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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 22-41584362-T-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=22&pos=41584362&ref=T&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "22",
      "pos": 41584362,
      "ref": "T",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_002676.3",
      "consequences": [
        {
          "aa_ref": "N",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.293A>T",
          "hgvs_p": "p.Asn98Ile",
          "transcript": "NM_002676.3",
          "protein_id": "NP_002667.2",
          "transcript_support_level": null,
          "aa_start": 98,
          "aa_end": null,
          "aa_length": 262,
          "cds_start": 293,
          "cds_end": null,
          "cds_length": 789,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000216259.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002676.3"
        },
        {
          "aa_ref": "N",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.293A>T",
          "hgvs_p": "p.Asn98Ile",
          "transcript": "ENST00000216259.8",
          "protein_id": "ENSP00000216259.7",
          "transcript_support_level": 1,
          "aa_start": 98,
          "aa_end": null,
          "aa_length": 262,
          "cds_start": 293,
          "cds_end": null,
          "cds_length": 789,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_002676.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000216259.8"
        },
        {
          "aa_ref": "N",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.527A>T",
          "hgvs_p": "p.Asn176Ile",
          "transcript": "ENST00000949539.1",
          "protein_id": "ENSP00000619598.1",
          "transcript_support_level": null,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 340,
          "cds_start": 527,
          "cds_end": null,
          "cds_length": 1023,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000949539.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.422A>T",
          "hgvs_p": "p.Asn141Ile",
          "transcript": "ENST00000940470.1",
          "protein_id": "ENSP00000610529.1",
          "transcript_support_level": null,
          "aa_start": 141,
          "aa_end": null,
          "aa_length": 305,
          "cds_start": 422,
          "cds_end": null,
          "cds_length": 918,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000940470.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.293A>T",
          "hgvs_p": "p.Asn98Ile",
          "transcript": "ENST00000949538.1",
          "protein_id": "ENSP00000619597.1",
          "transcript_support_level": null,
          "aa_start": 98,
          "aa_end": null,
          "aa_length": 277,
          "cds_start": 293,
          "cds_end": null,
          "cds_length": 834,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000949538.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.293A>T",
          "hgvs_p": "p.Asn98Ile",
          "transcript": "ENST00000949537.1",
          "protein_id": "ENSP00000619596.1",
          "transcript_support_level": null,
          "aa_start": 98,
          "aa_end": null,
          "aa_length": 271,
          "cds_start": 293,
          "cds_end": null,
          "cds_length": 816,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000949537.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.314A>T",
          "hgvs_p": "p.Asn105Ile",
          "transcript": "ENST00000896620.1",
          "protein_id": "ENSP00000566679.1",
          "transcript_support_level": null,
          "aa_start": 105,
          "aa_end": null,
          "aa_length": 269,
          "cds_start": 314,
          "cds_end": null,
          "cds_length": 810,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000896620.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.293A>T",
          "hgvs_p": "p.Asn98Ile",
          "transcript": "ENST00000949536.1",
          "protein_id": "ENSP00000619595.1",
          "transcript_support_level": null,
          "aa_start": 98,
          "aa_end": null,
          "aa_length": 262,
          "cds_start": 293,
          "cds_end": null,
          "cds_length": 789,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000949536.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.293A>T",
          "hgvs_p": "p.Asn98Ile",
          "transcript": "ENST00000940468.1",
          "protein_id": "ENSP00000610527.1",
          "transcript_support_level": null,
          "aa_start": 98,
          "aa_end": null,
          "aa_length": 261,
          "cds_start": 293,
          "cds_end": null,
          "cds_length": 786,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000940468.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.293A>T",
          "hgvs_p": "p.Asn98Ile",
          "transcript": "ENST00000940469.1",
          "protein_id": "ENSP00000610528.1",
          "transcript_support_level": null,
          "aa_start": 98,
          "aa_end": null,
          "aa_length": 260,
          "cds_start": 293,
          "cds_end": null,
          "cds_length": 783,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000940469.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.287A>T",
          "hgvs_p": "p.Asn96Ile",
          "transcript": "ENST00000940471.1",
          "protein_id": "ENSP00000610530.1",
          "transcript_support_level": null,
          "aa_start": 96,
          "aa_end": null,
          "aa_length": 260,
          "cds_start": 287,
          "cds_end": null,
          "cds_length": 783,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000940471.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.260A>T",
          "hgvs_p": "p.Asn87Ile",
          "transcript": "ENST00000896617.1",
          "protein_id": "ENSP00000566676.1",
          "transcript_support_level": null,
          "aa_start": 87,
          "aa_end": null,
          "aa_length": 251,
          "cds_start": 260,
          "cds_end": null,
          "cds_length": 756,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000896617.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.293A>T",
          "hgvs_p": "p.Asn98Ile",
          "transcript": "ENST00000896616.1",
          "protein_id": "ENSP00000566675.1",
          "transcript_support_level": null,
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          "aa_end": null,
          "aa_length": 240,
          "cds_start": 293,
          "cds_end": null,
          "cds_length": 723,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000896616.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.308A>T",
          "hgvs_p": "p.Asn103Ile",
          "transcript": "XM_011530230.3",
          "protein_id": "XP_011528532.1",
          "transcript_support_level": null,
          "aa_start": 103,
          "aa_end": null,
          "aa_length": 267,
          "cds_start": 308,
          "cds_end": null,
          "cds_length": 804,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "N",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.284A>T",
          "hgvs_p": "p.Asn95Ile",
          "transcript": "ENST00000896615.1",
          "protein_id": "ENSP00000566674.1",
          "transcript_support_level": null,
          "aa_start": 95,
          "aa_end": null,
          "aa_length": 259,
          "cds_start": 284,
          "cds_end": null,
          "cds_length": 780,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000896615.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.-53A>T",
          "hgvs_p": null,
          "transcript": "XM_047441411.1",
          "protein_id": "XP_047297367.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 444,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047441411.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.282+165A>T",
          "hgvs_p": null,
          "transcript": "ENST00000896618.1",
          "protein_id": "ENSP00000566677.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 206,
          "cds_start": null,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
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          "canonical": false,
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          "strand": false,
          "consequences": [
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          ],
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          "exon_count": 6,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.164+165A>T",
          "hgvs_p": null,
          "transcript": "ENST00000896619.1",
          "protein_id": "ENSP00000566678.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": 192,
          "cds_start": null,
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          "cds_length": 579,
          "cdna_start": null,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000896619.1"
        },
        {
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          "canonical": false,
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          "consequences": [
            "intron_variant"
          ],
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          "exon_count": 4,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.87+5357A>T",
          "hgvs_p": null,
          "transcript": "ENST00000940472.1",
          "protein_id": "ENSP00000610531.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": 133,
          "cds_start": null,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000940472.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 5,
          "intron_rank_end": null,
          "gene_symbol": "PMM1",
          "gene_hgnc_id": 9114,
          "hgvs_c": "c.503+165A>T",
          "hgvs_p": null,
          "transcript": "XM_011530229.3",
          "protein_id": "XP_011528531.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 305,
          "cds_start": null,
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          "cds_length": 918,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011530229.3"
        },
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        {
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        {
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000414636.1"
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      ],
      "gene_symbol": "PMM1",
      "gene_hgnc_id": 9114,
      "dbsnp": "rs1569056232",
      "frequency_reference_population": 6.840797e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.8408e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.7291097044944763,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.05999999865889549,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.682,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.2185,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": 0.02,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 1.569,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.06,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_002676.3",
          "gene_symbol": "PMM1",
          "hgnc_id": 9114,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.293A>T",
          "hgvs_p": "p.Asn98Ile"
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}