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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-155947968-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=155947968&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 155947968,
      "ref": "C",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001162383.2",
      "consequences": [
        {
          "aa_ref": "G",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF2",
          "gene_hgnc_id": 682,
          "hgvs_c": "c.2935G>T",
          "hgvs_p": "p.Gly979Trp",
          "transcript": "NM_001162383.2",
          "protein_id": "NP_001155855.1",
          "transcript_support_level": null,
          "aa_start": 979,
          "aa_end": null,
          "aa_length": 986,
          "cds_start": 2935,
          "cds_end": null,
          "cds_length": 2961,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000361247.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001162383.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "W",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF2",
          "gene_hgnc_id": 682,
          "hgvs_c": "c.2935G>T",
          "hgvs_p": "p.Gly979Trp",
          "transcript": "ENST00000361247.9",
          "protein_id": "ENSP00000354837.4",
          "transcript_support_level": 1,
          "aa_start": 979,
          "aa_end": null,
          "aa_length": 986,
          "cds_start": 2935,
          "cds_end": null,
          "cds_length": 2961,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001162383.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000361247.9"
        },
        {
          "aa_ref": "G",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF2",
          "gene_hgnc_id": 682,
          "hgvs_c": "c.2932G>T",
          "hgvs_p": "p.Gly978Trp",
          "transcript": "ENST00000313667.8",
          "protein_id": "ENSP00000314787.4",
          "transcript_support_level": 1,
          "aa_start": 978,
          "aa_end": null,
          "aa_length": 985,
          "cds_start": 2932,
          "cds_end": null,
          "cds_length": 2958,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000313667.8"
        },
        {
          "aa_ref": "G",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF2",
          "gene_hgnc_id": 682,
          "hgvs_c": "c.2851G>T",
          "hgvs_p": "p.Gly951Trp",
          "transcript": "ENST00000313695.11",
          "protein_id": "ENSP00000315325.7",
          "transcript_support_level": 1,
          "aa_start": 951,
          "aa_end": null,
          "aa_length": 958,
          "cds_start": 2851,
          "cds_end": null,
          "cds_length": 2877,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000313695.11"
        },
        {
          "aa_ref": "G",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF2",
          "gene_hgnc_id": 682,
          "hgvs_c": "c.3364G>T",
          "hgvs_p": "p.Gly1122Trp",
          "transcript": "ENST00000673475.1",
          "protein_id": "ENSP00000500802.1",
          "transcript_support_level": null,
          "aa_start": 1122,
          "aa_end": null,
          "aa_length": 1129,
          "cds_start": 3364,
          "cds_end": null,
          "cds_length": 3390,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000673475.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF2",
          "gene_hgnc_id": 682,
          "hgvs_c": "c.3070G>T",
          "hgvs_p": "p.Gly1024Trp",
          "transcript": "ENST00000462460.6",
          "protein_id": "ENSP00000476916.1",
          "transcript_support_level": 5,
          "aa_start": 1024,
          "aa_end": null,
          "aa_length": 1031,
          "cds_start": 3070,
          "cds_end": null,
          "cds_length": 3096,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000462460.6"
        },
        {
          "aa_ref": "G",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF2",
          "gene_hgnc_id": 682,
          "hgvs_c": "c.2938G>T",
          "hgvs_p": "p.Gly980Trp",
          "transcript": "ENST00000696601.1",
          "protein_id": "ENSP00000512746.1",
          "transcript_support_level": null,
          "aa_start": 980,
          "aa_end": null,
          "aa_length": 987,
          "cds_start": 2938,
          "cds_end": null,
          "cds_length": 2964,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000696601.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF2",
          "gene_hgnc_id": 682,
          "hgvs_c": "c.2932G>T",
          "hgvs_p": "p.Gly978Trp",
          "transcript": "NM_001162384.2",
          "protein_id": "NP_001155856.1",
          "transcript_support_level": null,
          "aa_start": 978,
          "aa_end": null,
          "aa_length": 985,
          "cds_start": 2932,
          "cds_end": null,
          "cds_length": 2958,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001162384.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF2",
          "gene_hgnc_id": 682,
          "hgvs_c": "c.2929G>T",
          "hgvs_p": "p.Gly977Trp",
          "transcript": "ENST00000873828.1",
          "protein_id": "ENSP00000543887.1",
          "transcript_support_level": null,
          "aa_start": 977,
          "aa_end": null,
          "aa_length": 984,
          "cds_start": 2929,
          "cds_end": null,
          "cds_length": 2955,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000873828.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF2",
          "gene_hgnc_id": 682,
          "hgvs_c": "c.2929G>T",
          "hgvs_p": "p.Gly977Trp",
          "transcript": "ENST00000873829.1",
