X-101656961-C-G

Variant names:

• chrX-101656961-C-G
• rs137976844
• NM_177949.4:c.628G>C

Variant summary

Our verdict is Benign. The variant received -8 ACMG points: 0P and 8B. BP4_Strong BS2

The NM_177949.4(ARMCX2):​c.628G>C​(p.Gly210Arg) variant causes a missense change involving the alteration of a non-conserved nucleotide. The variant allele was found at a frequency of 0.000742 in 1,209,567 control chromosomes in the GnomAD database, with no homozygous occurrence. There are 273 hemizygotes in GnomAD. In-silico tool predicts a benign outcome for this variant. 15/21 in silico tools predict a benign outcome for this variant. Variant has been reported in ClinVar as Uncertain significance (★). Another nucleotide change resulting in the same amino acid substitution has been previously reported as Uncertain significance in ClinVar.

• Frequency:
  • Genomes: 𝑓 0.00059 (0 hom., 14 hem., cov: 24)
  • Exomes 𝑓: 0.00076 (0 hom. 259 hem.)
• Consequence: ARMCX2 NM_177949.4 missense
• Clinical Significance: Uncertain significance criteria provided, single submitter U:1 B:2
• Conservation: PhyloP100: -2.27
• Publications: 0 publications found

Genes affected

ARMCX2 (HGNC:16869): (armadillo repeat containing X-linked 2) This gene encodes a protein containing a potential N-terminal transmembrane domain and multiple armadillo (arm) repeats. Proteins containing arm repeats are involved in development, maintenance of tissue integrity, and tumorigenesis. This gene is located in a cluster of related genes on chromosome X. There is a pseudogene for this gene on chromosome 7. Alternative splicing in the 5' UTR results in multiple transcript variants encoding the same protein. [provided by RefSeq, Aug 2013]

ACMG classification

Our verdict: Benign. The variant received -8 ACMG points.

• BP4: Computational evidence support a benign effect (MetaRNN=0.0040902793).
• BS2: High Hemizygotes in GnomAd4 at 14 gene

Variant Effect in Transcripts

ACMG analysis was done for transcript: NM_177949.4. You can select a different transcript below to see updated ACMG assignments.

RefSeq Transcripts

Sel.	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	ARMCX2	NM_177949.4	MANE Select	c.628G>C	p.Gly210Arg	missense	Exon 6 of 6	NP_808818.1	Q7L311
	ARMCX2	NM_001282231.2		c.628G>C	p.Gly210Arg	missense	Exon 6 of 6	NP_001269160.1	Q7L311
	ARMCX2	NM_014782.7		c.628G>C	p.Gly210Arg	missense	Exon 5 of 5	NP_055597.1	Q7L311

Ensembl Transcripts

Sel.	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	ARMCX2	ENST00000356824.9	TSL:1 MANE Select	c.628G>C	p.Gly210Arg	missense	Exon 6 of 6	ENSP00000349281.4	Q7L311
	ARMCX2	ENST00000328766.9	TSL:1	c.628G>C	p.Gly210Arg	missense	Exon 5 of 5	ENSP00000331662.5	Q7L311
	ARMCX2	ENST00000330154.6	TSL:1	c.628G>C	p.Gly210Arg	missense	Exon 3 of 3	ENSP00000328631.2	Q7L311

Frequencies

GnomAD3 genomes AF: 0.000591 AC: 66 AN: 111627 Hom.: 0 Cov.: 24

Gnomad AFR AF: 0.0000326

Gnomad AMI AF: 0.0234

Gnomad AMR AF: 0.000188

Gnomad ASJ AF: 0.00

Gnomad EAS AF: 0.00

Gnomad SAS AF: 0.00

Gnomad FIN AF: 0.00

Gnomad MID AF: 0.00

Gnomad NFE AF: 0.000888

Gnomad OTH AF: 0.00

GnomAD2 exomes AF: 0.000439 AC: 79 AN: 179970 AF XY: 0.000394

Gnomad AFR exome AF: 0.0000790

Gnomad AMR exome AF: 0.000219

Gnomad ASJ exome AF: 0.00

Gnomad EAS exome AF: 0.00

Gnomad FIN exome AF: 0.00

Gnomad NFE exome AF: 0.000905

Gnomad OTH exome AF: 0.00

GnomAD4 exome AF: 0.000757 AC: 831 AN: 1097940 Hom.: 0 Cov.: 34 AF XY: 0.000713 AC XY: 259 AN XY: 363346

