Reverse Complement DNA Sequence
Generate the reverse complement of a DNA sequence — essential for PCR primer design and genome analysis.
Reverse Complement Sequence:
The result will appear here.
This tool generates the reverse complement of a DNA sequence by first complementing each nucleotide (A↔T, C↔G) and then reversing the result.
Reverse complement sequences are commonly used in molecular biology, PCR primer design, sequence alignment, and genome analysis because they represent the sequence of the opposing strand read in the 5′→3′ direction.
Paste your DNA sequence below to instantly compute the reverse complement.
FAQ
What is a reverse complement DNA sequence?
A reverse complement is the DNA sequence obtained by reversing the original sequence and replacing each nucleotide with its complement (A↔T, C↔G). It represents the sequence of the opposite strand read in the 5′→3′ direction.
When do you need the reverse complement?
Reverse complements are used in primer design, sequence alignment, BLAST searches, and genome analysis. When designing a reverse primer for PCR, you need the reverse complement of your target region.
What is the difference between complement and reverse complement?
The complement keeps the same sequence order but swaps each base. The reverse complement additionally reverses the sequence, giving the correct 5′→3′ sequence of the opposing strand.
Can this tool handle long sequences?
Yes, the tool supports long DNA sequences used in research, primer design, and bioinformatics.