7-55181316-GGAC-GTGGG

Variant names:

• chr7-55181317-GAC-TGGG
• rs727503019
• NM_005228.5:c.2308_2310delGACinsTGGG

Variant summary

Our verdict is Pathogenic. The variant received 10 ACMG points: 10P and 0B. PVS1 PM2

The NM_005228.5(EGFR):​c.2308_2310delGACinsTGGG​(p.Asp770TrpfsTer127) variant causes a frameshift, missense change. The variant was absent in control chromosomes in GnomAD project. Variant has been reported in ClinVar as drug response (★). Another variant affecting the same amino acid position, but resulting in a different missense (i.e. D770N) has been classified as Likely benign. Variant results in nonsense mediated mRNA decay.

• Frequency: Genomes: not found (cov: 34)
• Consequence: EGFR NM_005228.5 frameshift, missense
• Clinical Significance: drug response criteria provided, single submitter O:1
• Conservation: PhyloP100: 6.90
• Publications: 0 publications found

Genes affected

EGFR (HGNC:3236): (epidermal growth factor receptor) The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). [provided by RefSeq, Jul 2020]

EGFR-AS1 (HGNC:40207): (EGFR antisense RNA 1)

ACMG classification

Our verdict: Pathogenic. The variant received 10 ACMG points.

• PVS1: Loss of function variant, product undergoes nonsense mediated mRNA decay. LoF is a known mechanism of disease.
• PM2: Very rare variant in population databases, with high coverage

Variant Effect in Transcripts

ACMG analysis was done for transcript: NM_005228.5. You can select a different transcript below to see updated ACMG assignments.

RefSeq Transcripts

Selected	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	EGFR	NM_005228.5	MANE Select	c.2308_2310delGACinsTGGG	p.Asp770TrpfsTer127	frameshift missense	Exon 20 of 28	NP_005219.2
	EGFR	NM_001346899.2		c.2173_2175delGACinsTGGG	p.Asp725TrpfsTer127	frameshift missense	Exon 19 of 27	NP_001333828.1
	EGFR	NM_001346900.2		c.2149_2151delGACinsTGGG	p.Asp717TrpfsTer127	frameshift missense	Exon 20 of 28	NP_001333829.1

Ensembl Transcripts

Selected	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	EGFR	ENST00000275493.7	TSL:1 MANE Select	c.2308_2310delGACinsTGGG	p.Asp770TrpfsTer127	frameshift missense	Exon 20 of 28	ENSP00000275493.2
	EGFR	ENST00000455089.5	TSL:1	c.2173_2175delGACinsTGGG	p.Asp725TrpfsTer127	frameshift missense	Exon 19 of 26	ENSP00000415559.1
	EGFR	ENST00000450046.2	TSL:4	c.2149_2151delGACinsTGGG	p.Asp717TrpfsTer127	frameshift missense	Exon 20 of 28	ENSP00000413354.2

Frequencies

GnomAD3 genomes Cov.: 34

We have no GnomAD4 exomes data on this position. Probably position not covered by the project.

GnomAD4 genome Cov.: 34

ClinVar

Significance: drug response

Submissions summary: Other:1

Revision: criteria provided, single submitter

Submissions by phenotype

Tyrosine kinase inhibitor response Other:1

Dec 06, 2012

Laboratory for Molecular Medicine, Mass General Brigham Personalized Medicine

Significance: drug response

Review Status: criteria provided, single submitter

Collection Method: clinical testing

Likely Resistant

Computational scores

Source: dbNSFP v4.9

Name	Calibrated prediction	Score	Prediction
PhyloP100		6.9

Splicing

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Other links and lift over

dbSNP: rs727503019; hg19: chr7-55249010;