Genetic Variant FZD4:p.Pro168Ser (chr11-86952254-G-A) – ACMG Classification: Benign (-20 pts)

Variant summary

Our verdict is Benign. The variant received -20 ACMG points: 0P and 20B. BP4_StrongBP6_Very_StrongBS1BS2

The NM_012193.4(FZD4):c.502C>T(p.Pro168Ser) variant causes a missense change. The variant allele was found at a frequency of 0.0184 in 1,614,150 control chromosomes in the GnomAD database, including 353 homozygotes. In-silico tool predicts a benign outcome for this variant. Variant has been reported in ClinVar as Benign (★★).

Frequency

Genomes: 𝑓 0.014 ( 22 hom., cov: 33)

Exomes 𝑓: 0.019 ( 331 hom. )

Consequence

FZD4
NM_012193.4 missense

Scores

Clinical Significance

Benign criteria provided, multiple submitters, no conflicts B:5

Conservation

PhyloP100: 6.52

Publications

25 publications found

Variant links:

COSMIC-nc: COSV107540118

Genes affected

FZD4 (HGNC:4042): (frizzled class receptor 4) This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence. [provided by RefSeq, Jul 2008]

FZD4 links:

PRSS23 (HGNC:14370): (serine protease 23) This gene encodes a conserved member of the trypsin family of serine proteases. Mouse studies found a decrease of mRNA levels of this gene after ovulation was induced. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

PRSS23 links:

Genome browser will be placed here

ACMG classification

Classification was made for transcript

Our verdict: Benign. The variant received -20 ACMG points.

BP4

Computational evidence support a benign effect (MetaRNN=0.0032744408).

BP6

Variant 11-86952254-G-A is Benign according to our data. Variant chr11-86952254-G-A is described in ClinVar as Benign. ClinVar VariationId is 306407.Status of the report is criteria_provided_multiple_submitters_no_conflicts, 2 stars.

BS1

Variant frequency is greater than expected in population sas. GnomAd4 allele frequency = 0.0144 (2186/152294) while in subpopulation SAS AF = 0.0261 (126/4828). AF 95% confidence interval is 0.0224. There are 22 homozygotes in GnomAd4. There are 1040 alleles in the male GnomAd4 subpopulation. Median coverage is 33. This position passed quality control check.

BS2

High AC in GnomAd4 at 2186 SD,AD,Unknown gene.

Variant Effect in Transcripts

ACMG analysis was done for transcript: NM_012193.4. You can select a different transcript below to see updated ACMG assignments.

RefSeq Transcripts

Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein
FZD4	NM_012193.4	MANE Select	c.502C>T	p.Pro168Ser	missense	Exon 2 of 2	NP_036325.2
PRSS23	NR_120591.3		n.1617G>A		non_coding_transcript_exon	Exon 5 of 5
PRSS23	NR_120592.2		n.1366G>A		non_coding_transcript_exon	Exon 3 of 3

Ensembl Transcripts

Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
FZD4	ENST00000531380.2	TSL:1 MANE Select	c.502C>T	p.Pro168Ser	missense	Exon 2 of 2	ENSP00000434034.1	Q9ULV1
PRSS23	ENST00000532234.5	TSL:1	n.*1247G>A		non_coding_transcript_exon	Exon 5 of 5	ENSP00000436676.1	E9PIB7
PRSS23	ENST00000532234.5	TSL:1	n.*1247G>A		3_prime_UTR	Exon 5 of 5	ENSP00000436676.1	E9PIB7

Frequencies

GnomAD3 genomes

AF:

0.0143

AC:

2176

AN:

152176

Hom.:

Cov.:

show subpopulations

Gnomad AFR

AF:

0.00941

Gnomad AMI

AF:

0.0175

Gnomad AMR

AF:

0.0223

Gnomad ASJ

AF:

0.0156

Gnomad EAS

AF:

0.000192

Gnomad SAS

AF:

0.0263

Gnomad FIN

AF:

0.00188

Gnomad MID

AF:

0.00633

Gnomad NFE

AF:

0.0177

Gnomad OTH

AF:

0.0120

GnomAD2 exomes

AF:

0.0184

AC:

4625

AN:

251466

AF XY:

0.0174

show subpopulations

Gnomad AFR exome

AF:

0.00880

Gnomad AMR exome

AF:

0.0474

Gnomad ASJ exome

AF:

0.0145

Gnomad EAS exome

AF:

0.000163

Gnomad FIN exome

AF:

0.00222

Gnomad NFE exome

AF:

0.0167

Gnomad OTH exome

AF:

0.0160

GnomAD4 exome

AF:

0.0188

AC:

27444

AN:

1461856

Hom.:

331

Cov.:

AF XY:

0.0184

AC XY:

13400

AN XY:

727228

show subpopulations

African (AFR)

AF:

0.00950

AC:

318

AN:

