9-27409266-T-G

Position:

• chr9-27409266-T-G

Variant summary

Our verdict is Benign. Variant got -12 ACMG points: 0P and 12B. BP4_Strong BA1

The NM_024761.5(MOB3B):​c.418+45867A>C variant causes a intron change involving the alteration of a non-conserved nucleotide. The variant allele was found at a frequency of 0.077 in 152,272 control chromosomes in the GnomAD database, including 529 homozygotes. In-silico tool predicts a benign outcome for this variant. No clinical diagnostic laboratories have submitted clinical-significance assessments for this variant to ClinVar.

• Frequency: Genomes: 𝑓 0.077 (529 hom., cov: 32)
• Consequence: MOB3B NM_024761.5 intron
• Clinical Significance: Not reported in ClinVar
• Conservation: PhyloP100: -1.32

Genes affected

MOB3B (HGNC:23825): (MOB kinase activator 3B) The protein encoded by this gene shares similarity with the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. This gene is located on the opposite strand as the interferon kappa precursor (IFNK) gene. [provided by RefSeq, Jul 2008]

ACMG classification

Verdict is Benign. Variant got -12 ACMG points.

• BP4: Computational evidence support a benign effect (BayesDel_noAF=-0.86).
• BA1: GnomAd4 highest subpopulation (SAS) allele frequency at 95% confidence interval = 0.116 is higher than 0.05.

Transcripts

RefSeq

Gene	Transcript	HGVSc	HGVSp	Effect	#exon/exons	MANE	UniProt
MOB3B	NM_024761.5	c.418+45867A>C		intron_variant		ENST00000262244.6

Ensembl

Gene	Transcript	HGVSc	HGVSp	Effect	#exon/exons	TSL	MANE	Appris	UniProt
MOB3B	ENST00000262244.6	c.418+45867A>C		intron_variant		1	NM_024761.5	P1
MOB3B	ENST00000603061.1	n.623+11826A>C		intron_variant, non_coding_transcript_variant		5

Frequencies

GnomAD3 genomes AF: 0.0769 AC: 11706 AN: 152154 Hom.: 526 Cov.: 32

Gnomad AFR AF: 0.0378

Gnomad AMI AF: 0.0208

Gnomad AMR AF: 0.0785

Gnomad ASJ AF: 0.0692

Gnomad EAS AF: 0.116

Gnomad SAS AF: 0.124

Gnomad FIN AF: 0.0940

Gnomad MID AF: 0.0348

Gnomad NFE AF: 0.0933

Gnomad OTH AF: 0.0597

We have no GnomAD4 exomes data on this position. Probably position not covered by the project.

GnomAD4 genome AF: 0.0770 AC: 11720 AN: 152272 Hom.: 529 Cov.: 32 AF XY: 0.0782 AC XY: 5820 AN XY: 74462

Gnomad4 AFR AF: 0.0379

Gnomad4 AMR AF: 0.0783

Gnomad4 ASJ AF: 0.0692

Gnomad4 EAS AF: 0.115

Gnomad4 SAS AF: 0.124

Gnomad4 FIN AF: 0.0940

Gnomad4 NFE AF: 0.0933

Gnomad4 OTH AF: 0.0647

Alfa AF: 0.0895 Hom.: 432

Bravo AF: 0.0718

Asia WGS AF: 0.121 AC: 421 AN: 3478

ClinVar

Not reported in ClinVar

Computational scores

Source: dbNSFP v4.3

Name	Calibrated prediction	Score	Prediction
BayesDel_noAF	Benign	-0.86
CADD	Benign	0.026
DANN	Benign	0.71

Splicing

Name	Calibrated prediction	Score	Prediction
SpliceAI score (max)		0.0		Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Publications

LitVar

Other links and lift over

dbSNP: rs2225389; hg19: chr9-27409264;