10-43119692-A-G

Variant names:

Variant summary

Our verdict is Benign. The variant received -16 ACMG points: 0P and 16B. BP4_StrongBP6_Very_StrongBS1

The NM_020975.6(RET):c.2554A>G(p.Ile852Val) variant causes a missense change. The variant allele was found at a frequency of 0.0000607 in 1,613,458 control chromosomes in the GnomAD database, with no homozygous occurrence. In-silico tool predicts a benign outcome for this variant. Variant has been reported in ClinVar as Likely benign (★★). Another variant affecting the same amino acid position, but resulting in a different missense (i.e. I852M) has been classified as Uncertain significance.

Frequency

Genomes: 𝑓 0.000039 ( 0 hom., cov: 34)

Exomes 𝑓: 0.000063 ( 0 hom. )

Consequence

RET
NM_020975.6 missense

Scores

Clinical Significance

Benign/Likely benign criteria provided, multiple submitters, no conflicts B:5

Conservation

PhyloP100: 3.78

Publications

1 publications found

Variant links:

Genes affected

RET (HGNC:9967): (ret proto-oncogene) This gene encodes a transmembrane receptor and member of the tyrosine protein kinase family of proteins. Binding of ligands such as GDNF (glial cell-line derived neurotrophic factor) and other related proteins to the encoded receptor stimulates receptor dimerization and activation of downstream signaling pathways that play a role in cell differentiation, growth, migration and survival. The encoded receptor is important in development of the nervous system, and the development of organs and tissues derived from the neural crest. This proto-oncogene can undergo oncogenic activation through both cytogenetic rearrangement and activating point mutations. Mutations in this gene are associated with Hirschsprung disease and central hypoventilation syndrome and have been identified in patients with renal agenesis. [provided by RefSeq, Sep 2017]

RET Gene-Disease associations (from GenCC):

familial medullary thyroid carcinoma
Inheritance: AD Classification: DEFINITIVE, SUPPORTIVE Submitted by: Orphanet, G2P
multiple endocrine neoplasia type 2A
Inheritance: AD Classification: DEFINITIVE, SUPPORTIVE Submitted by: Orphanet, ClinGen, G2P
multiple endocrine neoplasia type 2B
Inheritance: AD Classification: DEFINITIVE, STRONG, SUPPORTIVE Submitted by: G2P, Orphanet, Labcorp Genetics (formerly Invitae), ClinGen
pheochromocytoma
Inheritance: AD Classification: DEFINITIVE Submitted by: G2P
Hirschsprung disease, susceptibility to, 1
Inheritance: AD Classification: STRONG Submitted by: Labcorp Genetics (formerly Invitae)
Haddad syndrome
Inheritance: AD Classification: SUPPORTIVE Submitted by: Orphanet
Hirschsprung disease
Inheritance: AD Classification: SUPPORTIVE Submitted by: Orphanet
renal agenesis, unilateral
Inheritance: AD Classification: SUPPORTIVE Submitted by: Orphanet
bilateral renal agenesis
Inheritance: AR Classification: SUPPORTIVE Submitted by: Orphanet
renal agenesis
Inheritance: AR Classification: LIMITED Submitted by: G2P

RET links:

Genome browser will be placed here

ACMG classification

Classification was made for transcript

Our verdict: Benign. The variant received -16 ACMG points.

BP4

Computational evidence support a benign effect (MetaRNN=0.050564468).

BP6

Variant 10-43119692-A-G is Benign according to our data. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-43119692-A-G is described in CliVar as Benign/Likely_benign. Clinvar id is 241349.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars.

BS1

Variant frequency is greater than expected in population sas. GnomAd4 allele frequency = 0.0000394 (6/152228) while in subpopulation SAS AF = 0.00124 (6/4830). AF 95% confidence interval is 0.000541. There are 0 homozygotes in GnomAd4. There are 4 alleles in the male GnomAd4 subpopulation. Median coverage is 34. This position passed quality control check.