          "protein_id": "ENSP00000543888.1",
          "transcript_support_level": null,
          "aa_start": 977,
          "aa_end": null,
          "aa_length": 984,
          "cds_start": 2929,
          "cds_end": null,
          "cds_length": 2955,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000873829.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF2",
          "gene_hgnc_id": 682,
          "hgvs_c": "c.2911G>T",
          "hgvs_p": "p.Gly971Trp",
          "transcript": "NM_001350112.2",
          "protein_id": "NP_001337041.1",
          "transcript_support_level": null,
          "aa_start": 971,
          "aa_end": null,
          "aa_length": 978,
          "cds_start": 2911,
          "cds_end": null,
          "cds_length": 2937,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001350112.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF2",
          "gene_hgnc_id": 682,
          "hgvs_c": "c.2884G>T",
          "hgvs_p": "p.Gly962Trp",
          "transcript": "NM_001350110.2",
          "protein_id": "NP_001337039.1",
          "transcript_support_level": null,
          "aa_start": 962,
          "aa_end": null,
          "aa_length": 969,
          "cds_start": 2884,
          "cds_end": null,
          "cds_length": 2910,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001350110.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF2",
          "gene_hgnc_id": 682,
          "hgvs_c": "c.2884G>T",
          "hgvs_p": "p.Gly962Trp",
          "transcript": "NM_001350111.2",
          "protein_id": "NP_001337040.1",
          "transcript_support_level": null,
          "aa_start": 962,
          "aa_end": null,
          "aa_length": 969,
          "cds_start": 2884,
          "cds_end": null,
          "cds_length": 2910,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001350111.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF2",
          "gene_hgnc_id": 682,
          "hgvs_c": "c.2851G>T",
          "hgvs_p": "p.Gly951Trp",
          "transcript": "NM_004723.4",
          "protein_id": "NP_004714.2",
          "transcript_support_level": null,
          "aa_start": 951,
          "aa_end": null,
          "aa_length": 958,
          "cds_start": 2851,
          "cds_end": null,
          "cds_length": 2877,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004723.4"
        },
        {
          "aa_ref": "G",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF2",
          "gene_hgnc_id": 682,
          "hgvs_c": "c.2686G>T",
          "hgvs_p": "p.Gly896Trp",
          "transcript": "ENST00000927873.1",
          "protein_id": "ENSP00000597932.1",
          "transcript_support_level": null,
          "aa_start": 896,
          "aa_end": null,
          "aa_length": 903,
          "cds_start": 2686,
          "cds_end": null,
          "cds_length": 2712,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000927873.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF2",
          "gene_hgnc_id": 682,
          "hgvs_c": "c.763G>T",
          "hgvs_p": "p.Gly255Trp",
          "transcript": "ENST00000470541.6",
          "protein_id": "ENSP00000476689.1",
          "transcript_support_level": 3,
          "aa_start": 255,
          "aa_end": null,
          "aa_length": 262,
          "cds_start": 763,
          "cds_end": null,
          "cds_length": 789,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000470541.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF2",
          "gene_hgnc_id": 682,
          "hgvs_c": "n.*2597G>T",
          "hgvs_p": null,
          "transcript": "ENST00000696600.1",
          "protein_id": "ENSP00000512745.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000696600.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF2",
          "gene_hgnc_id": 682,
          "hgvs_c": "n.*2597G>T",
          "hgvs_p": null,
          "transcript": "ENST00000696600.1",
          "protein_id": "ENSP00000512745.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000696600.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF2",
          "gene_hgnc_id": 682,
          "hgvs_c": "n.658-888G>T",
          "hgvs_p": null,
          "transcript": "ENST00000477754.2",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000477754.2"
        }
      ],
      "gene_symbol": "ARHGEF2",
      "gene_hgnc_id": 682,
      "dbsnp": "rs200032144",
      "frequency_reference_population": 0.00020372796,
      "hom_count_reference_population": 2,
      "allele_count_reference_population": 316,
      "gnomad_exomes_af": 0.000212337,
      "gnomad_genomes_af": 0.000124696,
      "gnomad_exomes_ac": 297,
      "gnomad_genomes_ac": 19,
      "gnomad_exomes_homalt": 2,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.08319789171218872,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.274,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.2192,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.14,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 4.356,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -6,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate,BS2",
      "acmg_by_gene": [
        {
          "score": -6,
          "benign_score": 6,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001162383.2",
          "gene_symbol": "ARHGEF2",
          "hgnc_id": 682,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2935G>T",
          "hgvs_p": "p.Gly979Trp"
        }
      ],
      "clinvar_disease": "Microcephaly",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "Microcephaly",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}