NM_006522.4:c.81-3607C>G

Variant names:

• chr2-218867420-C-G
• rs6754599
• NM_006522.4:c.81-3607C>G

Variant summary

Our verdict is Benign. The variant received -12 ACMG points: 0P and 12B. BP4_Strong BA1

The NM_006522.4(WNT6):​c.81-3607C>G variant causes a intron change involving the alteration of a non-conserved nucleotide. The variant allele was found at a frequency of 0.35 in 152,060 control chromosomes in the GnomAD database, including 14,755 homozygotes. In-silico tool predicts a benign outcome for this variant. No clinical diagnostic laboratories have submitted clinical-significance assessments for this variant to ClinVar.

• Frequency: Genomes: 𝑓 0.35 (14755 hom., cov: 32)
• Consequence: WNT6 NM_006522.4 intron
• Clinical Significance: Not reported in ClinVar
• Conservation: PhyloP100: 0.775
• Publications: 11 publications found

Genes affected

WNT6 (HGNC:12785): (Wnt family member 6) The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is overexpressed in cervical cancer cell line and strongly coexpressed with another family member, WNT10A, in colorectal cancer cell line. The gene overexpression may play key roles in carcinogenesis. This gene and the WNT10A gene are clustered in the chromosome 2q35 region. The protein encoded by this gene is 97% identical to the mouse Wnt6 protein at the amino acid level. [provided by RefSeq, Jul 2008]

ENSG00000308857 (HGNC:):

ACMG classification

Our verdict: Benign. The variant received -12 ACMG points.

• BP4: Computational evidence support a benign effect (BayesDel_noAF=-0.73).
• BA1: GnomAd4 highest subpopulation (AFR) allele frequency at 95% confidence interval = 0.761 is higher than 0.05.

Variant Effect in Transcripts

ACMG analysis was done for transcript: NM_006522.4. You can select a different transcript below to see updated ACMG assignments.

RefSeq Transcripts

Selected	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	WNT6	NM_006522.4	MANE Select	c.81-3607C>G		intron	N/A	NP_006513.1

Ensembl Transcripts

Selected	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	WNT6	ENST00000233948.4	TSL:1 MANE Select	c.81-3607C>G		intron	N/A	ENSP00000233948.3
	WNT6	ENST00000486233.1	TSL:5	n.154-4065C>G		intron	N/A
	ENSG00000308857	ENST00000836898.1		n.97-562G>C		intron	N/A

Frequencies

GnomAD3 genomes AF: 0.349 AC: 53095 AN: 151942 Hom.: 14708 Cov.: 32

Gnomad AFR AF: 0.767

Gnomad AMI AF: 0.132

Gnomad AMR AF: 0.277

Gnomad ASJ AF: 0.159

Gnomad EAS AF: 0.395

Gnomad SAS AF: 0.282

Gnomad FIN AF: 0.132

Gnomad MID AF: 0.272

Gnomad NFE AF: 0.161

Gnomad OTH AF: 0.305

We have no GnomAD4 exomes data on this position. Probably position not covered by the project.

GnomAD4 genome AF: 0.350 AC: 53196 AN: 152060 Hom.: 14755 Cov.: 32 AF XY: 0.345 AC XY: 25662 AN XY: 74350

African (AFR) AF: 0.768 AC: 31817 AN: 41446

American (AMR) AF: 0.277 AC: 4227 AN: 15278

Ashkenazi Jewish (ASJ) AF: 0.159 AC: 551 AN: 3468

East Asian (EAS) AF: 0.394 AC: 2037 AN: 5166

South Asian (SAS) AF: 0.280 AC: 1350 AN: 4814

European-Finnish (FIN) AF: 0.132 AC: 1402 AN: 10602

Middle Eastern (MID) AF: 0.262 AC: 77 AN: 294

European-Non Finnish (NFE) AF: 0.161 AC: 10960 AN: 67970

Other (OTH) AF: 0.310 AC: 655 AN: 2112

Alfa AF: 0.251 Hom.: 1138

Bravo AF: 0.376

Asia WGS AF: 0.411 AC: 1428 AN: 3478

ClinVar

Not reported in ClinVar

Computational scores

Source: dbNSFP v4.9

Name	Calibrated prediction	Score	Prediction
BayesDel_noAF	Benign	-0.73
CADD	Benign	12
DANN	Benign	0.84
PhyloP100		0.78
Mutation Taster		=100/0	polymorphism (auto)

Splicing

Name	Calibrated prediction	Score	Prediction
SpliceAI score (max)		0.0		Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Other links and lift over

dbSNP: rs6754599; hg19: chr2-219732142;