rs140348243
Variant summary
Our verdict is Likely benign. Variant got -2 ACMG points: 2P and 4B. PP3_ModerateBS2
The NM_174934.4(SCN4B):c.617C>T(p.Ser206Leu) variant causes a missense change involving the alteration of a conserved nucleotide. The variant allele was found at a frequency of 0.000154 in 1,613,700 control chromosomes in the GnomAD database, with no homozygous occurrence. In-silico tool predicts a pathogenic outcome for this variant. Variant has been reported in ClinVar as Conflicting classifications of pathogenicity (no stars). Synonymous variant affecting the same amino acid position (i.e. S206S) has been classified as Likely benign.
Frequency
Consequence
NM_174934.4 missense
Scores
Clinical Significance
Conservation
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ACMG classification
Verdict is Likely_benign. Variant got -2 ACMG points.
Transcripts
RefSeq
Gene | Transcript | HGVSc | HGVSp | Effect | #exon/exons | MANE | UniProt |
---|---|---|---|---|---|---|---|
SCN4B | NM_174934.4 | c.617C>T | p.Ser206Leu | missense_variant | 5/5 | ENST00000324727.9 | |
SCN4B | NM_001142349.2 | c.287C>T | p.Ser96Leu | missense_variant | 4/4 | ||
SCN4B | NM_001142348.2 | c.215C>T | p.Ser72Leu | missense_variant | 3/3 | ||
SCN4B | NR_024527.2 | n.606C>T | non_coding_transcript_exon_variant | 4/4 |
Ensembl
Gene | Transcript | HGVSc | HGVSp | Effect | #exon/exons | TSL | MANE | Appris | UniProt |
---|---|---|---|---|---|---|---|---|---|
SCN4B | ENST00000324727.9 | c.617C>T | p.Ser206Leu | missense_variant | 5/5 | 1 | NM_174934.4 | P1 |
Frequencies
GnomAD3 genomes ? AF: 0.0000920 AC: 14AN: 152204Hom.: 0 Cov.: 32
GnomAD3 exomes AF: 0.0000756 AC: 19AN: 251394Hom.: 0 AF XY: 0.0000957 AC XY: 13AN XY: 135870
GnomAD4 exome AF: 0.000160 AC: 234AN: 1461496Hom.: 0 Cov.: 31 AF XY: 0.000166 AC XY: 121AN XY: 727074
GnomAD4 genome ? AF: 0.0000920 AC: 14AN: 152204Hom.: 0 Cov.: 32 AF XY: 0.0000941 AC XY: 7AN XY: 74358
ClinVar
Submissions by phenotype
Long QT syndrome 10 Uncertain:2
Uncertain significance, criteria provided, single submitter | clinical testing | Fulgent Genetics, Fulgent Genetics | Aug 31, 2021 | - - |
Uncertain significance, criteria provided, single submitter | clinical testing | Invitae | Nov 20, 2023 | This sequence change replaces serine, which is neutral and polar, with leucine, which is neutral and non-polar, at codon 206 of the SCN4B protein (p.Ser206Leu). This variant is present in population databases (rs140348243, gnomAD 0.01%). This missense change has been observed in individual(s) with sudden infant death and/or atrial fibrillation (PMID: 20226894, 30821358, 31043699). ClinVar contains an entry for this variant (Variation ID: 191380). An algorithm developed to predict the effect of missense changes on protein structure and function (PolyPhen-2) suggests that this variant is likely to be disruptive. Experimental studies have shown that this missense change affects SCN4B function (PMID: 20226894). In summary, the available evidence is currently insufficient to determine the role of this variant in disease. Therefore, it has been classified as a Variant of Uncertain Significance. - |
SUDDEN INFANT DEATH SYNDROME Uncertain:1
Uncertain significance, criteria provided, single submitter | research | Biesecker Lab/Clinical Genomics Section, National Institutes of Health | Apr 05, 2018 | - - |
not provided Uncertain:1
Uncertain significance, criteria provided, single submitter | clinical testing | GeneDx | Jan 06, 2022 | Reported in five-month-old African American male with sudden infant death syndrome (SIDS), and a 61-year-old Caucasian male reported to have a QTc interval of 452 msec (Tan et al., 2010; Ng et al., 2013); Observed in individuals with arrhythmia referred for genetic testing at GeneDx; to date, family studies have not been informative; In silico analysis supports that this missense variant has a deleterious effect on protein structure/function; Functional studies performed in two different model expression systems show that p.(S206L) alters cardiac sodium channel function by accentuating the late sodium current (Tan et al., 2010); Reported in ClinVar as a variant of uncertain significace (ClinVar Variant ID# 191380); This variant is associated with the following publications: (PMID: 23861362, 21215473, 21454796, 23304551, 23604097, 23465283, 20226894, 31043699) - |
Cardiovascular phenotype Benign:1
Likely benign, criteria provided, single submitter | clinical testing | Ambry Genetics | Feb 22, 2023 | This alteration is classified as likely benign based on a combination of the following: seen in unaffected individuals, population frequency, intact protein function, lack of segregation with disease, co-occurrence, RNA analysis, in silico models, amino acid conservation, lack of disease association in case-control studies, and/or the mechanism of disease or impacted region is inconsistent with a known cause of pathogenicity. - |
Computational scores
Source:
Splicing
Find out detailed SpliceAI scores and Pangolin per-transcript scores at