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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 8-42856296-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=8&pos=42856296&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "8",
      "pos": 42856296,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "ENST00000527424.6",
      "consequences": [
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "c.640A>G",
          "hgvs_p": "p.Ile214Val",
          "transcript": "NM_030954.4",
          "protein_id": "NP_112216.3",
          "transcript_support_level": null,
          "aa_start": 214,
          "aa_end": null,
          "aa_length": 258,
          "cds_start": 640,
          "cds_end": null,
          "cds_length": 777,
          "cdna_start": 769,
          "cdna_end": null,
          "cdna_length": 3764,
          "mane_select": "ENST00000527424.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "c.640A>G",
          "hgvs_p": "p.Ile214Val",
          "transcript": "ENST00000527424.6",
          "protein_id": "ENSP00000434797.1",
          "transcript_support_level": 1,
          "aa_start": 214,
          "aa_end": null,
          "aa_length": 258,
          "cds_start": 640,
          "cds_end": null,
          "cds_length": 777,
          "cdna_start": 769,
          "cdna_end": null,
          "cdna_length": 3764,
          "mane_select": "NM_030954.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "c.640A>G",
          "hgvs_p": "p.Ile214Val",
          "transcript": "ENST00000534961.5",
          "protein_id": "ENSP00000445725.1",
          "transcript_support_level": 1,
          "aa_start": 214,
          "aa_end": null,
          "aa_length": 258,
          "cds_start": 640,
          "cds_end": null,
          "cds_length": 777,
          "cdna_start": 1117,
          "cdna_end": null,
          "cdna_length": 4116,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "c.388A>G",
          "hgvs_p": "p.Ile130Val",
          "transcript": "ENST00000526349.5",
          "protein_id": "ENSP00000435782.1",
          "transcript_support_level": 1,
          "aa_start": 130,
          "aa_end": null,
          "aa_length": 174,
          "cds_start": 388,
          "cds_end": null,
          "cds_length": 525,
          "cdna_start": 749,
          "cdna_end": null,
          "cdna_length": 1338,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 5,
          "intron_rank_end": null,
          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "c.397-5299A>G",
          "hgvs_p": null,
          "transcript": "ENST00000319104.7",
          "protein_id": "ENSP00000326138.3",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 200,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 603,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1866,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "c.640A>G",
          "hgvs_p": "p.Ile214Val",
          "transcript": "NM_001160223.2",
          "protein_id": "NP_001153695.1",
          "transcript_support_level": null,
          "aa_start": 214,
          "aa_end": null,
          "aa_length": 258,
          "cds_start": 640,
          "cds_end": null,
          "cds_length": 777,
          "cdna_start": 999,
          "cdna_end": null,
          "cdna_length": 3994,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "c.388A>G",
          "hgvs_p": "p.Ile130Val",
          "transcript": "NM_001160225.2",
          "protein_id": "NP_001153697.1",
          "transcript_support_level": null,
          "aa_start": 130,
          "aa_end": null,
          "aa_length": 174,
          "cds_start": 388,
          "cds_end": null,
          "cds_length": 525,
          "cdna_start": 648,
          "cdna_end": null,
          "cdna_length": 3643,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "c.661A>G",
          "hgvs_p": "p.Ile221Val",
          "transcript": "XM_047422278.1",
          "protein_id": "XP_047278234.1",
          "transcript_support_level": null,
          "aa_start": 221,
          "aa_end": null,
          "aa_length": 265,
          "cds_start": 661,
          "cds_end": null,
          "cds_length": 798,
          "cdna_start": 1790,
          "cdna_end": null,
          "cdna_length": 4785,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "c.640A>G",
          "hgvs_p": "p.Ile214Val",
          "transcript": "XM_006716404.3",
          "protein_id": "XP_006716467.1",
          "transcript_support_level": null,
          "aa_start": 214,
          "aa_end": null,
          "aa_length": 258,
          "cds_start": 640,
          "cds_end": null,
          "cds_length": 777,
          "cdna_start": 881,
          "cdna_end": null,
          "cdna_length": 3876,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "c.640A>G",
          "hgvs_p": "p.Ile214Val",
          "transcript": "XM_011544666.4",
          "protein_id": "XP_011542968.1",
          "transcript_support_level": null,
          "aa_start": 214,
          "aa_end": null,
          "aa_length": 258,
          "cds_start": 640,
          "cds_end": null,
          "cds_length": 777,
          "cdna_start": 707,
          "cdna_end": null,
          "cdna_length": 3702,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
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          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 8,
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          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "c.640A>G",
          "hgvs_p": "p.Ile214Val",
          "transcript": "XM_017013881.2",
          "protein_id": "XP_016869370.1",
          "transcript_support_level": null,
          "aa_start": 214,
          "aa_end": null,
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          "cds_start": 640,
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          "cds_length": 777,
          "cdna_start": 1111,
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          "cdna_length": 4106,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "c.640A>G",
          "hgvs_p": "p.Ile214Val",
          "transcript": "XM_047422279.1",
          "protein_id": "XP_047278235.1",
          "transcript_support_level": null,
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          "mane_select": null,
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        },
        {
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          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 5,
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          "exon_count": 5,
          "intron_rank": null,
