4-154490731-G-A

Variant names:

• chr4-154490731-G-A
• rs17373860
• NM_001358235.2:c.625C>T

Variant summary

Our verdict is Benign. The variant received -13 ACMG points: 0P and 13B. BP4_Strong BP6 BA1

The NM_001358235.2(DCHS2):​c.625C>T​(p.Pro209Ser) variant causes a missense change involving the alteration of a non-conserved nucleotide. The variant allele was found at a frequency of 0.0692 in 1,551,632 control chromosomes in the GnomAD database, including 4,412 homozygotes. In-silico tool predicts a benign outcome for this variant. 13/17 in silico tools predict a benign outcome for this variant. Variant has been reported in ClinVar as Benign (no stars).

• Frequency:
  • Genomes: 𝑓 0.058 (407 hom., cov: 33)
  • Exomes 𝑓: 0.070 (4005 hom.)
• Consequence: DCHS2 NM_001358235.2 missense
• Clinical Significance: Benign no assertion criteria provided B:1
• Conservation: PhyloP100: -0.800
• Publications: 6 publications found

Genes affected

DCHS2 (HGNC:23111): (dachsous cadherin-related 2) This gene encodes a large protein that contains many cadherin domains and likely functions in cell adhesion. Genome-wide association studies suggest that this gene may be important in Alzheimer's disease, compressive strength index, and appendicular lean mass. [provided by RefSeq, May 2017]

ACMG classification

Our verdict: Benign. The variant received -13 ACMG points.

• BP4: Computational evidence support a benign effect (MetaRNN=0.0014812648).
• BP6: Variant 4-154490731-G-A is Benign according to our data. Variant chr4-154490731-G-A is described in ClinVar as Benign. ClinVar VariationId is 3059143.Status of the report is no_assertion_criteria_provided, 0 stars.
• BA1: GnomAd4 highest subpopulation (NFE) allele frequency at 95% confidence interval = 0.0747 is higher than 0.05.

Variant Effect in Transcripts

ACMG analysis was done for transcript: NM_001358235.2. You can select a different transcript below to see updated ACMG assignments.

RefSeq Transcripts

Selected	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	DCHS2	NM_001358235.2	MANE Select	c.625C>T	p.Pro209Ser	missense	Exon 1 of 20	NP_001345164.1
	DCHS2	NM_001142552.2		c.625C>T	p.Pro209Ser	missense	Exon 1 of 8	NP_001136024.1

Ensembl Transcripts

Selected	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	DCHS2	ENST00000357232.10	TSL:1 MANE Select	c.625C>T	p.Pro209Ser	missense	Exon 1 of 20	ENSP00000349768.5
	DCHS2	ENST00000339452.2	TSL:1	c.625C>T	p.Pro209Ser	missense	Exon 1 of 8	ENSP00000345062.1
	DCHS2	ENST00000456341.2	TSL:1	n.604C>T		non_coding_transcript_exon	Exon 1 of 2

Frequencies

GnomAD3 genomes AF: 0.0581 AC: 8836 AN: 152212 Hom.: 407 Cov.: 33

Gnomad AFR AF: 0.0114

Gnomad AMI AF: 0.124

Gnomad AMR AF: 0.0715

Gnomad ASJ AF: 0.0360

Gnomad EAS AF: 0.00154

Gnomad SAS AF: 0.0283

Gnomad FIN AF: 0.149

Gnomad MID AF: 0.0127

Gnomad NFE AF: 0.0764

Gnomad OTH AF: 0.0483

GnomAD2 exomes AF: 0.0690 AC: 10577 AN: 153180 AF XY: 0.0658

Gnomad AFR exome AF: 0.0119

Gnomad AMR exome AF: 0.0996

Gnomad ASJ exome AF: 0.0359

Gnomad EAS exome AF: 0.000276

Gnomad FIN exome AF: 0.157

Gnomad NFE exome AF: 0.0721

Gnomad OTH exome AF: 0.0593

GnomAD4 exome AF: 0.0704 AC: 98531 AN: 1399302 Hom.: 4005 Cov.: 92 AF XY: 0.0692 AC XY: 47769 AN XY: 690170

African (AFR) AF: 0.0101 AC: 319 AN: 31592

American (AMR) AF: 0.0981 AC: 3501 AN: 35704

Ashkenazi Jewish (ASJ) AF: 0.0385 AC: 969 AN: 25176

East Asian (EAS) AF: 0.000532 AC: 19 AN: 35732

South Asian (SAS) AF: 0.0345 AC: 2736 AN: 79236

European-Finnish (FIN) AF: 0.150 AC: 7380 AN: 49262

Middle Eastern (MID) AF: 0.0174 AC: 99 AN: 5698

European-Non Finnish (NFE) AF: 0.0743 AC: 80143 AN: 1078908

Other (OTH) AF: 0.0580 AC: 3365 AN: 57994

GnomAD4 genome AF: 0.0581 AC: 8849 AN: 152330 Hom.: 407 Cov.: 33 AF XY: 0.0598 AC XY: 4452 AN XY: 74486

African (AFR) AF: 0.0114 AC: 473 AN: 41588

American (AMR) AF: 0.0718 AC: 1100 AN: 15310

Ashkenazi Jewish (ASJ) AF: 0.0360 AC: 125 AN: 3470

East Asian (EAS) AF: 0.00155 AC: 8 AN: 5174

South Asian (SAS) AF: 0.0290 AC: 140 AN: 4830

European-Finnish (FIN) AF: 0.149 AC: 1586 AN: 10624

Middle Eastern (MID) AF: 0.0136 AC: 4 AN: 294

European-Non Finnish (NFE) AF: 0.0764 AC: 5199 AN: 68016

Other (OTH) AF: 0.0478 AC: 101 AN: 2112

Alfa AF: 0.0655 Hom.: 764

Bravo AF: 0.0504

TwinsUK AF: 0.0717 AC: 266

ALSPAC AF: 0.0651 AC: 251

ESP6500AA AF: 0.0101 AC: 14

ESP6500EA AF: 0.0817 AC: 260

ExAC AF: 0.0454 AC: 1183

Asia WGS AF: 0.0200 AC: 71 AN: 3478

ClinVar

Significance: Benign

Submissions summary: Benign:1

Revision: no assertion criteria provided

Submissions by phenotype

DCHS2-related disorder Benign:1

May 01, 2019

PreventionGenetics, part of Exact Sciences

Significance: Benign

Review Status: no assertion criteria provided

Collection Method: clinical testing

This variant is classified as benign based on ACMG/AMP sequence variant interpretation guidelines (Richards et al. 2015 PMID: 25741868, with internal and published modifications).

Computational scores

Source: dbNSFP v4.9

Name	Calibrated prediction	Score	Prediction
AlphaMissense	Benign	0.085
BayesDel_addAF	Benign	-0.71	T
BayesDel_noAF	Benign	-0.72
CADD	Benign	1.6
DANN	Benign	0.91
Eigen	Benign	-1.1
Eigen_PC	Benign	-1.1
FATHMM_MKL	Benign	0.25	N
MetaRNN	Benign	0.0015	T
MetaSVM	Benign	-1.1	T
PhyloP100		-0.80
PROVEAN	Benign	0.18	N
REVEL	Benign	0.034
Sift	Benign	0.64	T
Sift4G	Benign	0.32	T
Vest4		0.024
ClinPred		0.0059	T
GERP RS		-0.34
gMVP		0.49
Mutation Taster		=100/0	polymorphism (auto)

Splicing

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Other links and lift over

dbSNP: rs17373860; hg19: chr4-155411883; COSMIC: COSV59728481;