33480

American (AMR)

AF:

0.0434

AC:

1940

AN:

44724

Ashkenazi Jewish (ASJ)

AF:

0.0143

AC:

373

AN:

26136

East Asian (EAS)

AF:

0.000101

AC:

AN:

39700

South Asian (SAS)

AF:

0.0213

AC:

1841

AN:

86258

European-Finnish (FIN)

AF:

0.00264

AC:

141

AN:

53420

Middle Eastern (MID)

AF:

0.0104

AC:

AN:

5768

European-Non Finnish (NFE)

AF:

0.0196

AC:

21763

AN:

1111974

Other (OTH)

AF:

0.0166

AC:

1004

AN:

60396

Allele Balance Distribution

Red line indicates average allele balance

Average allele balance: 0.475

Heterozygous variant carriers

1475

2951

4426

5902

7377

0.00

0.20

0.40

0.60

0.80

0.95

Allele balance

Age Distribution

Exome Het

Exome Hom

Variant carriers

876

1752

2628

3504

4380

<30

30-35

35-40

40-45

45-50

50-55

55-60

60-65

65-70

70-75

75-80

>80

Age

GnomAD4 genome

AF:

0.0144

AC:

2186

AN:

152294

Hom.:

Cov.:

AF XY:

0.0140

AC XY:

1040

AN XY:

74486

show subpopulations

African (AFR)

AF:

0.00950

AC:

395

AN:

41572

American (AMR)

AF:

0.0222

AC:

340

AN:

15298

Ashkenazi Jewish (ASJ)

AF:

0.0156

AC:

AN:

3472

East Asian (EAS)

AF:

0.000193

AC:

AN:

5184

South Asian (SAS)

AF:

0.0261

AC:

126

AN:

4828

European-Finnish (FIN)

AF:

0.00188

AC:

AN:

10616

Middle Eastern (MID)

AF:

0.00680

AC:

AN:

294

European-Non Finnish (NFE)

AF:

0.0176

AC:

1200

AN:

68006

Other (OTH)

AF:

0.0152

AC:

AN:

2112

Allele Balance Distribution

Red line indicates average allele balance

Average allele balance: 0.502

Heterozygous variant carriers

114

227

341

454

568

0.00

0.20

0.40

0.60

0.80

0.95

Allele balance

Age Distribution

Genome Het

Genome Hom

Variant carriers

112

140

<30

30-35

35-40

40-45

45-50

50-55

55-60

60-65

65-70

70-75

75-80

>80

Age

Alfa

AF:

0.0178

Hom.:

Bravo

AF:

0.0162

TwinsUK

AF:

0.0235

AC:

ALSPAC

AF:

0.0158

AC:

ESP6500AA

AF:

0.00863

AC:

ESP6500EA

AF:

0.0180

AC:

155

ExAC

AF:

0.0166

AC:

2018

Asia WGS

AF:

0.0160

AC:

AN:

3478

EpiCase

AF:

0.0173

EpiControl

AF:

0.0169

ClinVar

ClinVar submissions

Significance:Benign

Revision:criteria provided, multiple submitters, no conflicts

View on ClinVar

Pathogenic

VUS

Benign

Condition

not provided (3)

Exudative vitreoretinopathy 1 (1)

not specified (1)

Computational scores

Source: dbNSFP v4.9

Name

Calibrated prediction

Score

Prediction

AlphaMissense

Benign

0.073

BayesDel_addAF

Benign

-0.32

BayesDel_noAF

Benign

-0.20

CADD

Uncertain

(source)

DANN

Uncertain

0.99

DEOGEN2

Benign

0.34

Eigen

Benign

0.026

Eigen_PC

Uncertain

0.27

FATHMM_MKL

Uncertain

0.94

LIST_S2

Benign

0.83

MetaRNN

Benign

0.0033

MetaSVM

Benign

-0.77

MutationAssessor

Benign

0.55

PhyloP100

6.5

PrimateAI

Uncertain

0.61

PROVEAN

Benign

-1.0

REVEL

Uncertain

0.33

Sift

Benign

0.086

Sift4G

Benign

0.071

Polyphen

0.15

Vest4

0.12

MPC

0.29

ClinPred

0.015

GERP RS

5.8

RBP_binding_hub_radar

0.0

RBP_regulation_power_radar

1.7

Varity_R

0.12

gMVP

0.56

Mutation Taster

=37/63

polymorphism (auto)

(source)

Splicing

Name

Calibrated prediction

Score

Prediction

SpliceAI score (max)

0.0

Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Publications

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Variant summary

Frequency

Consequence

Scores

Clinical Significance

Conservation

Publications

ACMG classification

Variant Effect in Transcripts

RefSeq Transcripts

Ensembl Transcripts

Frequencies

Age Distribution

Age Distribution

ClinVar

Computational scores

Splicing

Publications

Other links and lift over