AGT Met259Thr

Variant summary

Our verdict is Benign. The variant received -20 ACMG points: 0P and 20B. BP4_StrongBP6_Very_StrongBA1

The NM_001384479.1(AGT):​c.776T>C​(p.Met259Thr) variant causes a missense change involving the alteration of a non-conserved nucleotide. The variant allele was found at a frequency of 0.469 in 1,613,874 control chromosomes in the GnomAD database, including 191,182 homozygotes. In-silico tool predicts a benign outcome for this variant. 16/22 in silico tools predict a benign outcome for this variant. Variant has been reported in ClinVar as Benign (★★).

Frequency

Genomes: 𝑓 0.58 ( 28034 hom., cov: 33)
Exomes 𝑓: 0.46 ( 163148 hom. )

Consequence

AGT
NM_001384479.1 missense

Scores

18

Clinical Significance

Benign criteria provided, multiple submitters, no conflicts B:10

Conservation

PhyloP100: 0.514
Variant links:
Genes affected
AGT (HGNC:333): (angiotensinogen) The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure, body fluid and electrolyte homeostasis, and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. [provided by RefSeq, Nov 2019]

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ACMG classification

Classification was made for transcript

Our verdict: Benign. The variant received -20 ACMG points.

BP4
Computational evidence support a benign effect (MetaRNN=8.121765E-7).
BP6
Variant 1-230710048-A-G is Benign according to our data. Variant chr1-230710048-A-G is described in ClinVar as [Benign]. Clinvar id is 18068.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars. Variant chr1-230710048-A-G is described in Lovd as [Benign].
BA1
GnomAd4 highest subpopulation (EAS) allele frequency at 95% confidence interval = 0.823 is higher than 0.05.

Transcripts

RefSeq

Gene Transcript HGVSc HGVSp Effect Exon rank MANE Protein UniProt
AGTNM_001384479.1 linkc.776T>C p.Met259Thr missense_variant Exon 2 of 5 ENST00000366667.6 NP_001371408.1
AGTNM_001382817.3 linkc.776T>C p.Met259Thr missense_variant Exon 2 of 5 NP_001369746.2

Ensembl

Gene Transcript HGVSc HGVSp Effect Exon rank TSL MANE Protein Appris UniProt
AGTENST00000366667.6 linkc.776T>C p.Met259Thr missense_variant Exon 2 of 5 1 NM_001384479.1 ENSP00000355627.5 P01019

Frequencies

GnomAD3 genomes
AF:
0.578
AC:
87869
AN:
152062
Hom.:
27970
Cov.:
33
show subpopulations
Gnomad AFR
AF:
0.830
Gnomad AMI
AF:
0.433
Gnomad AMR
AF:
0.631
Gnomad ASJ
AF:
0.430
Gnomad EAS
AF:
0.833
Gnomad SAS
AF:
0.623
Gnomad FIN
AF:
0.447
Gnomad MID
AF:
0.519
Gnomad NFE
AF:
0.421
Gnomad OTH
AF:
0.548
GnomAD2 exomes
AF:
0.548
AC:
137772
AN:
251344
AF XY:
0.536
show subpopulations
Gnomad AFR exome
AF:
0.845
Gnomad AMR exome
AF:
0.720
Gnomad ASJ exome
AF:
0.440
Gnomad EAS exome
AF:
0.839
Gnomad FIN exome
AF:
0.441
Gnomad NFE exome
AF:
0.420
Gnomad OTH exome
AF:
0.500
GnomAD4 exome
AF:
0.458
AC:
669469
AN:
1461692
Hom.:
163148
Cov.:
65
AF XY:
0.462
AC XY:
335636
AN XY:
727170
show subpopulations
Gnomad4 AFR exome
AF:
0.847
AC:
28371
AN:
33480
Gnomad4 AMR exome
AF:
0.709
AC:
31723
AN:
44724
Gnomad4 ASJ exome
AF:
0.434
AC:
11332
AN:
26134
Gnomad4 EAS exome
AF:
0.825
AC:
32760
AN:
39698
Gnomad4 SAS exome
AF:
0.615
AC:
53082
AN:
86250
Gnomad4 FIN exome
AF:
0.436
AC:
23270
AN:
53312
Gnomad4 NFE exome
AF:
0.410
AC:
455815
AN:
1111932
Gnomad4 Remaining exome
AF:
0.497
AC:
30028
AN:
60394
Heterozygous variant carriers
0
23181
46362
69542
92723
115904
0.00
0.20
0.40
0.60
0.80
0.95
Allele balance
Exome Het
Exome Hom
Variant carriers
0
14258
28516
42774
57032
71290
<30
30-35
35-40
40-45
45-50
50-55
55-60
60-65
65-70
70-75
75-80
>80
Age
GnomAD4 genome
AF:
0.578
AC:
87993
AN:
152182
Hom.:
28034
Cov.:
33
AF XY:
0.583
AC XY:
43390
AN XY:
74390
show subpopulations
Gnomad4 AFR
AF:
0.830
AC:
0.830437
AN:
0.830437
Gnomad4 AMR
AF:
0.632
AC:
0.63214
AN:
0.63214
Gnomad4 ASJ
AF:
0.430
AC:
0.429642
AN:
0.429642
Gnomad4 EAS
AF:
0.832
AC:
0.831781
AN:
0.831781
Gnomad4 SAS
AF:
0.622
AC:
0.621739
AN:
0.621739
Gnomad4 FIN
AF:
0.447
AC:
0.446931
AN:
0.446931
Gnomad4 NFE
AF:
0.421
AC:
0.420722
AN:
0.420722
Gnomad4 OTH
AF:
0.553
AC:
0.55298
AN:
0.55298
Heterozygous variant carriers
0
1761
3522
5284
7045
8806
0.00
0.20
0.40
0.60
0.80
0.95
Allele balance
Genome Het
Genome Hom
Variant carriers
0
720
1440
2160
2880
3600
<30
30-35
35-40
40-45
45-50
50-55
55-60
60-65
65-70
70-75
75-80
>80
Age
Alfa
AF:
0.474
Hom.:
91799
Bravo
AF:
0.604
TwinsUK
AF:
0.411
AC:
1525
ALSPAC
AF:
0.395
AC:
1524
ESP6500AA
AF:
0.827
AC:
3643
ESP6500EA
AF:
0.426
AC:
3662
ExAC
AF:
0.548
AC:
66560
Asia WGS
AF:
0.729
AC:
2532
AN:
3478
EpiCase
AF:
0.426
EpiControl
AF:
0.424

