18-69698432-A-G

Variant names:

• chr18-69698432-A-G
• rs4426448
• NM_152721.6:c.438A>G

Variant summary

Our verdict is Benign. The variant received -13 ACMG points: 0P and 13B. BP4_Strong BP7 BA1

The NM_152721.6(DOK6):​c.438A>G​(p.Thr146Thr) variant causes a synonymous change involving the alteration of a non-conserved nucleotide. The variant allele was found at a frequency of 0.465 in 1,612,720 control chromosomes in the GnomAD database, including 176,726 homozygotes. In-silico tool predicts a benign outcome for this variant. No clinical diagnostic laboratories have submitted clinical-significance assessments for this variant to ClinVar.

• Frequency:
  • Genomes: 𝑓 0.46 (16419 hom., cov: 32)
  • Exomes 𝑓: 0.47 (160307 hom.)
• Consequence: DOK6 NM_152721.6 synonymous
• Clinical Significance: Not reported in ClinVar
• Conservation: PhyloP100: -0.795
• Publications: 17 publications found

Genes affected

DOK6 (HGNC:28301): (docking protein 6) DOK6 is a member of the DOK (see DOK1; MIM 602919) family of intracellular adaptors that play a role in the RET (MIM 164761) signaling cascade (Crowder et al., 2004 [PubMed 15286081]).[supplied by OMIM, Mar 2008]

ACMG classification

Our verdict: Benign. The variant received -13 ACMG points.

• BP4: Computational evidence support a benign effect (BayesDel_noAF=-0.84).
• BP7: Synonymous conserved (PhyloP=-0.795 with no splicing effect.
• BA1: GnomAd4 highest subpopulation (EAS) allele frequency at 95% confidence interval = 0.71 is higher than 0.05.

Variant Effect in Transcripts

ACMG analysis was done for transcript: NM_152721.6. You can select a different transcript below to see updated ACMG assignments.

RefSeq Transcripts

Sel.	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	DOK6	NM_152721.6	MANE Select	c.438A>G	p.Thr146Thr	synonymous	Exon 5 of 8	NP_689934.2

Ensembl Transcripts

Sel.	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	DOK6	ENST00000382713.10	TSL:1 MANE Select	c.438A>G	p.Thr146Thr	synonymous	Exon 5 of 8	ENSP00000372160.5	Q6PKX4
	DOK6	ENST00000893242.1		c.318A>G	p.Thr106Thr	synonymous	Exon 4 of 7	ENSP00000563301.1
	DOK6	ENST00000582992.1	TSL:3	c.147A>G	p.Thr49Thr	synonymous	Exon 2 of 3	ENSP00000462984.1	J3KTH4

Frequencies

GnomAD3 genomes AF: 0.460 AC: 69844 AN: 151964 Hom.: 16415 Cov.: 32

Gnomad AFR AF: 0.444

Gnomad AMI AF: 0.520

Gnomad AMR AF: 0.396

Gnomad ASJ AF: 0.380

Gnomad EAS AF: 0.729

Gnomad SAS AF: 0.432

Gnomad FIN AF: 0.502

Gnomad MID AF: 0.389

Gnomad NFE AF: 0.462

Gnomad OTH AF: 0.452

GnomAD2 exomes AF: 0.464 AC: 116000 AN: 250210 AF XY: 0.463

Gnomad AFR exome AF: 0.435

Gnomad AMR exome AF: 0.369

Gnomad ASJ exome AF: 0.375

Gnomad EAS exome AF: 0.754

Gnomad FIN exome AF: 0.499

Gnomad NFE exome AF: 0.462

Gnomad OTH exome AF: 0.458

GnomAD4 exome AF: 0.466 AC: 680077 AN: 1460638 Hom.: 160307 Cov.: 42 AF XY: 0.464 AC XY: 337254 AN XY: 726648

African (AFR) AF: 0.432 AC: 14458 AN: 33438

American (AMR) AF: 0.378 AC: 16879 AN: 44640

Ashkenazi Jewish (ASJ) AF: 0.381 AC: 9926 AN: 26060

East Asian (EAS) AF: 0.672 AC: 26658 AN: 39668

South Asian (SAS) AF: 0.420 AC: 36151 AN: 86128

European-Finnish (FIN) AF: 0.495 AC: 26378 AN: 53296

Middle Eastern (MID) AF: 0.435 AC: 2508 AN: 5766

European-Non Finnish (NFE) AF: 0.467 AC: 518996 AN: 1111276

Other (OTH) AF: 0.466 AC: 28123 AN: 60366

GnomAD4 genome AF: 0.459 AC: 69878 AN: 152082 Hom.: 16419 Cov.: 32 AF XY: 0.459 AC XY: 34130 AN XY: 74342

African (AFR) AF: 0.444 AC: 18400 AN: 41486

American (AMR) AF: 0.396 AC: 6055 AN: 15284

Ashkenazi Jewish (ASJ) AF: 0.380 AC: 1318 AN: 3472

East Asian (EAS) AF: 0.729 AC: 3765 AN: 5162

South Asian (SAS) AF: 0.432 AC: 2086 AN: 4824

European-Finnish (FIN) AF: 0.502 AC: 5310 AN: 10570

Middle Eastern (MID) AF: 0.388 AC: 114 AN: 294

European-Non Finnish (NFE) AF: 0.462 AC: 31411 AN: 67970

Other (OTH) AF: 0.448 AC: 945 AN: 2108

Alfa AF: 0.455 Hom.: 24233

Bravo AF: 0.453

Asia WGS AF: 0.538 AC: 1873 AN: 3478

EpiCase AF: 0.454

EpiControl AF: 0.460

ClinVar

Not reported in ClinVar

Computational scores

Source: dbNSFP v4.9

Name	Calibrated prediction	Score	Prediction
BayesDel_noAF	Benign	-0.84
CADD	Benign	5.3
DANN	Benign	0.51
PhyloP100		-0.80
RBP_binding_hub_radar		0.0
RBP_regulation_power_radar		1.7
Mutation Taster		=98/2	polymorphism (auto)

Splicing

Name	Calibrated prediction	Score	Prediction
SpliceAI score (max)		0.0		Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Other links and lift over

dbSNP: rs4426448; hg19: chr18-67365668; COSMIC: COSV108228293;