NM_022093.2:c.3331-262T>C

Variant names:

• chr1-175135583-T-C
• rs4651322
• NM_022093.2:c.3331-262T>C

Variant summary

Our verdict is Benign. The variant received -12 ACMG points: 0P and 12B. BP4_Strong BA1

The NM_022093.2(TNN):​c.3331-262T>C variant causes a intron change involving the alteration of a non-conserved nucleotide. The variant allele was found at a frequency of 0.615 in 152,158 control chromosomes in the GnomAD database, including 28,874 homozygotes. In-silico tool predicts a benign outcome for this variant. No clinical diagnostic laboratories have submitted clinical-significance assessments for this variant to ClinVar.

• Frequency: Genomes: 𝑓 0.61 (28874 hom., cov: 33)
• Consequence: TNN NM_022093.2 intron
• Clinical Significance: Not reported in ClinVar
• Conservation: PhyloP100: -0.735
• Publications: 2 publications found

Genes affected

TNN (HGNC:22942): (tenascin N) Predicted to enable integrin binding activity. Predicted to be involved in several processes, including generation of neurons; negative regulation of canonical Wnt signaling pathway involved in osteoblast differentiation; and negative regulation of osteoblast differentiation. Predicted to act upstream of or within axonogenesis. Predicted to be located in extracellular matrix and neuron projection. Predicted to be active in collagen-containing extracellular matrix and extracellular space. [provided by Alliance of Genome Resources, Apr 2022]

ACMG classification

Our verdict: Benign. The variant received -12 ACMG points.

• BP4: Computational evidence support a benign effect (BayesDel_noAF=-0.93).
• BA1: GnomAd4 highest subpopulation (AMR) allele frequency at 95% confidence interval = 0.635 is higher than 0.05.

Variant Effect in Transcripts

ACMG analysis was done for transcript: NM_022093.2. You can select a different transcript below to see updated ACMG assignments.

RefSeq Transcripts

Selected	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	TNN	NM_022093.2	MANE Select	c.3331-262T>C		intron	N/A	NP_071376.1

Ensembl Transcripts

Selected	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	TNN	ENST00000239462.9	TSL:2 MANE Select	c.3331-262T>C		intron	N/A	ENSP00000239462.4
	TNN	ENST00000621086.1	TSL:5	c.2800-262T>C		intron	N/A	ENSP00000480895.1

Frequencies

GnomAD3 genomes AF: 0.615 AC: 93469 AN: 152040 Hom.: 28844 Cov.: 33

Gnomad AFR AF: 0.601

Gnomad AMI AF: 0.616

Gnomad AMR AF: 0.645

Gnomad ASJ AF: 0.547

Gnomad EAS AF: 0.523

Gnomad SAS AF: 0.543

Gnomad FIN AF: 0.622

Gnomad MID AF: 0.604

Gnomad NFE AF: 0.631

Gnomad OTH AF: 0.612

We have no GnomAD4 exomes data on this position. Probably position not covered by the project.

GnomAD4 genome AF: 0.615 AC: 93549 AN: 152158 Hom.: 28874 Cov.: 33 AF XY: 0.616 AC XY: 45830 AN XY: 74380

African (AFR) AF: 0.601 AC: 24966 AN: 41520

American (AMR) AF: 0.646 AC: 9862 AN: 15278

Ashkenazi Jewish (ASJ) AF: 0.547 AC: 1895 AN: 3466

East Asian (EAS) AF: 0.523 AC: 2708 AN: 5180

South Asian (SAS) AF: 0.542 AC: 2620 AN: 4830

European-Finnish (FIN) AF: 0.622 AC: 6578 AN: 10570

Middle Eastern (MID) AF: 0.609 AC: 179 AN: 294

European-Non Finnish (NFE) AF: 0.631 AC: 42890 AN: 68002

Other (OTH) AF: 0.612 AC: 1290 AN: 2108

Alfa AF: 0.621 Hom.: 15057

Bravo AF: 0.614

Asia WGS AF: 0.583 AC: 2024 AN: 3478

ClinVar

Not reported in ClinVar

Computational scores

Source: dbNSFP v4.9

Name	Calibrated prediction	Score	Prediction
BayesDel_noAF	Benign	-0.93
CADD	Benign	2.4
DANN	Benign	0.49
PhyloP100		-0.73
Mutation Taster		=100/0	polymorphism (auto)

Splicing

Name	Calibrated prediction	Score	Prediction
SpliceAI score (max)		0.0		Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Other links and lift over

dbSNP: rs4651322; hg19: chr1-175104719;