9543,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLCN5",
          "gene_hgnc_id": 2023,
          "hgvs_c": "c.1546C>T",
          "hgvs_p": "p.Arg516Trp",
          "transcript": "NM_000084.5",
          "protein_id": "NP_000075.1",
          "transcript_support_level": null,
          "aa_start": 516,
          "aa_end": null,
          "aa_length": 746,
          "cds_start": 1546,
          "cds_end": null,
          "cds_length": 2241,
          "cdna_start": 1766,
          "cdna_end": null,
          "cdna_length": 9472,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLCN5",
          "gene_hgnc_id": 2023,
          "hgvs_c": "c.1546C>T",
          "hgvs_p": "p.Arg516Trp",
          "transcript": "ENST00000642885.1",
          "protein_id": "ENSP00000496632.1",
          "transcript_support_level": null,
          "aa_start": 516,
          "aa_end": null,
          "aa_length": 746,
          "cds_start": 1546,
          "cds_end": null,
          "cds_length": 2241,
          "cdna_start": 1698,
          "cdna_end": null,
          "cdna_length": 2834,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLCN5",
          "gene_hgnc_id": 2023,
          "hgvs_c": "c.1078C>T",
          "hgvs_p": "p.Arg360Trp",
          "transcript": "ENST00000642383.1",
          "protein_id": "ENSP00000496353.1",
          "transcript_support_level": null,
          "aa_start": 360,
          "aa_end": null,
          "aa_length": 590,
          "cds_start": 1078,
          "cds_end": null,
          "cds_length": 1773,
          "cdna_start": 1078,
          "cdna_end": null,
          "cdna_length": 2214,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLCN5",
          "gene_hgnc_id": 2023,
          "hgvs_c": "c.1546C>T",
          "hgvs_p": "p.Arg516Trp",
          "transcript": "XM_047441807.1",
          "protein_id": "XP_047297763.1",
          "transcript_support_level": null,
          "aa_start": 516,
          "aa_end": null,
          "aa_length": 746,
          "cds_start": 1546,
          "cds_end": null,
          "cds_length": 2241,
          "cdna_start": 1808,
          "cdna_end": null,
          "cdna_length": 9514,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLCN5",
          "gene_hgnc_id": 2023,
          "hgvs_c": "c.1546C>T",
          "hgvs_p": "p.Arg516Trp",
          "transcript": "XM_047441808.1",
          "protein_id": "XP_047297764.1",
          "transcript_support_level": null,
          "aa_start": 516,
          "aa_end": null,
          "aa_length": 746,
          "cds_start": 1546,
          "cds_end": null,
          "cds_length": 2241,
          "cdna_start": 1863,
          "cdna_end": null,
          "cdna_length": 9569,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLCN5",
          "gene_hgnc_id": 2023,
          "hgvs_c": "n.*1853C>T",
          "hgvs_p": null,
          "transcript": "ENST00000643129.1",
          "protein_id": "ENSP00000496056.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3179,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLCN5",
          "gene_hgnc_id": 2023,
          "hgvs_c": "n.*931C>T",
          "hgvs_p": null,
          "transcript": "ENST00000646398.1",
          "protein_id": "ENSP00000495122.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3470,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLCN5",
          "gene_hgnc_id": 2023,
          "hgvs_c": "n.2852C>T",
          "hgvs_p": null,
          "transcript": "NR_199781.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 10558,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLCN5",
          "gene_hgnc_id": 2023,
          "hgvs_c": "n.*1853C>T",
          "hgvs_p": null,
          "transcript": "ENST00000643129.1",
          "protein_id": "ENSP00000496056.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3179,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CLCN5",
          "gene_hgnc_id": 2023,
          "hgvs_c": "n.*931C>T",
          "hgvs_p": null,
          "transcript": "ENST00000646398.1",
          "protein_id": "ENSP00000495122.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3470,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "CLCN5",
      "gene_hgnc_id": 2023,
      "dbsnp": "rs797044812",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9883052110671997,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.94,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9843,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.68,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 1.405,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 16,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PM1,PM2,PP3_Strong,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 16,
          "benign_score": 0,
          "pathogenic_score": 16,
          "criteria": [
            "PM1",
            "PM2",
            "PP3_Strong",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000376091.8",
          "gene_symbol": "CLCN5",
          "hgnc_id": 2023,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "XL",
          "hgvs_c": "c.1756C>T",
          "hgvs_p": "p.Arg586Trp"
        }
      ],
      "clinvar_disease": " X-linked recessive, low molecular weight, with hypercalciuria and nephrocalcinosis,Dent disease type 1,Hypophosphatemic rickets,Proteinuria,X-linked recessive nephrolithiasis with renal failure",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LP:2 O:1",
      "phenotype_combined": "Dent disease type 1|X-linked recessive nephrolithiasis with renal failure;Dent disease type 1;Hypophosphatemic rickets, X-linked recessive;Proteinuria, low molecular weight, with hypercalciuria and nephrocalcinosis",
      "pathogenicity_classification_combined": "Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}