Transcripts

RefSeq

Gene	Transcript	HGVSc	HGVSp	Effect	Exon rank	MANE	Protein	UniProt
RET	NM_020975.6 link	c.2554A>G	p.Ile852Val	missense_variant	Exon 14 of 20	ENST00000355710.8	NP_066124.1	P07949-1A0A024R7T2

Ensembl

Gene	Transcript	HGVSc	HGVSp	Effect	Exon rank	TSL	MANE	Protein	Appris	UniProt
RET	ENST00000355710.8 link	c.2554A>G	p.Ile852Val	missense_variant	Exon 14 of 20	5	NM_020975.6	ENSP00000347942.3		P07949-1

Frequencies

GnomAD3 genomes

AF:

0.0000394

AC:

AN:

152110

Hom.:

Cov.:

show subpopulations

Gnomad AFR

AF:

0.00

Gnomad AMI

AF:

0.00

Gnomad AMR

AF:

0.00

Gnomad ASJ

AF:

0.00

Gnomad EAS

AF:

0.00

Gnomad SAS

AF:

0.00124

Gnomad FIN

AF:

0.00

Gnomad MID

AF:

0.00

Gnomad NFE

AF:

0.00

Gnomad OTH

AF:

0.00

GnomAD2 exomes

AF:

0.000171

AC:

AN:

250808

AF XY:

0.000243

show subpopulations

Gnomad AFR exome

AF:

0.0000616

Gnomad AMR exome

AF:

0.00

Gnomad ASJ exome

AF:

0.00

Gnomad EAS exome

AF:

0.00

Gnomad FIN exome

AF:

0.00

Gnomad NFE exome

AF:

0.00

Gnomad OTH exome

AF:

0.000653

GnomAD4 exome

AF:

0.0000630

AC:

AN:

1461230

Hom.:

Cov.:

AF XY:

0.0000825

AC XY:

AN XY:

726934

show subpopulations

African (AFR)

AF:

0.0000299

AC:

AN:

33478

American (AMR)

AF:

0.00

AC:

AN:

44718

Ashkenazi Jewish (ASJ)

AF:

0.00

AC:

AN:

26126

East Asian (EAS)

AF:

0.00

AC:

AN:

39696

South Asian (SAS)

AF:

0.000962

AC:

AN:

86258

European-Finnish (FIN)

AF:

0.00

AC:

AN:

52860

Middle Eastern (MID)

AF:

0.00

AC:

AN:

5766

European-Non Finnish (NFE)

AF:

0.00

AC:

AN:

1111950

Other (OTH)

AF:

0.000132

AC:

AN:

60378

Allele Balance Distribution

Red line indicates average allele balance

Average allele balance: 0.490

Heterozygous variant carriers

0.00

0.20

0.40

0.60

0.80

0.95

Allele balance

Age Distribution

Exome Het

Variant carriers

<30

30-35

35-40

40-45

45-50

50-55

55-60

60-65

65-70

70-75

75-80

>80

Age

GnomAD4 genome

AF:

0.0000394

AC:

AN:

152228

Hom.:

Cov.:

AF XY:

0.0000537

AC XY:

AN XY:

74424

show subpopulations

African (AFR)

AF:

0.00

AC:

AN:

41534

American (AMR)

AF:

0.00

AC:

AN:

15308

Ashkenazi Jewish (ASJ)

AF:

0.00

AC:

AN:

3468

East Asian (EAS)

AF:

0.00

AC:

AN:

5170

South Asian (SAS)

AF:

0.00124

AC:

AN:

4830

European-Finnish (FIN)

AF:

0.00

AC:

AN:

10612

Middle Eastern (MID)

AF:

0.00

AC:

AN:

294

European-Non Finnish (NFE)

AF:

0.00

AC:

AN:

67986

Other (OTH)

AF:

0.00

AC:

AN:

2114

Allele Balance Distribution

Red line indicates average allele balance

Average allele balance: 0.492

Heterozygous variant carriers

0.00

0.20

0.40

0.60

0.80

0.95

Allele balance

Age Distribution

Genome Het

Variant carriers

<30

30-35

35-40

40-45

45-50

50-55

55-60

60-65

65-70

70-75

75-80

>80

Age

Alfa

AF:

0.0000497

Hom.:

TwinsUK

AF:

0.00

AC:

ALSPAC

AF:

0.000259

AC:

ExAC

AF:

0.000214

AC:

Asia WGS

AF:

0.000577

AC:

AN:

3478

ClinVar

Significance: Benign/Likely benign

Submissions summary: Benign:5

Revision: criteria provided, multiple submitters, no conflicts

LINK: link

Submissions by phenotype

Multiple endocrine neoplasia type 2B

Benign:1

Jul 07, 2023

KCCC/NGS Laboratory, Kuwait Cancer Control Center

Significance:Likely benign

Review Status:criteria provided, single submitter

Collection Method:clinical testing

- -

Multiple endocrine neoplasia type 2A

Benign:1

Aug 13, 2024

Myriad Genetics, Inc.

Significance:Likely benign

Review Status:criteria provided, single submitter

Collection Method:clinical testing

This variant is considered likely benign. This variant has been observed at a population frequency that is significantly greater than expected given the associated disease prevalence and penetrance. -

Multiple endocrine neoplasia, type 2

Benign:1

Jan 29, 2025

Labcorp Genetics (formerly Invitae), Labcorp

Significance:Likely benign

Review Status:criteria provided, single submitter

Collection Method:clinical testing

- -

Hereditary cancer-predisposing syndrome

Benign:1

Sep 01, 2021

Ambry Genetics

Significance:Benign

Review Status:criteria provided, single submitter

Collection Method:clinical testing

This alteration is classified as benign based on a combination of the following: seen in unaffected individuals, population frequency, intact protein function, lack of segregation with disease, co-occurrence, RNA analysis, in silico models, amino acid conservation, lack of disease association in case-control studies, and/or the mechanism of disease or impacted region is inconsistent with a known cause of pathogenicity. -

RET-related disorder

Benign:1

May 16, 2024

PreventionGenetics, part of Exact Sciences

Significance:Likely benign

Review Status:no assertion criteria provided

Collection Method:clinical testing

This variant is classified as likely benign based on ACMG/AMP sequence variant interpretation guidelines (Richards et al. 2015 PMID: 25741868, with internal and published modifications). -

Computational scores

Source: dbNSFP v4.3

Name

Calibrated prediction

Score

Prediction

AlphaMissense

Benign

0.19

BayesDel_addAF

Benign

-0.27

BayesDel_noAF

Benign

-0.20

CADD

Benign

(source)

DANN

Uncertain

1.0

DEOGEN2

Uncertain

0.57

D;.

Eigen

Uncertain

0.34

Eigen_PC

Uncertain

0.40

FATHMM_MKL

Uncertain

0.92

LIST_S2

Uncertain

0.94

D;D

M_CAP

Benign

0.081

MetaRNN

Benign

0.051

T;T

MetaSVM

Uncertain

-0.13

MutationAssessor

Benign

0.75

N;N

PhyloP100

3.8

PrimateAI

Uncertain

0.70

PROVEAN

Benign

-0.90

N;N

REVEL

Uncertain

0.47

Sift

Benign

0.21

T;D

Sift4G

Benign

0.43

T;T

Polyphen

1.0

D;D

Vest4

0.29

MutPred

0.58

Gain of phosphorylation at T847 (P = 0.21);Gain of phosphorylation at T847 (P = 0.21);

MVP

0.57

MPC

0.31

ClinPred

0.060

GERP RS

5.4

Varity_R

0.57

gMVP

0.54

Mutation Taster

=50/50

(source)

Splicing

Name

Calibrated prediction

Score

Prediction

SpliceAI score (max)

0.010

Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Publications

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Variant summary

Frequency

Consequence

Scores

Clinical Significance

Conservation

Publications

ACMG classification

Transcripts

RefSeq

Ensembl

Frequencies

Age Distribution

Age Distribution

ClinVar

Submissions by phenotype

Computational scores

Splicing

Publications

Other links and lift over