10-86706708-C-T

Variant names:

Variant summary

Our verdict is Benign. The variant received -21 ACMG points: 0P and 21B. BP4_StrongBP6_Very_StrongBP7BA1

The NM_007078.3(LDB3):c.1074C>T(p.Ala358Ala) variant causes a synonymous change involving the alteration of a non-conserved nucleotide. The variant allele was found at a frequency of 0.0377 in 1,611,890 control chromosomes in the GnomAD database, including 1,352 homozygotes. In-silico tool predicts a benign outcome for this variant. Variant has been reported in ClinVar as Benign (★★). Synonymous variant affecting the same amino acid position (i.e. A358A) has been classified as Uncertain significance.

Frequency

Genomes: 𝑓 0.027 ( 88 hom., cov: 33)

Exomes 𝑓: 0.039 ( 1264 hom. )

Consequence

LDB3
NM_007078.3 synonymous

Scores

Clinical Significance

Benign criteria provided, multiple submitters, no conflicts U:1B:14

Conservation

PhyloP100: 0.0710

Publications

5 publications found

Variant links:

Genes affected

LDB3 (HGNC:15710): (LIM domain binding 3) This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. The LIM domain is a cysteine-rich motif defined by 50-60 amino acids containing two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Mutations in this gene have been associated with myofibrillar myopathy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been identified; all isoforms have N-terminal PDZ domains while only longer isoforms (1, 2 and 5) have C-terminal LIM domains. [provided by RefSeq, Jan 2010]

LDB3 Gene-Disease associations (from GenCC):

myofibrillar myopathy 4
Inheritance: AD Classification: STRONG, MODERATE, LIMITED Submitted by: G2P, Labcorp Genetics (formerly Invitae), Ambry Genetics
familial isolated dilated cardiomyopathy
Inheritance: AD Classification: SUPPORTIVE Submitted by: Orphanet
dilated cardiomyopathy
Inheritance: AD Classification: LIMITED Submitted by: ClinGen
familial dilated cardiomyopathy
Inheritance: AR Classification: LIMITED Submitted by: Ambry Genetics
arrhythmogenic right ventricular cardiomyopathy
Inheritance: AD Classification: NO_KNOWN Submitted by: ClinGen

LDB3 links:

Genome browser will be placed here

ACMG classification

Classification was made for transcript

Our verdict: Benign. The variant received -21 ACMG points.

BP4

Computational evidence support a benign effect (BayesDel_noAF=-0.82).

BP6

Variant 10-86706708-C-T is Benign according to our data. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr10-86706708-C-T is described in CliVar as Benign. Clinvar id is 36443.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars.

BP7

Synonymous conserved (PhyloP=0.071 with no splicing effect.

BA1

GnomAdExome4 highest subpopulation (SAS) allele frequency at 95% confidence interval = 0.0569 is higher than 0.05.

Transcripts

RefSeq

Gene	Transcript	HGVSc	HGVSp	Effect	Exon rank	MANE	Protein	UniProt
LDB3	NM_007078.3 link	c.1074C>T	p.Ala358Ala	synonymous_variant	Exon 8 of 14	ENST00000361373.9	NP_009009.1	O75112-1

Ensembl

Gene	Transcript	HGVSc	HGVSp	Effect	Exon rank	TSL	MANE	Protein	Appris	UniProt
LDB3	ENST00000361373.9 link	c.1074C>T	p.Ala358Ala	synonymous_variant	Exon 8 of 14	1	NM_007078.3	ENSP00000355296.3		O75112-1

Frequencies

GnomAD3 genomes

AF:

0.0274

AC:

4167

AN:

152166

Hom.:

Cov.:

show subpopulations

Gnomad AFR

AF:

0.00654

Gnomad AMI

AF:

0.0658

Gnomad AMR

AF:

0.0202

Gnomad ASJ

AF:

0.00893

Gnomad EAS

AF:

