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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: X-10449709-T-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=X&pos=10449709&ref=T&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "X",
      "pos": 10449709,
      "ref": "T",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000317552.9",
      "consequences": [
        {
          "aa_ref": "I",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MID1",
          "gene_hgnc_id": 7095,
          "hgvs_c": "c.1663A>C",
          "hgvs_p": "p.Ile555Leu",
          "transcript": "NM_000381.4",
          "protein_id": "NP_000372.1",
          "transcript_support_level": null,
          "aa_start": 555,
          "aa_end": null,
          "aa_length": 667,
          "cds_start": 1663,
          "cds_end": null,
          "cds_length": 2004,
          "cdna_start": 2015,
          "cdna_end": null,
          "cdna_length": 6168,
          "mane_select": "ENST00000317552.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MID1",
          "gene_hgnc_id": 7095,
          "hgvs_c": "c.1663A>C",
          "hgvs_p": "p.Ile555Leu",
          "transcript": "ENST00000317552.9",
          "protein_id": "ENSP00000312678.4",
          "transcript_support_level": 1,
          "aa_start": 555,
          "aa_end": null,
          "aa_length": 667,
          "cds_start": 1663,
          "cds_end": null,
          "cds_length": 2004,
          "cdna_start": 2015,
          "cdna_end": null,
          "cdna_length": 6168,
          "mane_select": "NM_000381.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MID1",
          "gene_hgnc_id": 7095,
          "hgvs_c": "c.1663A>C",
          "hgvs_p": "p.Ile555Leu",
          "transcript": "ENST00000380779.5",
          "protein_id": "ENSP00000370156.1",
          "transcript_support_level": 1,
          "aa_start": 555,
          "aa_end": null,
          "aa_length": 667,
          "cds_start": 1663,
          "cds_end": null,
          "cds_length": 2004,
          "cdna_start": 1891,
          "cdna_end": null,
          "cdna_length": 6044,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MID1",
          "gene_hgnc_id": 7095,
          "hgvs_c": "c.1663A>C",
          "hgvs_p": "p.Ile555Leu",
          "transcript": "ENST00000380780.5",
          "protein_id": "ENSP00000370157.1",
          "transcript_support_level": 1,
          "aa_start": 555,
          "aa_end": null,
          "aa_length": 667,
          "cds_start": 1663,
          "cds_end": null,
          "cds_length": 2004,
          "cdna_start": 2042,
          "cdna_end": null,
          "cdna_length": 6195,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MID1",
          "gene_hgnc_id": 7095,
          "hgvs_c": "c.1663A>C",
          "hgvs_p": "p.Ile555Leu",
          "transcript": "ENST00000380787.5",
          "protein_id": "ENSP00000370164.1",
          "transcript_support_level": 1,
          "aa_start": 555,
          "aa_end": null,
          "aa_length": 667,
          "cds_start": 1663,
          "cds_end": null,
          "cds_length": 2004,
          "cdna_start": 1820,
          "cdna_end": null,
          "cdna_length": 5973,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": 9,
          "intron_rank_end": null,
          "gene_symbol": "MID1",
          "gene_hgnc_id": 7095,
          "hgvs_c": "c.1656-93A>C",
          "hgvs_p": null,
          "transcript": "ENST00000380782.6",
          "protein_id": "ENSP00000370159.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 552,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 1659,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3352,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MID1",
          "gene_hgnc_id": 7095,
          "hgvs_c": "c.1816A>C",
          "hgvs_p": "p.Ile606Leu",
          "transcript": "ENST00000675073.1",
          "protein_id": "ENSP00000501707.1",
          "transcript_support_level": null,
          "aa_start": 606,
          "aa_end": null,
          "aa_length": 718,
          "cds_start": 1816,
          "cds_end": null,
          "cds_length": 2157,
          "cdna_start": 1872,
          "cdna_end": null,
          "cdna_length": 2369,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MID1",
          "gene_hgnc_id": 7095,
          "hgvs_c": "c.1663A>C",
          "hgvs_p": "p.Ile555Leu",
          "transcript": "NM_001098624.2",
          "protein_id": "NP_001092094.1",
          "transcript_support_level": null,
          "aa_start": 555,
          "aa_end": null,
          "aa_length": 667,
          "cds_start": 1663,
          "cds_end": null,
          "cds_length": 2004,
          "cdna_start": 1994,
          "cdna_end": null,
          "cdna_length": 6393,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MID1",
          "gene_hgnc_id": 7095,
          "hgvs_c": "c.1663A>C",
          "hgvs_p": "p.Ile555Leu",
          "transcript": "NM_001193277.1",
          "protein_id": "NP_001180206.1",
          "transcript_support_level": null,
          "aa_start": 555,
          "aa_end": null,
          "aa_length": 667,
          "cds_start": 1663,
          "cds_end": null,
          "cds_length": 2004,
          "cdna_start": 1853,
          "cdna_end": null,
          "cdna_length": 6252,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MID1",
          "gene_hgnc_id": 7095,
          "hgvs_c": "c.1663A>C",
          "hgvs_p": "p.Ile555Leu",
          "transcript": "NM_001347733.2",
          "protein_id": "NP_001334662.1",
          "transcript_support_level": null,
          "aa_start": 555,
          "aa_end": null,
          "aa_length": 667,
          "cds_start": 1663,
          "cds_end": null,
          "cds_length": 2004,
          "cdna_start": 2093,
          "cdna_end": null,
          "cdna_length": 6492,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
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          "gene_symbol": "MID1",
          "gene_hgnc_id": 7095,
          "hgvs_c": "c.1663A>C",
          "hgvs_p": "p.Ile555Leu",
          "transcript": "NM_033290.4",
          "protein_id": "NP_150632.1",
          "transcript_support_level": null,
          "aa_start": 555,
          "aa_end": null,
          "aa_length": 667,
          "cds_start": 1663,
          "cds_end": null,
          "cds_length": 2004,
          "cdna_start": 1849,
          "cdna_end": null,
          "cdna_length": 6248,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MID1",
          "gene_hgnc_id": 7095,
