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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-31780526-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=31780526&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 31780526,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_006295.3",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.2840G>A",
          "hgvs_p": "p.Arg947His",
          "transcript": "NM_006295.3",
          "protein_id": "NP_006286.1",
          "transcript_support_level": null,
          "aa_start": 947,
          "aa_end": null,
          "aa_length": 1264,
          "cds_start": 2840,
          "cds_end": null,
          "cds_length": 3795,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000375663.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_006295.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.2840G>A",
          "hgvs_p": "p.Arg947His",
          "transcript": "ENST00000375663.8",
          "protein_id": "ENSP00000364815.3",
          "transcript_support_level": 1,
          "aa_start": 947,
          "aa_end": null,
          "aa_length": 1264,
          "cds_start": 2840,
          "cds_end": null,
          "cds_length": 3795,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_006295.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000375663.8"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.2885G>A",
          "hgvs_p": "p.Arg962His",
          "transcript": "ENST00000851851.1",
          "protein_id": "ENSP00000521910.1",
          "transcript_support_level": null,
          "aa_start": 962,
          "aa_end": null,
          "aa_length": 1279,
          "cds_start": 2885,
          "cds_end": null,
          "cds_length": 3840,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851851.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.2879G>A",
          "hgvs_p": "p.Arg960His",
          "transcript": "ENST00000851849.1",
          "protein_id": "ENSP00000521908.1",
          "transcript_support_level": null,
          "aa_start": 960,
          "aa_end": null,
          "aa_length": 1277,
          "cds_start": 2879,
          "cds_end": null,
          "cds_length": 3834,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851849.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.2873G>A",
          "hgvs_p": "p.Arg958His",
          "transcript": "ENST00000941400.1",
          "protein_id": "ENSP00000611459.1",
          "transcript_support_level": null,
          "aa_start": 958,
          "aa_end": null,
          "aa_length": 1275,
          "cds_start": 2873,
          "cds_end": null,
          "cds_length": 3828,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000941400.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.2843G>A",
          "hgvs_p": "p.Arg948His",
          "transcript": "ENST00000851848.1",
          "protein_id": "ENSP00000521907.1",
          "transcript_support_level": null,
          "aa_start": 948,
          "aa_end": null,
          "aa_length": 1265,
          "cds_start": 2843,
          "cds_end": null,
          "cds_length": 3798,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851848.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.2837G>A",
          "hgvs_p": "p.Arg946His",
          "transcript": "ENST00000925769.1",
          "protein_id": "ENSP00000595828.1",
          "transcript_support_level": null,
          "aa_start": 946,
          "aa_end": null,
          "aa_length": 1263,
          "cds_start": 2837,
          "cds_end": null,
          "cds_length": 3792,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925769.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.2840G>A",
          "hgvs_p": "p.Arg947His",
          "transcript": "ENST00000941401.1",
          "protein_id": "ENSP00000611460.1",
          "transcript_support_level": null,
          "aa_start": 947,
          "aa_end": null,
          "aa_length": 1261,
          "cds_start": 2840,
          "cds_end": null,
          "cds_length": 3786,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000941401.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.2840G>A",
          "hgvs_p": "p.Arg947His",
          "transcript": "ENST00000925773.1",
          "protein_id": "ENSP00000595832.1",
          "transcript_support_level": null,
          "aa_start": 947,
          "aa_end": null,
          "aa_length": 1260,
          "cds_start": 2840,
          "cds_end": null,
          "cds_length": 3783,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925773.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.2828G>A",
          "hgvs_p": "p.Arg943His",
          "transcript": "ENST00000925774.1",
          "protein_id": "ENSP00000595833.1",
          "transcript_support_level": null,
          "aa_start": 943,
          "aa_end": null,
          "aa_length": 1260,
          "cds_start": 2828,
          "cds_end": null,
          "cds_length": 3783,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925774.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.2825G>A",
          "hgvs_p": "p.Arg942His",
          "transcript": "ENST00000925775.1",
          "protein_id": "ENSP00000595834.1",
          "transcript_support_level": null,
          "aa_start": 942,
          "aa_end": null,
          "aa_length": 1259,
          "cds_start": 2825,
          "cds_end": null,
          "cds_length": 3780,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925775.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.2822G>A",
          "hgvs_p": "p.Arg941His",
          "transcript": "ENST00000925772.1",
          "protein_id": "ENSP00000595831.1",
          "transcript_support_level": null,
          "aa_start": 941,
          "aa_end": null,
          "aa_length": 1258,
          "cds_start": 2822,
          "cds_end": null,
          "cds_length": 3777,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925772.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.2816G>A",
          "hgvs_p": "p.Arg939His",
          "transcript": "ENST00000925770.1",
          "protein_id": "ENSP00000595829.1",
          "transcript_support_level": null,
          "aa_start": 939,
          "aa_end": null,
          "aa_length": 1256,
          "cds_start": 2816,
          "cds_end": null,
          "cds_length": 3771,