0.000385

Gnomad SAS

AF:

0.0510

Gnomad FIN

AF:

0.0274

Gnomad MID

AF:

0.0285

Gnomad NFE

AF:

0.0427

Gnomad OTH

AF:

0.0201

GnomAD2 exomes

AF:

0.0327

AC:

7970

AN:

244014

AF XY:

0.0355

show subpopulations

Gnomad AFR exome

AF:

0.00481

Gnomad AMR exome

AF:

0.0136

Gnomad ASJ exome

AF:

0.0117

Gnomad EAS exome

AF:

0.000439

Gnomad FIN exome

AF:

0.0278

Gnomad NFE exome

AF:

0.0443

Gnomad OTH exome

AF:

0.0359

GnomAD4 exome

AF:

0.0388

AC:

56602

AN:

1459606

Hom.:

1264

Cov.:

AF XY:

0.0397

AC XY:

28843

AN XY:

726062

show subpopulations

African (AFR)

AF:

0.00574

AC:

192

AN:

33442

American (AMR)

AF:

0.0129

AC:

577

AN:

44568

Ashkenazi Jewish (ASJ)

AF:

0.0109

AC:

284

AN:

26058

East Asian (EAS)

AF:

0.000227

AC:

AN:

39660

South Asian (SAS)

AF:

0.0582

AC:

5018

AN:

86148

European-Finnish (FIN)

AF:

0.0310

AC:

1632

AN:

52608

Middle Eastern (MID)

AF:

0.0323

AC:

178

AN:

5508

European-Non Finnish (NFE)

AF:

0.0420

AC:

46725

AN:

1111340

Other (OTH)

AF:

0.0330

AC:

1987

AN:

60274

Allele Balance Distribution

Red line indicates average allele balance

Average allele balance: 0.481

Heterozygous variant carriers

3013

6027

9040

12054

15067

0.00

0.20

0.40

0.60

0.80

0.95

Allele balance

Age Distribution

Exome Het

Exome Hom

Variant carriers

1674

3348

5022

6696

8370

<30

30-35

35-40

40-45

45-50

50-55

55-60

60-65

65-70

70-75

75-80

>80

Age

GnomAD4 genome

AF:

0.0273

AC:

4162

AN:

152284

Hom.:

Cov.:

AF XY:

0.0274

AC XY:

2037

AN XY:

74468

show subpopulations

African (AFR)

AF:

0.00652

AC:

271

AN:

41554

American (AMR)

AF:

0.0201

AC:

308

AN:

15304

Ashkenazi Jewish (ASJ)

AF:

0.00893

AC:

AN:

3470

East Asian (EAS)

AF:

0.000386

AC:

AN:

5184

South Asian (SAS)

AF:

0.0506

AC:

244

AN:

4818

European-Finnish (FIN)

AF:

0.0274

AC:

291

AN:

10622

Middle Eastern (MID)

AF:

0.0272

AC:

AN:

294

European-Non Finnish (NFE)

AF:

0.0427

AC:

2906

AN:

68012

Other (OTH)

AF:

0.0194

AC:

AN:

2114

Allele Balance Distribution

Red line indicates average allele balance

Average allele balance: 0.501

Heterozygous variant carriers

216

433

649

866

1082

0.00

0.20

0.40

0.60

0.80

0.95

Allele balance

Age Distribution

Genome Het

Genome Hom

Variant carriers

104

156

208

260

<30

30-35

35-40

40-45

45-50

50-55

55-60

60-65

65-70

70-75

75-80

>80

Age

Alfa

AF:

0.0313

Hom.:

Bravo

AF:

0.0235

Asia WGS

AF:

0.0180

AC:

AN:

