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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: X-54814725-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=X&pos=54814725&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "X",
      "pos": 54814725,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_014599.6",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAGED2",
          "gene_hgnc_id": 16353,
          "hgvs_c": "c.1336C>T",
          "hgvs_p": "p.Arg446Cys",
          "transcript": "NM_177433.3",
          "protein_id": "NP_803182.1",
          "transcript_support_level": null,
          "aa_start": 446,
          "aa_end": null,
          "aa_length": 606,
          "cds_start": 1336,
          "cds_end": null,
          "cds_length": 1821,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000375068.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_177433.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAGED2",
          "gene_hgnc_id": 16353,
          "hgvs_c": "c.1336C>T",
          "hgvs_p": "p.Arg446Cys",
          "transcript": "ENST00000375068.6",
          "protein_id": "ENSP00000364209.1",
          "transcript_support_level": 1,
          "aa_start": 446,
          "aa_end": null,
          "aa_length": 606,
          "cds_start": 1336,
          "cds_end": null,
          "cds_length": 1821,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_177433.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000375068.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAGED2",
          "gene_hgnc_id": 16353,
          "hgvs_c": "c.1336C>T",
          "hgvs_p": "p.Arg446Cys",
          "transcript": "ENST00000375053.6",
          "protein_id": "ENSP00000364193.2",
          "transcript_support_level": 1,
          "aa_start": 446,
          "aa_end": null,
          "aa_length": 606,
          "cds_start": 1336,
          "cds_end": null,
          "cds_length": 1821,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000375053.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAGED2",
          "gene_hgnc_id": 16353,
          "hgvs_c": "c.1336C>T",
          "hgvs_p": "p.Arg446Cys",
          "transcript": "ENST00000375058.5",
          "protein_id": "ENSP00000364198.1",
          "transcript_support_level": 1,
          "aa_start": 446,
          "aa_end": null,
          "aa_length": 606,
          "cds_start": 1336,
          "cds_end": null,
          "cds_length": 1821,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000375058.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAGED2",
          "gene_hgnc_id": 16353,
          "hgvs_c": "c.1441C>T",
          "hgvs_p": "p.Arg481Cys",
          "transcript": "ENST00000919666.1",
          "protein_id": "ENSP00000589725.1",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 641,
          "cds_start": 1441,
          "cds_end": null,
          "cds_length": 1926,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919666.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAGED2",
          "gene_hgnc_id": 16353,
          "hgvs_c": "c.1438C>T",
          "hgvs_p": "p.Arg480Cys",
          "transcript": "ENST00000872302.1",
          "protein_id": "ENSP00000542361.1",
          "transcript_support_level": null,
          "aa_start": 480,
          "aa_end": null,
          "aa_length": 640,
          "cds_start": 1438,
          "cds_end": null,
          "cds_length": 1923,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872302.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAGED2",
          "gene_hgnc_id": 16353,
          "hgvs_c": "c.1438C>T",
          "hgvs_p": "p.Arg480Cys",
          "transcript": "ENST00000944666.1",
          "protein_id": "ENSP00000614725.1",
          "transcript_support_level": null,
          "aa_start": 480,
          "aa_end": null,
          "aa_length": 640,
          "cds_start": 1438,
          "cds_end": null,
          "cds_length": 1923,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000944666.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAGED2",
          "gene_hgnc_id": 16353,
          "hgvs_c": "c.1420C>T",
          "hgvs_p": "p.Arg474Cys",
          "transcript": "ENST00000872307.1",
          "protein_id": "ENSP00000542366.1",
          "transcript_support_level": null,
          "aa_start": 474,
          "aa_end": null,
          "aa_length": 634,
          "cds_start": 1420,
          "cds_end": null,
          "cds_length": 1905,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872307.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAGED2",
          "gene_hgnc_id": 16353,
          "hgvs_c": "c.1420C>T",
          "hgvs_p": "p.Arg474Cys",
          "transcript": "ENST00000919654.1",
          "protein_id": "ENSP00000589713.1",
          "transcript_support_level": null,
          "aa_start": 474,
          "aa_end": null,
          "aa_length": 634,
          "cds_start": 1420,
          "cds_end": null,
          "cds_length": 1905,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919654.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAGED2",
          "gene_hgnc_id": 16353,
          "hgvs_c": "c.1420C>T",
          "hgvs_p": "p.Arg474Cys",
          "transcript": "ENST00000919667.1",
          "protein_id": "ENSP00000589726.1",
          "transcript_support_level": null,
          "aa_start": 474,
          "aa_end": null,
          "aa_length": 634,
          "cds_start": 1420,
          "cds_end": null,
          "cds_length": 1905,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919667.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAGED2",
          "gene_hgnc_id": 16353,
          "hgvs_c": "c.1408C>T",
          "hgvs_p": "p.Arg470Cys",
          "transcript": "ENST00000919661.1",
          "protein_id": "ENSP00000589720.1",
          "transcript_support_level": null,
          "aa_start": 470,
          "aa_end": null,
          "aa_length": 630,
          "cds_start": 1408,
          "cds_end": null,
          "cds_length": 1893,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919661.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAGED2",
          "gene_hgnc_id": 16353,
          "hgvs_c": "c.1408C>T",
          "hgvs_p": "p.Arg470Cys",
          "transcript": "ENST00000944667.1",
          "protein_id": "ENSP00000614726.1",
          "transcript_support_level": null,
          "aa_start": 470,
