22-33006858-C-T

Variant names:

• chr22-33006858-C-T
• rs133945
• NM_003490.4:c.-162-34G>A

Variant summary

Our verdict is Benign. The variant received -12 ACMG points: 0P and 12B. BP4_Strong BA1

The NM_003490.4(SYN3):​c.-162-34G>A variant causes a intron change involving the alteration of a non-conserved nucleotide. The variant allele was found at a frequency of 0.458 in 511,090 control chromosomes in the GnomAD database, including 54,868 homozygotes. In-silico tool predicts a benign outcome for this variant. No clinical diagnostic laboratories have submitted clinical-significance assessments for this variant to ClinVar.

• Frequency:
  • Genomes: 𝑓 0.45 (15422 hom., cov: 32)
  • Exomes 𝑓: 0.46 (39446 hom.)
• Consequence: SYN3 NM_003490.4 intron
• Clinical Significance: Not reported in ClinVar
• Conservation: PhyloP100: -4.91
• Publications: 21 publications found

Genes affected

SYN3 (HGNC:11496): (synapsin III) This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. The protein encoded by this gene shares the synapsin family domain model, with domains A, C, and E exhibiting the highest degree of conservation. The protein contains a unique domain J, located between domains C and E. Based on this gene's localization to 22q12.3, a possible schizophrenia susceptibility locus, and the established neurobiological roles of the synapsins, this family member may represent a candidate gene for schizophrenia. The TIMP3 gene is located within an intron of this gene and is transcribed in the opposite direction. Alternative splicing of this gene results in multiple splice variants that encode different isoforms. [provided by RefSeq, Oct 2008]

ACMG classification

Our verdict: Benign. The variant received -12 ACMG points.

• BP4: Computational evidence support a benign effect (BayesDel_noAF=-0.8).
• BA1: GnomAd4 highest subpopulation (NFE) allele frequency at 95% confidence interval = 0.483 is higher than 0.05.

Variant Effect in Transcripts

ACMG analysis was done for transcript: NM_003490.4. You can select a different transcript below to see updated ACMG assignments.

RefSeq Transcripts

Selected	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	SYN3	NM_003490.4	MANE Select	c.-162-34G>A		intron	N/A	NP_003481.3
	SYN3	NM_001369907.1		c.-159-37G>A		intron	N/A	NP_001356836.1
	SYN3	NM_001369908.1		c.-162-34G>A		intron	N/A	NP_001356837.1

Ensembl Transcripts

Selected	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	SYN3	ENST00000358763.7	TSL:5 MANE Select	c.-162-34G>A		intron	N/A	ENSP00000351614.2
	SYN3	ENST00000441821.5	TSL:1	c.-162-34G>A		intron	N/A	ENSP00000395794.1
	SYN3	ENST00000412575.1	TSL:5	c.-162-34G>A		intron	N/A	ENSP00000388582.1

Frequencies

GnomAD3 genomes AF: 0.446 AC: 67775 AN: 151840 Hom.: 15421 Cov.: 32

Gnomad AFR AF: 0.386

Gnomad AMI AF: 0.548

Gnomad AMR AF: 0.417

Gnomad ASJ AF: 0.520

Gnomad EAS AF: 0.326

Gnomad SAS AF: 0.432

Gnomad FIN AF: 0.481

Gnomad MID AF: 0.545

Gnomad NFE AF: 0.487

Gnomad OTH AF: 0.487

GnomAD4 exome AF: 0.463 AC: 166101 AN: 359132 Hom.: 39446 Cov.: 4 AF XY: 0.464 AC XY: 85104 AN XY: 183586

African (AFR) AF: 0.387 AC: 4491 AN: 11608

American (AMR) AF: 0.364 AC: 5418 AN: 14886

Ashkenazi Jewish (ASJ) AF: 0.500 AC: 5889 AN: 11768

East Asian (EAS) AF: 0.317 AC: 9161 AN: 28882

South Asian (SAS) AF: 0.441 AC: 9010 AN: 20434

European-Finnish (FIN) AF: 0.466 AC: 11631 AN: 24954

Middle Eastern (MID) AF: 0.532 AC: 910 AN: 1710

European-Non Finnish (NFE) AF: 0.490 AC: 109238 AN: 222970

Other (OTH) AF: 0.472 AC: 10353 AN: 21920

GnomAD4 genome AF: 0.446 AC: 67791 AN: 151958 Hom.: 15422 Cov.: 32 AF XY: 0.446 AC XY: 33156 AN XY: 74278

African (AFR) AF: 0.386 AC: 15994 AN: 41426

American (AMR) AF: 0.416 AC: 6364 AN: 15286

Ashkenazi Jewish (ASJ) AF: 0.520 AC: 1805 AN: 3470

East Asian (EAS) AF: 0.327 AC: 1685 AN: 5156

South Asian (SAS) AF: 0.433 AC: 2084 AN: 4818

European-Finnish (FIN) AF: 0.481 AC: 5070 AN: 10532

Middle Eastern (MID) AF: 0.545 AC: 159 AN: 292

European-Non Finnish (NFE) AF: 0.487 AC: 33114 AN: 67958

Other (OTH) AF: 0.482 AC: 1017 AN: 2110

Alfa AF: 0.474 Hom.: 38096

Bravo AF: 0.438

Asia WGS AF: 0.358 AC: 1247 AN: 3478

ClinVar

Not reported in ClinVar

Computational scores

Source: dbNSFP v4.9

Name	Calibrated prediction	Score	Prediction
BayesDel_noAF	Benign	-0.80
CADD	Benign	0.018
DANN	Benign	0.71
PhyloP100		-4.9
Mutation Taster		=100/0	polymorphism (auto)

Splicing

Name	Calibrated prediction	Score	Prediction
SpliceAI score (max)		0.0		Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Other links and lift over

dbSNP: rs133945; hg19: chr22-33402843;