NM_000914.5:c.1164+648A>G

Variant names:

• chr6-154092120-A-G
• rs10485057
• NM_000914.5:c.1164+648A>G

Variant summary

Our verdict is Benign. The variant received -12 ACMG points: 0P and 12B. BP4_Strong BA1

The NM_000914.5(OPRM1):​c.1164+648A>G variant causes a intron change involving the alteration of a non-conserved nucleotide. The variant allele was found at a frequency of 0.106 in 152,162 control chromosomes in the GnomAD database, including 955 homozygotes. In-silico tool predicts a benign outcome for this variant. No clinical diagnostic laboratories have submitted clinical-significance assessments for this variant to ClinVar.

• Frequency: Genomes: 𝑓 0.11 (955 hom., cov: 32)
• Consequence: OPRM1 NM_000914.5 intron
• Clinical Significance: Not reported in ClinVar
• Conservation: PhyloP100: -0.0300
• Publications: 14 publications found

Genes affected

OPRM1 (HGNC:8156): (opioid receptor mu 1) This gene encodes one of at least three opioid receptors in humans; the mu opioid receptor (MOR). The MOR is the principal target of endogenous opioid peptides and opioid analgesic agents such as beta-endorphin and enkephalins. The MOR also has an important role in dependence to other drugs of abuse, such as nicotine, cocaine, and alcohol via its modulation of the dopamine system. The NM_001008503.2:c.118A>G allele has been associated with opioid and alcohol addiction and variations in pain sensitivity but evidence for it having a causal role is conflicting. Multiple transcript variants encoding different isoforms have been found for this gene. Though the canonical MOR belongs to the superfamily of 7-transmembrane-spanning G-protein-coupled receptors some isoforms of this gene have only 6 transmembrane domains. [provided by RefSeq, Oct 2013]

ACMG classification

Our verdict: Benign. The variant received -12 ACMG points.

• BP4: Computational evidence support a benign effect (BayesDel_noAF=-0.79).
• BA1: GnomAd4 highest subpopulation (AFR) allele frequency at 95% confidence interval = 0.146 is higher than 0.05.

Variant Effect in Transcripts

ACMG analysis was done for transcript: NM_000914.5. You can select a different transcript below to see updated ACMG assignments.

RefSeq Transcripts

Selected	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	OPRM1	NM_000914.5	MANE Select	c.1164+648A>G		intron	N/A	NP_000905.3
	OPRM1	NM_001145279.4		c.1443+648A>G		intron	N/A	NP_001138751.1
	OPRM1	NM_001285524.1		c.1443+648A>G		intron	N/A	NP_001272453.1

Ensembl Transcripts

Selected	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	OPRM1	ENST00000330432.12	TSL:1 MANE Select	c.1164+648A>G		intron	N/A	ENSP00000328264.7
	OPRM1	ENST00000434900.6	TSL:1	c.1443+648A>G		intron	N/A	ENSP00000394624.2
	OPRM1	ENST00000229768.9	TSL:1	c.1164+648A>G		intron	N/A	ENSP00000229768.5

Frequencies

GnomAD3 genomes AF: 0.106 AC: 16086 AN: 152048 Hom.: 950 Cov.: 32

Gnomad AFR AF: 0.148

Gnomad AMI AF: 0.128

Gnomad AMR AF: 0.0755

Gnomad ASJ AF: 0.118

Gnomad EAS AF: 0.0423

Gnomad SAS AF: 0.0251

Gnomad FIN AF: 0.124

Gnomad MID AF: 0.0573

Gnomad NFE AF: 0.0942

Gnomad OTH AF: 0.0937

We have no GnomAD4 exomes data on this position. Probably position not covered by the project.

GnomAD4 genome AF: 0.106 AC: 16124 AN: 152162 Hom.: 955 Cov.: 32 AF XY: 0.105 AC XY: 7827 AN XY: 74388

African (AFR) AF: 0.149 AC: 6176 AN: 41516

American (AMR) AF: 0.0754 AC: 1153 AN: 15294

Ashkenazi Jewish (ASJ) AF: 0.118 AC: 410 AN: 3472

East Asian (EAS) AF: 0.0426 AC: 221 AN: 5186

South Asian (SAS) AF: 0.0251 AC: 121 AN: 4824

European-Finnish (FIN) AF: 0.124 AC: 1305 AN: 10558

Middle Eastern (MID) AF: 0.0582 AC: 17 AN: 292

European-Non Finnish (NFE) AF: 0.0942 AC: 6408 AN: 67998

Other (OTH) AF: 0.0929 AC: 196 AN: 2110

Alfa AF: 0.0982 Hom.: 280

Bravo AF: 0.104

Asia WGS AF: 0.0360 AC: 125 AN: 3468

ClinVar

Not reported in ClinVar

Computational scores

Source: dbNSFP v4.9

Name	Calibrated prediction	Score	Prediction
BayesDel_noAF	Benign	-0.79
CADD	Benign	3.5
DANN	Benign	0.85
PhyloP100		-0.030
Mutation Taster		=100/0	polymorphism (auto)

Splicing

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Other links and lift over

dbSNP: rs10485057; hg19: chr6-154413255;