NM_001387777.1:c.3901T>A

Variant names:

• chr2-217818431-A-T
• rs2571445
• NM_001387777.1:c.3901T>A

Variant summary

Our verdict is Likely benign. The variant received -2 ACMG points: 2P and 4B. PM2 BP4_Strong

The NM_001387777.1(TNS1):​c.3901T>A​(p.Trp1301Arg) variant causes a missense change involving the alteration of a non-conserved nucleotide. The variant allele was found at a frequency of 0.000000684 in 1,461,692 control chromosomes in the GnomAD database, with no homozygous occurrence. In-silico tool predicts a benign outcome for this variant. 16/22 in silico tools predict a benign outcome for this variant. Variant has been reported in ClinVar as (no stars). Another nucleotide change resulting in the same amino acid substitution has been previously reported as Benign in ClinVar.

• Frequency:
  • Genomes: not found (cov: 34)
  • Exomes 𝑓: 6.8e-7 (0 hom.)
• Consequence: TNS1 NM_001387777.1 missense
• Clinical Significance: Not reported in ClinVar
• Conservation: PhyloP100: 2.70
• Publications: 113 publications found

Genes affected

TNS1 (HGNC:11973): (tensin 1) The protein encoded by this gene localizes to focal adhesions, regions of the plasma membrane where the cell attaches to the extracellular matrix. This protein crosslinks actin filaments and contains a Src homology 2 (SH2) domain, which is often found in molecules involved in signal transduction. This protein is a substrate of calpain II. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

ACMG classification

Our verdict: Likely_benign. The variant received -2 ACMG points.

• PM2: Very rare variant in population databases, with high coverage
• BP4: Computational evidence support a benign effect (MetaRNN=0.029339314).

Variant Effect in Transcripts

ACMG analysis was done for transcript: NM_001387777.1. You can select a different transcript below to see updated ACMG assignments.

RefSeq Transcripts

Sel.	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	TNS1	NM_001387777.1	MANE Select	c.3901T>A	p.Trp1301Arg	missense	Exon 24 of 33	NP_001374706.1	Q9HBL0-3
	TNS1	NM_001438865.1		c.3964T>A	p.Trp1322Arg	missense	Exon 24 of 33	NP_001425794.1
	TNS1	NM_001438866.1		c.3901T>A	p.Trp1301Arg	missense	Exon 24 of 33	NP_001425795.1

Ensembl Transcripts

Sel.	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	TNS1	ENST00000682258.1	MANE Select	c.3901T>A	p.Trp1301Arg	missense	Exon 24 of 33	ENSP00000506917.1	Q9HBL0-3
	TNS1	ENST00000171887.8	TSL:1	c.3589T>A	p.Trp1197Arg	missense	Exon 24 of 33	ENSP00000171887.4	Q9HBL0-1
	TNS1	ENST00000419504.6	TSL:1	c.3550T>A	p.Trp1184Arg	missense	Exon 23 of 32	ENSP00000408724.1	E9PF55

Frequencies

GnomAD3 genomes Cov.: 34

GnomAD4 exome AF: 6.84e-7 AC: 1 AN: 1461692 Hom.: 0 Cov.: 91 AF XY: 0.00 AC XY: 0 AN XY: 727112

African (AFR) AF: 0.00 AC: 0 AN: 33474

American (AMR) AF: 0.00 AC: 0 AN: 44714

Ashkenazi Jewish (ASJ) AF: 0.00 AC: 0 AN: 26132

East Asian (EAS) AF: 0.00 AC: 0 AN: 39698

South Asian (SAS) AF: 0.00 AC: 0 AN: 86240

European-Finnish (FIN) AF: 0.00 AC: 0 AN: 53368

Middle Eastern (MID) AF: 0.00 AC: 0 AN: 5762

European-Non Finnish (NFE) AF: 8.99e-7 AC: 1 AN: 1111918

Other (OTH) AF: 0.00 AC: 0 AN: 60386

GnomAD4 genome Cov.: 34

Alfa AF: 0.00 Hom.: 98412

ClinVar

Not reported in ClinVar

Computational scores

Source: dbNSFP v4.9

Name	Calibrated prediction	Score	Prediction
AlphaMissense	Benign	0.048
BayesDel_addAF	Benign	-0.12	T
BayesDel_noAF	Benign	-0.41
CADD	Benign	15
DANN	Benign	0.67
DEOGEN2	Benign	0.033	T
Eigen	Benign	-0.92
Eigen_PC	Benign	-0.68
FATHMM_MKL	Benign	0.61	D
LIST_S2	Benign	0.082	T
M_CAP	Benign	0.068	D
MetaRNN	Benign	0.029	T
MetaSVM	Benign	-0.75	T
MutationAssessor	Benign	-2.2	N
PhyloP100		2.7
PrimateAI	Uncertain	0.54	T
PROVEAN	Benign	3.8	N
REVEL	Benign	0.15
Sift	Benign	1.0	T
Sift4G	Benign	0.52	T
Polyphen		0.0	B
Vest4		0.18
MutPred		0.18		Gain of methylation at W1197 (P = 0.0296)
MVP		0.082
MPC		0.22
ClinPred		0.039	T
GERP RS		3.7
Varity_R		0.072
gMVP		0.33
Mutation Taster		=100/0	polymorphism

Splicing

Name	Calibrated prediction	Score	Prediction
SpliceAI score (max)		0.0		Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Other links and lift over

dbSNP: rs2571445; hg19: chr2-218683154;