          "aa_end": null,
          "aa_length": 630,
          "cds_start": 1408,
          "cds_end": null,
          "cds_length": 1893,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000944667.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAGED2",
          "gene_hgnc_id": 16353,
          "hgvs_c": "c.1381C>T",
          "hgvs_p": "p.Arg461Cys",
          "transcript": "ENST00000872297.1",
          "protein_id": "ENSP00000542356.1",
          "transcript_support_level": null,
          "aa_start": 461,
          "aa_end": null,
          "aa_length": 621,
          "cds_start": 1381,
          "cds_end": null,
          "cds_length": 1866,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872297.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAGED2",
          "gene_hgnc_id": 16353,
          "hgvs_c": "c.1381C>T",
          "hgvs_p": "p.Arg461Cys",
          "transcript": "ENST00000872308.1",
          "protein_id": "ENSP00000542367.1",
          "transcript_support_level": null,
          "aa_start": 461,
          "aa_end": null,
          "aa_length": 621,
          "cds_start": 1381,
          "cds_end": null,
          "cds_length": 1866,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872308.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAGED2",
          "gene_hgnc_id": 16353,
          "hgvs_c": "c.1381C>T",
          "hgvs_p": "p.Arg461Cys",
          "transcript": "ENST00000872311.1",
          "protein_id": "ENSP00000542370.1",
          "transcript_support_level": null,
          "aa_start": 461,
          "aa_end": null,
          "aa_length": 621,
          "cds_start": 1381,
          "cds_end": null,
          "cds_length": 1866,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872311.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAGED2",
          "gene_hgnc_id": 16353,
          "hgvs_c": "c.1381C>T",
          "hgvs_p": "p.Arg461Cys",
          "transcript": "ENST00000919668.1",
          "protein_id": "ENSP00000589727.1",
          "transcript_support_level": null,
          "aa_start": 461,
          "aa_end": null,
          "aa_length": 621,
          "cds_start": 1381,
          "cds_end": null,
          "cds_length": 1866,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919668.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAGED2",
          "gene_hgnc_id": 16353,
          "hgvs_c": "c.1381C>T",
          "hgvs_p": "p.Arg461Cys",
          "transcript": "ENST00000944664.1",
          "protein_id": "ENSP00000614723.1",
          "transcript_support_level": null,
          "aa_start": 461,
          "aa_end": null,
          "aa_length": 621,
          "cds_start": 1381,
          "cds_end": null,
          "cds_length": 1866,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000944664.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAGED2",
          "gene_hgnc_id": 16353,
          "hgvs_c": "c.1336C>T",
          "hgvs_p": "p.Arg446Cys",
          "transcript": "ENST00000919658.1",
          "protein_id": "ENSP00000589717.1",
          "transcript_support_level": null,
          "aa_start": 446,
          "aa_end": null,
          "aa_length": 614,
          "cds_start": 1336,
          "cds_end": null,
          "cds_length": 1845,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919658.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAGED2",
          "gene_hgnc_id": 16353,
          "hgvs_c": "c.1336C>T",
          "hgvs_p": "p.Arg446Cys",
          "transcript": "NM_014599.6",
          "protein_id": "NP_055414.2",
          "transcript_support_level": null,
          "aa_start": 446,
          "aa_end": null,
          "aa_length": 606,
          "cds_start": 1336,
          "cds_end": null,
          "cds_length": 1821,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_014599.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAGED2",
          "gene_hgnc_id": 16353,
          "hgvs_c": "c.1336C>T",
          "hgvs_p": "p.Arg446Cys",
          "transcript": "NM_201222.3",
          "protein_id": "NP_957516.1",
          "transcript_support_level": null,
          "aa_start": 446,
          "aa_end": null,
          "aa_length": 606,
          "cds_start": 1336,
          "cds_end": null,
          "cds_length": 1821,
          "cdna_start": null,
          "cdna_end": null,
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          "transcript": "NR_002953.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "snoRNA",
          "feature": "NR_002953.1"
        }
      ],
      "gene_symbol": "MAGED2",
      "gene_hgnc_id": 16353,
      "dbsnp": "rs878854407",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": 0,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 0,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9793791770935059,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.404,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.9936,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.01,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 1.335,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 8,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PP3_Strong,PP5_Moderate",
      "acmg_by_gene": [
        {
          "score": 8,
          "benign_score": 0,
          "pathogenic_score": 8,
          "criteria": [
            "PM2",
            "PP3_Strong",
            "PP5_Moderate"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_014599.6",
          "gene_symbol": "MAGED2",
          "hgnc_id": 16353,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "XL,AR",
          "hgvs_c": "c.1336C>T",
          "hgvs_p": "p.Arg446Cys"
        },
        {
          "score": 8,
          "benign_score": 0,
          "pathogenic_score": 8,
          "criteria": [
            "PM2",
            "PP3_Strong",
            "PP5_Moderate"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NR_002953.1",
          "gene_symbol": "SNORA11",
          "hgnc_id": 32599,
          "effects": [
            "downstream_gene_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.*225C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Bartter disease type 5,not provided",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LP:1",
      "phenotype_combined": "Bartter disease type 5|not provided",
      "pathogenicity_classification_combined": "Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}