3478

ClinVar

Significance: Benign

Submissions summary: Uncertain:1Benign:14

Revision: criteria provided, multiple submitters, no conflicts

LINK: link

Submissions by phenotype

not specified

Benign:7

Jun 24, 2008

Laboratory for Molecular Medicine, Mass General Brigham Personalized Medicine

Significance:Benign

Review Status:criteria provided, single submitter

Collection Method:clinical testing

- -

PreventionGenetics, part of Exact Sciences

Significance:Benign

Review Status:criteria provided, single submitter

Collection Method:clinical testing

- -

Genome Diagnostics Laboratory, University Medical Center Utrecht

Significance:Benign

Review Status:no assertion criteria provided

Collection Method:clinical testing

- -

Clinical Genetics, Academic Medical Center

Significance:Benign

Review Status:no assertion criteria provided

Collection Method:clinical testing

- -

Clinical Genetics DNA and cytogenetics Diagnostics Lab, Erasmus MC, Erasmus Medical Center

Significance:Benign

Review Status:no assertion criteria provided

Collection Method:clinical testing

- -

Oct 29, 2024

Athena Diagnostics

Significance:Benign

Review Status:criteria provided, single submitter

Collection Method:clinical testing

- -

Joint Genome Diagnostic Labs from Nijmegen and Maastricht, Radboudumc and MUMC+

Significance:Benign

Review Status:no assertion criteria provided

Collection Method:clinical testing

- -

not provided

Benign:4

Diagnostic Laboratory, Department of Genetics, University Medical Center Groningen

Significance:Likely benign

Review Status:no assertion criteria provided

Collection Method:clinical testing

- -

Breakthrough Genomics, Breakthrough Genomics

Significance:Benign

Review Status:criteria provided, single submitter

Collection Method:not provided

- -

Mar 03, 2015

GeneDx

Significance:Benign

Review Status:criteria provided, single submitter

Collection Method:clinical testing

- -

Laboratory of Diagnostic Genome Analysis, Leiden University Medical Center (LUMC)

Significance:Likely benign

Review Status:no assertion criteria provided

Collection Method:clinical testing

- -

Dilated cardiomyopathy 1C

Uncertain:1

Aug 21, 2014

Cytogenetics- Mohapatra Lab, Banaras Hindu University

Significance:Uncertain significance

Review Status:no assertion criteria provided

Collection Method:case-control

- -

Cardiomyopathy

Benign:1

Aug 18, 2011

Women's Health and Genetics/Laboratory Corporation of America, LabCorp

Significance:Benign

Review Status:criteria provided, single submitter

Collection Method:curation

- -

Myofibrillar myopathy 4

Benign:1

Feb 03, 2025

Labcorp Genetics (formerly Invitae), Labcorp

Significance:Benign

Review Status:criteria provided, single submitter

Collection Method:clinical testing

- -

Cardiovascular phenotype

Benign:1

Jul 15, 2015

Ambry Genetics

Significance:Benign

Review Status:criteria provided, single submitter

Collection Method:clinical testing

This alteration is classified as benign based on a combination of the following: seen in unaffected individuals, population frequency, intact protein function, lack of segregation with disease, co-occurrence, RNA analysis, in silico models, amino acid conservation, lack of disease association in case-control studies, and/or the mechanism of disease or impacted region is inconsistent with a known cause of pathogenicity. -

Computational scores

Source: dbNSFP v4.3

Name

Calibrated prediction

Score

Prediction

BayesDel_noAF

Benign

-0.82

CADD

Benign

4.9

(source)

DANN

Benign

0.47

PhyloP100

0.071

Mutation Taster

=100/0

polymorphism (auto)

(source)

Splicing

Name

Calibrated prediction

Score

Prediction

SpliceAI score (max)

0.070

Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Publications

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Variant summary

Frequency

Consequence

Scores

Clinical Significance

Conservation

Publications

ACMG classification

Transcripts

RefSeq

Ensembl

Frequencies

Age Distribution

Age Distribution

ClinVar

Submissions by phenotype

Computational scores

Splicing

Publications

Other links and lift over