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          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "c.511A>G",
          "hgvs_p": "p.Ile171Val",
          "transcript": "XM_047422280.1",
          "protein_id": "XP_047278236.1",
          "transcript_support_level": null,
          "aa_start": 171,
          "aa_end": null,
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          "cds_start": 511,
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          "cdna_start": 1647,
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        },
        {
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "c.490A>G",
          "hgvs_p": "p.Ile164Val",
          "transcript": "XM_006716405.4",
          "protein_id": "XP_006716468.1",
          "transcript_support_level": null,
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          "cds_start": 490,
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          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "I",
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          "strand": false,
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            "missense_variant"
          ],
          "exon_rank": 5,
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          "exon_count": 5,
          "intron_rank": null,
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          "gene_symbol": "RNF170",
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          "hgvs_c": "c.490A>G",
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          "transcript": "XM_047422281.1",
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        },
        {
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          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "c.388A>G",
          "hgvs_p": "p.Ile130Val",
          "transcript": "XM_047422282.1",
          "protein_id": "XP_047278238.1",
          "transcript_support_level": null,
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          "cds_start": 388,
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          "cds_length": 525,
          "cdna_start": 528,
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          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "n.*215A>G",
          "hgvs_p": null,
          "transcript": "ENST00000240159.8",
          "protein_id": "ENSP00000240159.4",
          "transcript_support_level": 2,
          "aa_start": null,
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          "cdna_start": null,
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          "cdna_length": 2023,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "n.925A>G",
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          "transcript": "NR_027668.2",
          "protein_id": null,
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        },
        {
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          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "n.695A>G",
          "hgvs_p": null,
          "transcript": "NR_027669.2",
          "protein_id": null,
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          "cdna_length": 3690,
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          "biotype": null,
          "feature": null
        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
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          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "c.*215A>G",
          "hgvs_p": null,
          "transcript": "ENST00000319073.5",
          "protein_id": "ENSP00000325969.5",
          "transcript_support_level": 2,
          "aa_start": null,
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          "aa_length": 116,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 351,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1265,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "n.*215A>G",
          "hgvs_p": null,
          "transcript": "ENST00000240159.8",
          "protein_id": "ENSP00000240159.4",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2023,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 5,
          "intron_rank_end": null,
          "gene_symbol": "RNF170",
          "gene_hgnc_id": 25358,
          "hgvs_c": "c.397-5299A>G",
          "hgvs_p": null,
          "transcript": "NM_001160224.2",
          "protein_id": "NP_001153696.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 200,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 603,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1886,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000286837",
          "gene_hgnc_id": null,
          "hgvs_c": "n.532+12424T>C",
          "hgvs_p": null,
          "transcript": "ENST00000797430.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 663,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "RNF170",
      "gene_hgnc_id": 25358,
      "dbsnp": "rs587780441",
      "frequency_reference_population": 0.00012478918,
      "hom_count_reference_population": 5,
      "allele_count_reference_population": 198,
      "gnomad_exomes_af": 0.000130367,
      "gnomad_genomes_af": 0.000072242,
      "gnomad_exomes_ac": 187,
      "gnomad_genomes_ac": 11,
      "gnomad_exomes_homalt": 5,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.014598697423934937,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.219,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0735,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.34,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 4.496,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PP5,BP4,BS2",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 5,
          "pathogenic_score": 1,
          "criteria": [
            "PP5",
            "BP4",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000527424.6",
          "gene_symbol": "RNF170",
          "hgnc_id": 25358,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.640A>G",
          "hgvs_p": "p.Ile214Val"
        },
        {
          "score": -4,
          "benign_score": 5,
          "pathogenic_score": 1,
          "criteria": [
            "PP5",
            "BP4",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000797430.1",
          "gene_symbol": "ENSG00000286837",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.532+12424T>C",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Autosomal dominant sensory ataxia 1,not specified",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "LP:2 US:2",
      "phenotype_combined": "Autosomal dominant sensory ataxia 1|not specified",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}