ClinVar

Significance: Benign
Submissions summary: Benign:10
Revision: criteria provided, multiple submitters, no conflicts
LINK: link

Submissions by phenotype

not specified Benign:3
-
Diagnostic Laboratory, Department of Genetics, University Medical Center Groningen
Significance:Benign
Review Status:no assertion criteria provided
Collection Method:clinical testing

- -

-
Joint Genome Diagnostic Labs from Nijmegen and Maastricht, Radboudumc and MUMC+
Significance:Benign
Review Status:no assertion criteria provided
Collection Method:clinical testing

- -

-
PreventionGenetics, part of Exact Sciences
Significance:Benign
Review Status:criteria provided, single submitter
Collection Method:clinical testing

- -

not provided Benign:3
Mar 13, 2018
GeneDx
Significance:Benign
Review Status:criteria provided, single submitter
Collection Method:clinical testing

This variant is considered likely benign or benign based on one or more of the following criteria: it is a conservative change, it occurs at a poorly conserved position in the protein, it is predicted to be benign by multiple in silico algorithms, and/or has population frequency not consistent with disease. -

-
Breakthrough Genomics, Breakthrough Genomics
Significance:Benign
Review Status:criteria provided, single submitter
Collection Method:not provided

- -

Feb 03, 2025
Labcorp Genetics (formerly Invitae), Labcorp
Significance:Benign
Review Status:criteria provided, single submitter
Collection Method:clinical testing

- -

Renal tubular dysgenesis Benign:2
Jul 14, 2021
Genome-Nilou Lab
Significance:Benign
Review Status:criteria provided, single submitter
Collection Method:clinical testing

- -

Mar 06, 2018
Illumina Laboratory Services, Illumina
Significance:Benign
Review Status:criteria provided, single submitter
Collection Method:clinical testing

This variant was observed in the ICSL laboratory as part of a predisposition screen in an ostensibly healthy population. It had not been previously curated by ICSL or reported in the Human Gene Mutation Database (HGMD: prior to June 1st, 2018), and was therefore a candidate for classification through an automated scoring system. Utilizing variant allele frequency, disease prevalence and penetrance estimates, and inheritance mode, an automated score was calculated to assess if this variant is too frequent to cause the disease. Based on the score and internal cut-off values, a variant classified as benign is not then subjected to further curation. The score for this variant resulted in a classification of benign for this disease. -

Hypertensive disorder Benign:1
Oct 12, 2021
iDNA Genomics
Significance:Benign
Review Status:no assertion criteria provided
Collection Method:reference population

- -

Hypertension, essential, susceptibility to Benign:1
Jan 01, 2005
OMIM
Significance:Benign
Review Status:no assertion criteria provided
Collection Method:literature only

- -

Computational scores

Source: dbNSFP v4.3

Name
Calibrated prediction
Score
Prediction
AlphaMissense
Benign
0.054
BayesDel_addAF
Benign
-0.63
T
BayesDel_noAF
Benign
-0.54
CADD
Benign
0.090
DANN
Benign
0.47
DEOGEN2
Benign
0.21
T
Eigen
Benign
-1.6
Eigen_PC
Benign
-1.5
FATHMM_MKL
Benign
0.0028
N
LIST_S2
Benign
0.10
T
MetaRNN
Benign
8.1e-7
T
MetaSVM
Benign
-0.99
T
MutationAssessor
Benign
-1.7
N
PrimateAI
Benign
0.26
T
PROVEAN
Benign
1.6
N
REVEL
Benign
0.16
Sift
Benign
1.0
T
Sift4G
Benign
1.0
T
Polyphen
0.0
B
Vest4
0.024
MPC
0.054
ClinPred
0.0090
T
GERP RS
-0.37
RBP_binding_hub_radar
0.0
RBP_regulation_power_radar
1.1
Varity_R
0.18
gMVP
0.14
Mutation Taster
=98/2
polymorphism (auto)

Splicing

Name
Calibrated prediction
Score
Prediction
SpliceAI score (max)
0.0
Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Publications

LitVar

Below is the list of publications found by LitVar. It may be empty.

Other links and lift over

dbSNP: rs699; hg19: chr1-230845794; COSMIC: COSV64184214; COSMIC: COSV64184214; API