          "hgvs_c": "c.1663A>C",
          "hgvs_p": "p.Ile555Leu",
          "transcript": "ENST00000380785.5",
          "protein_id": "ENSP00000370162.1",
          "transcript_support_level": 2,
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          "cds_start": 1663,
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          "cdna_start": 1939,
          "cdna_end": null,
          "cdna_length": 6092,
          "mane_select": null,
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        {
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          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 10,
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          "exon_count": 10,
          "intron_rank": null,
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          "gene_symbol": "MID1",
          "gene_hgnc_id": 7095,
          "hgvs_c": "c.1663A>C",
          "hgvs_p": "p.Ile555Leu",
          "transcript": "ENST00000413894.6",
          "protein_id": "ENSP00000391154.2",
          "transcript_support_level": 5,
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          "cds_start": 1663,
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          "cdna_start": 1942,
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        },
        {
          "aa_ref": "I",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MID1",
          "gene_hgnc_id": 7095,
          "hgvs_c": "c.1663A>C",
          "hgvs_p": "p.Ile555Leu",
          "transcript": "ENST00000453318.6",
          "protein_id": "ENSP00000414521.2",
          "transcript_support_level": 5,
          "aa_start": 555,
          "aa_end": null,
          "aa_length": 667,
          "cds_start": 1663,
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          "cdna_start": 1994,
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          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "I",
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          "strand": false,
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          ],
          "exon_rank": 9,
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          "intron_rank": null,
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          "gene_symbol": "MID1",
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          "hgvs_c": "c.1555A>C",
          "hgvs_p": "p.Ile519Leu",
          "transcript": "ENST00000691943.1",
          "protein_id": "ENSP00000508663.1",
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          "cds_start": 1555,
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        {
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
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          "gene_symbol": "MID1",
          "gene_hgnc_id": 7095,
          "hgvs_c": "c.1549A>C",
          "hgvs_p": "p.Ile517Leu",
          "transcript": "NM_033289.2",
          "protein_id": "NP_150631.1",
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          "aa_length": 629,
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          "cdna_start": 1901,
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        {
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          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 10,
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          "exon_count": 10,
          "intron_rank": null,
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          "gene_symbol": "MID1",
          "gene_hgnc_id": 7095,
          "hgvs_c": "c.1549A>C",
          "hgvs_p": "p.Ile517Leu",
          "transcript": "ENST00000689773.1",
          "protein_id": "ENSP00000509925.1",
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          "cdna_start": 1880,
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        },
        {
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          "gene_symbol": "MID1",
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          "hgvs_c": "c.1549A>C",
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          "transcript": "ENST00000690004.1",
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        },
        {
          "aa_ref": "I",
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          "protein_coding": true,
          "strand": false,
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          ],
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          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MID1",
          "gene_hgnc_id": 7095,
          "hgvs_c": "c.796A>C",
          "hgvs_p": "p.Ile266Leu",
          "transcript": "ENST00000674917.1",
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          "cdna_start": 1046,
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          "feature": null
        },
        {
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          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MID1",
          "gene_hgnc_id": 7095,
          "hgvs_c": "n.613A>C",
          "hgvs_p": null,
          "transcript": "ENST00000479925.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 858,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "MID1",
      "gene_hgnc_id": 7095,
      "dbsnp": "rs398123341",
      "frequency_reference_population": 0.00006250886,
      "hom_count_reference_population": 22,
      "allele_count_reference_population": 75,
      "gnomad_exomes_af": 0.0000652676,
      "gnomad_genomes_af": 0.0000357143,
      "gnomad_exomes_ac": 71,
      "gnomad_genomes_ac": 4,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.30959078669548035,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.138,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1438,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.42,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 3.331,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -10,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4,BP6,BS1,BS2",
      "acmg_by_gene": [
        {
          "score": -10,
          "benign_score": 10,
          "pathogenic_score": 0,
          "criteria": [
            "BP4",
            "BP6",
            "BS1",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000317552.9",
          "gene_symbol": "MID1",
          "hgnc_id": 7095,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "XL",
          "hgvs_c": "c.1663A>C",
          "hgvs_p": "p.Ile555Leu"
        }
      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:1 B:1",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}