NM_001136263.2:c.890G>A

Variant summary

Our verdict is Likely benign. Variant got -2 ACMG points: 2P and 4B. PM2BP4_Strong

The NM_001136263.2(C2CD4C):​c.890G>A​(p.Gly297Glu) variant causes a missense change involving the alteration of a non-conserved nucleotide. The variant allele was found at a frequency of 0.0000114 in 1,400,282 control chromosomes in the GnomAD database, with no homozygous occurrence. In-silico tool predicts a benign outcome for this variant. 14/20 in silico tools predict a benign outcome for this variant. Variant has been reported in ClinVar as Uncertain significance (★).

Frequency

Genomes: 𝑓 0.000013 ( 0 hom., cov: 33)
Exomes 𝑓: 0.000011 ( 0 hom. )

Consequence

C2CD4C
NM_001136263.2 missense

Scores

1
18

Clinical Significance

Uncertain significance criteria provided, single submitter U:1

Conservation

PhyloP100: 0.291
Variant links:
Genes affected
C2CD4C (HGNC:29417): (C2 calcium dependent domain containing 4C) Predicted to be active in cytosol. [provided by Alliance of Genome Resources, Apr 2022]

Genome browser will be placed here

ACMG classification

Classification made for transcript

Verdict is Likely_benign. Variant got -2 ACMG points.

PM2
Very rare variant in population databases, with high coverage;
BP4
Computational evidence support a benign effect (MetaRNN=0.04661587).

Transcripts

RefSeq

Gene Transcript HGVSc HGVSp Effect Exon rank MANE Protein UniProt
C2CD4CNM_001136263.2 linkc.890G>A p.Gly297Glu missense_variant Exon 2 of 2 ENST00000332235.8 NP_001129735.1 Q8TF44
C2CD4CXM_011527694.2 linkc.890G>A p.Gly297Glu missense_variant Exon 2 of 3 XP_011525996.1

Ensembl

Gene Transcript HGVSc HGVSp Effect Exon rank TSL MANE Protein Appris UniProt
C2CD4CENST00000332235.8 linkc.890G>A p.Gly297Glu missense_variant Exon 2 of 2 2 NM_001136263.2 ENSP00000328677.4 Q8TF44

Frequencies

GnomAD3 genomes
AF:
0.0000132
AC:
2
AN:
151970
Hom.:
0
Cov.:
33
show subpopulations
Gnomad AFR
AF:
0.00
Gnomad AMI
AF:
0.00
Gnomad AMR
AF:
0.00
Gnomad ASJ
AF:
0.00
Gnomad EAS
AF:
0.00
Gnomad SAS
AF:
0.00
Gnomad FIN
AF:
0.00
Gnomad MID
AF:
0.00
Gnomad NFE
AF:
0.0000294
Gnomad OTH
AF:
0.00
GnomAD3 exomes
AF:
0.0000402
AC:
1
AN:
24878
Hom.:
0
AF XY:
0.00
AC XY:
0
AN XY:
12950
show subpopulations
Gnomad AFR exome
AF:
0.00
Gnomad AMR exome
AF:
0.00
Gnomad ASJ exome
AF:
0.00
Gnomad EAS exome
AF:
0.00
Gnomad SAS exome
AF:
0.00
Gnomad FIN exome
AF:
0.00
Gnomad NFE exome
AF:
0.0000966
Gnomad OTH exome
AF:
0.00
GnomAD4 exome
AF:
0.0000112
AC:
14
AN:
1248312
Hom.:
0
Cov.:
31
AF XY:
0.0000132
AC XY:
8
AN XY:
605416
show subpopulations
Gnomad4 AFR exome
AF:
0.00
Gnomad4 AMR exome
AF:
0.00
Gnomad4 ASJ exome
AF:
0.00
Gnomad4 EAS exome
AF:
0.00
Gnomad4 SAS exome
AF:
0.00
Gnomad4 FIN exome
AF:
0.00
Gnomad4 NFE exome
AF:
0.0000138
Gnomad4 OTH exome
AF:
0.00
GnomAD4 genome
AF:
0.0000132
AC:
2
AN:
151970
Hom.:
0
Cov.:
33
AF XY:
0.0000135
AC XY:
1
AN XY:
74234
show subpopulations
Gnomad4 AFR
AF:
0.00
Gnomad4 AMR
AF:
0.00
Gnomad4 ASJ
AF:
0.00
Gnomad4 EAS
AF:
0.00
Gnomad4 SAS
AF:
0.00
Gnomad4 FIN
AF:
0.00
Gnomad4 NFE
AF:
0.0000294
Gnomad4 OTH
AF:
0.00
Bravo
AF:
0.0000189

ClinVar

Significance: Uncertain significance
Submissions summary: Uncertain:1
Revision: criteria provided, single submitter
LINK: link

Submissions by phenotype

not specified Uncertain:1
Jan 29, 2024
Ambry Genetics
Significance: Uncertain significance
Review Status: criteria provided, single submitter
Collection Method: clinical testing

The c.890G>A (p.G297E) alteration is located in exon 2 (coding exon 1) of the C2CD4C gene. This alteration results from a G to A substitution at nucleotide position 890, causing the glycine (G) at amino acid position 297 to be replaced by a glutamic acid (E). Based on insufficient or conflicting evidence, the clinical significance of this alteration remains unclear. -

Computational scores

Source: dbNSFP v4.3

Name
Calibrated prediction
Score
Prediction
AlphaMissense
Benign
0.095
BayesDel_addAF
Benign
-0.49
T
BayesDel_noAF
Benign
-0.73
CADD
Benign
11
DANN
Benign
0.84
DEOGEN2
Benign
0.026
T
Eigen
Benign
-1.2
Eigen_PC
Benign
-1.2
FATHMM_MKL
Benign
0.035
N
LIST_S2
Benign
0.40
T
M_CAP
Benign
0.018
T
MetaRNN
Benign
0.047
T
MetaSVM
Benign
-0.98
T
MutationAssessor
Benign
1.2
L
PrimateAI
Pathogenic
0.92
D
PROVEAN
Benign
-1.4
N
REVEL
Benign
0.016
Sift
Benign
0.66
T
Sift4G
Benign
0.40
T
Polyphen
0.0020
B
Vest4
0.19
MutPred
0.21
Gain of solvent accessibility (P = 0.012);
MVP
0.061
ClinPred
0.018
T
GERP RS
0.076
Varity_R
0.034
gMVP
0.33

Splicing

Name
Calibrated prediction
Score
Prediction
SpliceAI score (max)
0.0
Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Publications

LitVar

Below is the list of publications found by LitVar. It may be empty.

Other links and lift over

dbSNP: rs1444280271; hg19: chr19-407472; API