          "cdna_start": null,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925770.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.2714G>A",
          "hgvs_p": "p.Arg905His",
          "transcript": "ENST00000925771.1",
          "protein_id": "ENSP00000595830.1",
          "transcript_support_level": null,
          "aa_start": 905,
          "aa_end": null,
          "aa_length": 1222,
          "cds_start": 2714,
          "cds_end": null,
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        },
        {
          "aa_ref": "R",
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          ],
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          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.2705G>A",
          "hgvs_p": "p.Arg902His",
          "transcript": "ENST00000851850.1",
          "protein_id": "ENSP00000521909.1",
          "transcript_support_level": null,
          "aa_start": 902,
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          "aa_length": 1219,
          "cds_start": 2705,
          "cds_end": null,
          "cds_length": 3660,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851850.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.2705G>A",
          "hgvs_p": "p.Arg902His",
          "transcript": "ENST00000925776.1",
          "protein_id": "ENSP00000595835.1",
          "transcript_support_level": null,
          "aa_start": 902,
          "aa_end": null,
          "aa_length": 1219,
          "cds_start": 2705,
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          "biotype": "protein_coding",
          "feature": "ENST00000925776.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.2681G>A",
          "hgvs_p": "p.Arg894His",
          "transcript": "ENST00000925768.1",
          "protein_id": "ENSP00000595827.1",
          "transcript_support_level": null,
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        },
        {
          "aa_ref": "R",
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          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.791G>A",
          "hgvs_p": "p.Arg264His",
          "transcript": "ENST00000428445.1",
          "protein_id": "ENSP00000415679.1",
          "transcript_support_level": 5,
          "aa_start": 264,
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          "aa_length": 286,
          "cds_start": 791,
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          "cds_length": 862,
          "cdna_start": null,
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          "biotype": "protein_coding",
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        {
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          "strand": false,
          "consequences": [
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          ],
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          "exon_count": 30,
          "intron_rank": null,
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          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.2843G>A",
          "hgvs_p": "p.Arg948His",
          "transcript": "XM_005249362.3",
          "protein_id": "XP_005249419.1",
          "transcript_support_level": null,
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          "aa_length": 1265,
          "cds_start": 2843,
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          "cds_length": 3798,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_005249362.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.2843G>A",
          "hgvs_p": "p.Arg948His",
          "transcript": "XM_047419296.1",
          "protein_id": "XP_047275252.1",
          "transcript_support_level": null,
          "aa_start": 948,
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          "aa_length": 1265,
          "cds_start": 2843,
          "cds_end": null,
          "cds_length": 3798,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047419296.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "c.2840G>A",
          "hgvs_p": "p.Arg947His",
          "transcript": "XM_047419297.1",
          "protein_id": "XP_047275253.1",
          "transcript_support_level": null,
          "aa_start": 947,
          "aa_end": null,
          "aa_length": 1264,
          "cds_start": 2840,
          "cds_end": null,
          "cds_length": 3795,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047419297.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VARS1",
          "gene_hgnc_id": 12651,
          "hgvs_c": "n.*25G>A",
          "hgvs_p": null,
          "transcript": "ENST00000482996.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000482996.5"
        }
      ],
      "gene_symbol": "VARS1",
      "gene_hgnc_id": 12651,
      "dbsnp": "rs150882285",
      "frequency_reference_population": 0.000004956918,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 8,
      "gnomad_exomes_af": 0.00000342069,
      "gnomad_genomes_af": 0.0000197094,
      "gnomad_exomes_ac": 5,
      "gnomad_genomes_ac": 3,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8697041273117065,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.517,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.5694,
      "alphamissense_prediction": "Pathogenic",
      "bayesdelnoaf_score": -0.01,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 7.244,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 7,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM1,PM2,PP3_Moderate,PP5",
      "acmg_by_gene": [
        {
          "score": 7,
          "benign_score": 0,
          "pathogenic_score": 7,
          "criteria": [
            "PM1",
            "PM2",
            "PP3_Moderate",
            "PP5"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_006295.3",
          "gene_symbol": "VARS1",
          "hgnc_id": 12651,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2840G>A",
          "hgvs_p": "p.Arg947His"
        }
      ],
      "clinvar_disease": " and cortical atrophy, seizures,Neurodevelopmental disorder with microcephaly,not provided",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "LP:1 US:1",
      "phenotype_combined": "Neurodevelopmental disorder with microcephaly, seizures, and cortical atrophy|not provided",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}