4-25312898-C-T
Variant summary
Our verdict is Benign. Variant got -14 ACMG points: 0P and 14B. BP4_StrongBP6_ModerateBS1BS2
The NM_024936.3(ZCCHC4):c.89C>T(p.Pro30Leu) variant causes a missense change involving the alteration of a non-conserved nucleotide. The variant allele was found at a frequency of 0.00196 in 1,612,906 control chromosomes in the GnomAD database, including 59 homozygotes. In-silico tool predicts a benign outcome for this variant. 13/20 in silico tools predict a benign outcome for this variant. Variant has been reported in ClinVar as Benign (★).
Frequency
Genomes: 𝑓 0.011 ( 31 hom., cov: 32)
Exomes 𝑓: 0.0011 ( 28 hom. )
Consequence
ZCCHC4
NM_024936.3 missense
NM_024936.3 missense
Scores
1
17
Clinical Significance
Conservation
PhyloP100: 0.147
Genes affected
ZCCHC4 (HGNC:22917): (zinc finger CCHC-type containing 4) Enables S-adenosyl-L-methionine binding activity; rRNA (adenine-N6-)-methyltransferase activity; and zinc ion binding activity. Involved in positive regulation of translation and rRNA methylation. Located in cytoplasm and nucleolus. [provided by Alliance of Genome Resources, Apr 2022]
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ACMG classification
Classification made for transcript
Verdict is Benign. Variant got -14 ACMG points.
BP4
?
Computational evidence support a benign effect (MetaRNN=0.0030532181).
BP6
?
Variant 4-25312898-C-T is Benign according to our data. Variant chr4-25312898-C-T is described in ClinVar as [Benign]. Clinvar id is 791974.Status of the report is criteria_provided_single_submitter, 1 stars.
BS1
?
Variant frequency is greater than expected in population afr. gnomad4 allele frequency = 0.0106 (1618/152230) while in subpopulation AFR AF= 0.0374 (1555/41534). AF 95% confidence interval is 0.0359. There are 31 homozygotes in gnomad4. There are 747 alleles in male gnomad4 subpopulation. Median coverage is 32. This position pass quality control queck.
BS2
?
High Homozygotes in GnomAd at 31 AR gene
Transcripts
RefSeq
Gene | Transcript | HGVSc | HGVSp | Effect | #exon/exons | MANE | UniProt |
---|---|---|---|---|---|---|---|
ZCCHC4 | NM_024936.3 | c.89C>T | p.Pro30Leu | missense_variant | 1/13 | ENST00000302874.9 |
Ensembl
Gene | Transcript | HGVSc | HGVSp | Effect | #exon/exons | TSL | MANE | Appris | UniProt |
---|---|---|---|---|---|---|---|---|---|
ZCCHC4 | ENST00000302874.9 | c.89C>T | p.Pro30Leu | missense_variant | 1/13 | 1 | NM_024936.3 | P1 | |
ZCCHC4 | ENST00000505451.5 | n.114C>T | non_coding_transcript_exon_variant | 1/9 | 1 | ||||
ZCCHC4 | ENST00000507760.5 | c.89C>T | p.Pro30Leu | missense_variant, NMD_transcript_variant | 1/9 | 1 |
Frequencies
GnomAD3 genomes ? AF: 0.0106 AC: 1615AN: 152112Hom.: 31 Cov.: 32
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GnomAD3 exomes AF: 0.00268 AC: 669AN: 249262Hom.: 14 AF XY: 0.00191 AC XY: 258AN XY: 135282
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GnomAD4 exome AF: 0.00106 AC: 1542AN: 1460676Hom.: 28 Cov.: 31 AF XY: 0.000881 AC XY: 640AN XY: 726622
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GnomAD4 genome ? AF: 0.0106 AC: 1618AN: 152230Hom.: 31 Cov.: 32 AF XY: 0.0100 AC XY: 747AN XY: 74424
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ESP6500AA
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422
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ClinVar
Significance: Benign
Submissions summary: Benign:1
Revision: criteria provided, single submitter
LINK: link
Submissions by phenotype
not provided Benign:1
Benign, criteria provided, single submitter | clinical testing | Invitae | Dec 31, 2019 | - - |
Computational scores
Source:
Name
Calibrated prediction
Score
Prediction
AlphaMissense
Benign
BayesDel_addAF
Benign
T
BayesDel_noAF
Benign
Cadd
Benign
Dann
Uncertain
DEOGEN2
Benign
T;T
Eigen
Benign
Eigen_PC
Benign
FATHMM_MKL
Benign
N
LIST_S2
Benign
T;T
MetaRNN
Benign
T;T
MetaSVM
Benign
T
MutationAssessor
Benign
N;.
MutationTaster
Benign
N
PrimateAI
Benign
T
PROVEAN
Benign
N;.
REVEL
Benign
Sift
Benign
T;.
Sift4G
Benign
T;T
Polyphen
B;.
Vest4
MVP
MPC
ClinPred
T
GERP RS
Varity_R
gMVP
Splicing
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SpliceAI score (max)
Details are displayed if max score is > 0.2
Find out detailed SpliceAI scores and Pangolin per-transcript scores at