African (AFR) AF: 0.0000757 AC: 2 AN: 26403

American (AMR) AF: 0.000256 AC: 9 AN: 35205

Ashkenazi Jewish (ASJ) AF: 0.00 AC: 0 AN: 19384

East Asian (EAS) AF: 0.00 AC: 0 AN: 30198

South Asian (SAS) AF: 0.00 AC: 0 AN: 54132

European-Finnish (FIN) AF: 0.00 AC: 0 AN: 40291

Middle Eastern (MID) AF: 0.00 AC: 0 AN: 4124

European-Non Finnish (NFE) AF: 0.000944 AC: 795 AN: 842116

Other (OTH) AF: 0.000542 AC: 25 AN: 46087

GnomAD4 genome AF: 0.000591 AC: 66 AN: 111627 Hom.: 0 Cov.: 24 AF XY: 0.000414 AC XY: 14 AN XY: 33805

African (AFR) AF: 0.0000326 AC: 1 AN: 30661

American (AMR) AF: 0.000188 AC: 2 AN: 10664

Ashkenazi Jewish (ASJ) AF: 0.00 AC: 0 AN: 2642

East Asian (EAS) AF: 0.00 AC: 0 AN: 3512

South Asian (SAS) AF: 0.00 AC: 0 AN: 2644

European-Finnish (FIN) AF: 0.00 AC: 0 AN: 6121

Middle Eastern (MID) AF: 0.00 AC: 0 AN: 238

European-Non Finnish (NFE) AF: 0.000888 AC: 47 AN: 52955

Other (OTH) AF: 0.00 AC: 0 AN: 1507

Alfa AF: 0.0000434 Hom.: 0

Bravo AF: 0.000574

TwinsUK AF: 0.000539 AC: 2

ALSPAC AF: 0.00138 AC: 4

ExAC AF: 0.000495 AC: 60

EpiCase AF: 0.000872

EpiControl AF: 0.000712

ClinVar

ClinVar submissions

Significance: Uncertain significance

Revision: criteria provided, single submitter

Pathogenic	VUS	Benign	Condition
-	-	2	not provided (2)
-	1	-	not specified (1)

Computational scores

Source: dbNSFP v4.9

Name	Calibrated prediction	Score	Prediction
AlphaMissense	Benign	0.075
BayesDel_addAF	Benign	-0.90	T
BayesDel_noAF	Benign	-1.1
CADD	Benign	0.027
DANN	Benign	0.23
DEOGEN2	Benign	0.013	T
FATHMM_MKL	Benign	0.049	N
LIST_S2	Benign	0.37	T
M_CAP	Benign	0.021	T
MetaRNN	Benign	0.0041	T
MetaSVM	Benign	-1.0	T
MutationAssessor	Benign	0.81	L
PhyloP100		-2.3
PrimateAI	Benign	0.42	T
PROVEAN	Benign	-1.3	N
REVEL	Benign	0.0070
Sift	Benign	1.0	T
Sift4G	Benign	0.15	T
Polyphen		0.0	B
Vest4		0.049
MutPred		0.27		Loss of catalytic residue at V211 (P = 0.0398)
MVP		0.068
MPC		0.45
ClinPred		0.0043	T
GERP RS		-6.3
Varity_R		0.052
gMVP		0.072
Mutation Taster		=100/0	polymorphism

Splicing

Name	Calibrated prediction	Score	Prediction
SpliceAI score (max)		0.0		Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Other links and lift over

dbSNP: rs137976844; hg19: chrX-100911947;